| Detailed Information for Phage Colt |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Haley Mulder and James Gonzalez |
| Year Found | 2015 |
| Location Found | Corpus Christi, TX USA |
| Finding Institution | Del Mar College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 27.69 N, 97.3784 W Map |
| Discovery Notes | Soil sample was taken in a horse pasture near a small pond on August 31, 2015 at 9:05 AM. Sample was taken 1 1/2 inches under the surface. Sample is a gray clay with small amount of gravel in the soil. Soil contains some algae and has a moist composition. Weather condition was overcast with a slight wind at 1mph, 85% humidity, 0% precipitation, and it was 80 degrees F outside. |
| Naming Notes | Phage was named after my miniature horse named Colt. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 28, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 907 |
| Genome length (bp) | 158508 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Final DNA concentration identified through spectrophotometry: 217.6 Ng/nl |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Subsequent to serial dilution, spot assays and streak tests, the putative plaque which was isolated and further refined through MTL & HTL protocols produced lytic plaques which were approximately 0.5 mm in diameter, consistent in morphology throughout the area of the agar plate and also consistent in opacity. The area of the individual plaques did not exceed the aforementioned measurement, and were robust in quantity. |
| Morphotype | Myoviridae |
| Number of Genes | 223 |
| Number of tRNAs | 33 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK359359 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Date of SEA Lysate Titering |
Nov 9, 2015 |
| Pitt Freezer Box# |
23 |
| Pitt Freezer Box Grid# |
H11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
