Mycobacterium phage Bread
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Detailed Information for Phage Bread
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBrea Murnan, Olivia DeFord
Year Found2017
Location FoundCrete, NE USA
Finding InstitutionDoane University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.62182 N, 96.949539 W Map
Discovery Notessoil patch was surrounded by small plants, very damp soil, many trees about 10 ft away, around 21°C, area shaded by trees, about 5cm below surface
Naming NotesContains Brea's first name and the first letter of Olivia's last name
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 16, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage600
Genome length (bp)153796
Character of genome endsCircularly Permuted
GC Content64.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionDoane University
Annotation StatusIn GenBank
Plaque NotesCapsid is 91.7nm in width, tail is 91.7nm in length
MorphotypeMyoviridae
Number of Genes230
Number of tRNAs31
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Bread_1

Bread_2

Bread_3
(nucleotidyltransferase)

Bread_4

Bread_5

Bread_6

Bread_7

Bread_8

Bread_9

Bread_10

Bread_11

Bread_12

Bread_13

Bread_14

Bread_15

Bread_16

Bread_17

Bread_18

Bread_19
(histidine triad nucleotide binding protein)

Bread_20

Bread_21

Bread_22

Bread_23

Bread_24

Bread_25

Bread_26

Bread_27

Bread_28

Bread_29

Bread_30
(band-7-like membrane protein)

Bread_31

Bread_32

Bread_33

Bread_34

Bread_35

Bread_36
(ribbon-helix-helix DNA binding protein)

Bread_37

Bread_38

Bread_39

Bread_40

Bread_41

Bread_42

Bread_43

Bread_44

Bread_45

Bread_46

Bread_47

Bread_48

Bread_49

Bread_50

Bread_51

Bread_52

Bread_53

Bread_54

Bread_55

Bread_56

Bread_57

Bread_58

Bread_59

Bread_60

Bread_61

Bread_62

Bread_63
(helix-turn-helix DNA binding domain protein)

Bread_64
(helix-turn-helix DNA binding protein)

Bread_65

Bread_66

Bread_67

Bread_68

Bread_69

Bread_70

Bread_71

Bread_72

Bread_73

Bread_74

Bread_75

Bread_76

Bread_77

Bread_78

Bread_79

Bread_80

Bread_81

Bread_82

Bread_83

Bread_84 — tRNA

Bread_85 — tRNA

Bread_86 — tRNA

Bread_87 — tRNA

Bread_88

Bread_89

Bread_90
(portal protein)

Bread_91

Bread_92

Bread_93
(ThyX-like thymidylate synthase)

Bread_94

Bread_95
(RNA ligase)

Bread_96

Bread_97

Bread_98

Bread_99
(major capsid protein)

Bread_100
(holliday junction resolvase)

Bread_101

Bread_102

Bread_103

Bread_104

Bread_105

Bread_106

Bread_107

Bread_108

Bread_109

Bread_110

Bread_111

Bread_112

Bread_113

Bread_114

Bread_115

Bread_116

Bread_117

Bread_118

Bread_119

Bread_120

Bread_121

Bread_122

Bread_123

Bread_124

Bread_125

Bread_126
(tail sheath protein)

Bread_127

Bread_128

Bread_129
(tail assembly chaperone)

Bread_130
(tail assembly chaperone)

Bread_131
(tape measure protein)

Bread_132

Bread_133

Bread_134

Bread_135
(minor tail protein)

Bread_136

Bread_137
(minor tail protein)

Bread_138
(baseplate wedge protein)

Bread_139
(baseplate J protein)

Bread_140

Bread_141

Bread_142

Bread_143

Bread_144
(minor tail protein)

Bread_145

Bread_146

Bread_147

Bread_148

Bread_149

Bread_150

Bread_151

Bread_152 — tRNA

Bread_153 — tRNA

Bread_154

Bread_155
(peptidyl tRNA hydrolase)

Bread_156 — tRNA

Bread_157 — tRNA

Bread_158 — tRNA

Bread_159 — tRNA

Bread_160 — tRNA

Bread_161 — tRNA

Bread_162 — tRNA

Bread_163 — tRNA

Bread_164 — tRNA

Bread_165 — tRNA

Bread_166 — tRNA

Bread_167 — tRNA

Bread_168 — tRNA

Bread_169 — tRNA

Bread_170
(minor tail protein)

Bread_171

Bread_172 — tRNA

Bread_173 — tRNA

Bread_174 — tRNA

Bread_175 — tRNA

Bread_176 — tRNA

Bread_177 — tRNA

Bread_178 — tRNA

Bread_179 — tmRNA

Bread_180 — tRNA

Bread_181 — tRNA

Bread_182

Bread_183
(DNA helicase)

Bread_184

Bread_185

Bread_186

Bread_187

Bread_188

Bread_189

Bread_190

Bread_191

Bread_192

Bread_193
(thymidylate kinase)

Bread_194
(aminotransferase)

Bread_195

Bread_196

Bread_197
(DnaC-like helicase loader)

Bread_198
(DnaB-like dsDNA helicase)

Bread_199

Bread_200
(DNA primase)

Bread_201

Bread_202
(DnaJ-like chaperonin)

Bread_203
(ssDNA binding protein)

Bread_204
(DnaE-like DNA polymerase III)

Bread_205
(RF-1 peptide chain release factor)

Bread_206
(RecA-like DNA recombinase)

Bread_207
(holliday junction resolvase)

Bread_208

Bread_209
(HNH endonuclease)

Bread_210
(RusA-like resolvase)

Bread_211

Bread_212

Bread_213

Bread_214

Bread_215

Bread_216

Bread_217

Bread_218 — tRNA

Bread_219 — tRNA

Bread_220

Bread_221

Bread_222

Bread_223

Bread_224

Bread_225

Bread_226

Bread_227

Bread_228
(Ro-like RNA binding protein)

Bread_229

Bread_230

Bread_231

Bread_232

Bread_233

Bread_234

Bread_235

Bread_236
(glycosyltransferase)

Bread_237

Bread_238
(glycosyltransferase)

Bread_239
(glycosyltransferase)

Bread_240
(PnuC-like nicotinamide riboside transporter)

Bread_241
(glycosyltransferase)

Bread_242

Bread_243

Bread_244

Bread_245

Bread_246
(HNH endonuclease)

Bread_247
(lysin A)

Bread_248
(holin)

Bread_249
(lysin B)

Bread_250
(terminase)

Bread_251

Bread_252

Bread_253

Bread_254

Bread_255
(polynucleotide kinase)

Bread_256

Bread_257
(serine/threonine kinase)

Bread_258

Bread_259
(PurA-like adenylosuccinate synthetase)

Bread_260

Bread_261

Bread_262

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH779498
Refseq NumberNone yet
Published in PaperDoyle et al 2019, Microbiology Resource Announcements: Genome Sequences of Three Cluster C Mycobacteriophages, Bipolarisk, Bread, and FudgeTart
Archiving Info
Archiving status Archived
Pitt Freezer Box# 66
Pitt Freezer Box Grid# B8
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload