Mycobacterium phage DirtMonster
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Detailed Information for Phage DirtMonster
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesAJQB 1.6.2A
Found ByAlexander Blackwell
Year Found2021
Location FoundAuckland, New Zealand
Finding InstitutionMassey University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates36.768056 S, 174.746944 E Map
Discovery NotesDirtMonster was collected from a compost bin, the sample was taken under grass clippings and leaf litter on the top layer of the compost bin. When the sample was collected it was warm and damp, the sample was collected at 5:45pm on the 24th of February 2021.
Naming NotesDirtMonster was named after the nickname for bacteriophages my partner made up after my attempt to explain what I am doing in the course during the semester.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 19, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage509
Genome length (bp)154862
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionMassey University
Annotation StatusIn GenBank
Plaque NotesPlaques are clear, round and are approximately 1mm in diameter.
MorphotypeMyoviridae
Number of Genes221
Number of tRNAs34
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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DirtMonster_1

DirtMonster_2

DirtMonster_3
(LAGLIDADG endonuclease)

DirtMonster_4

DirtMonster_5

DirtMonster_6

DirtMonster_7

DirtMonster_8

DirtMonster_9

DirtMonster_10

DirtMonster_11

DirtMonster_12

DirtMonster_13

DirtMonster_14

DirtMonster_15

DirtMonster_16
(histidine triad nucleotide binding protein)

DirtMonster_17

DirtMonster_18

DirtMonster_19

DirtMonster_20

DirtMonster_21

DirtMonster_22

DirtMonster_23

DirtMonster_24
(HNH endonuclease)

DirtMonster_25

DirtMonster_26

DirtMonster_27

DirtMonster_28

DirtMonster_29

DirtMonster_30

DirtMonster_31

DirtMonster_32

DirtMonster_33

DirtMonster_34

DirtMonster_35

DirtMonster_36

DirtMonster_37

DirtMonster_38

DirtMonster_39

DirtMonster_40

DirtMonster_41

DirtMonster_42

DirtMonster_43

DirtMonster_44

DirtMonster_45

DirtMonster_46

DirtMonster_47

DirtMonster_48

DirtMonster_49

DirtMonster_50

DirtMonster_51

DirtMonster_52

DirtMonster_53

DirtMonster_54

DirtMonster_55

DirtMonster_56

DirtMonster_57

DirtMonster_58

DirtMonster_59
(helix-turn-helix DNA binding protein)

DirtMonster_60
(helix-turn-helix DNA binding protein)

DirtMonster_61

DirtMonster_62

DirtMonster_63

DirtMonster_64

DirtMonster_65

DirtMonster_66

DirtMonster_67

DirtMonster_68

DirtMonster_69

DirtMonster_70

DirtMonster_71

DirtMonster_72

DirtMonster_73

DirtMonster_74

DirtMonster_75

DirtMonster_76

DirtMonster_77 — tRNA
(tRNA-Ser(gct))

DirtMonster_78 — tRNA
(tRNA-Leu(cag))

DirtMonster_79 — tRNA
(tRNA-Leu(gag))

DirtMonster_80 — tRNA
(tRNA-Leu(caa))

DirtMonster_81

DirtMonster_82

DirtMonster_83
(portal protein)

DirtMonster_84

DirtMonster_85

DirtMonster_86
(ThyX-like thymidylate synthase)

DirtMonster_87

DirtMonster_88
(RNA ligase)

DirtMonster_89
(HNH endonuclease)

DirtMonster_90

DirtMonster_91

DirtMonster_92

DirtMonster_93
(major capsid protein)

DirtMonster_94

DirtMonster_95

DirtMonster_96

DirtMonster_97

DirtMonster_98

DirtMonster_99

DirtMonster_100

DirtMonster_101

DirtMonster_102

DirtMonster_103

DirtMonster_104

DirtMonster_105

DirtMonster_106

DirtMonster_107

DirtMonster_108

DirtMonster_109

DirtMonster_110

DirtMonster_111

DirtMonster_112

DirtMonster_113

DirtMonster_114

DirtMonster_115
(acetyltransferase)

DirtMonster_116

DirtMonster_117

DirtMonster_118
(ParB-like nuclease domain protein)

DirtMonster_119

DirtMonster_120

DirtMonster_121
(tail sheath protein)

DirtMonster_122

DirtMonster_123

DirtMonster_124
(tail assembly chaperone)

DirtMonster_125
(tail assembly chaperone)

DirtMonster_126
(tape measure protein)

DirtMonster_127
(minor tail protein)

DirtMonster_128

DirtMonster_129

DirtMonster_130
(minor tail protein)

DirtMonster_131
(minor tail protein)

DirtMonster_132
(minor tail protein)

DirtMonster_133
(baseplate wedge protein)

DirtMonster_134
(baseplate wedge protein)

DirtMonster_135

DirtMonster_136

DirtMonster_137

DirtMonster_138
(minor tail protein)

DirtMonster_139
(minor tail protein)

DirtMonster_140

DirtMonster_141

DirtMonster_142

DirtMonster_143

DirtMonster_144

DirtMonster_145

DirtMonster_146

DirtMonster_147

DirtMonster_148 — tRNA
(tRNA-Pro(tgg))

DirtMonster_149 — tRNA
(tRNA-Trp(cca))

DirtMonster_150 — tRNA
(tRNA-Tyr(gta))

DirtMonster_151
(peptidyl tRNA hydrolase)

DirtMonster_152 — tRNA
(tRNA-Pyl(cta))

DirtMonster_153 — tRNA
(tRNA-Met(cat))

DirtMonster_154 — tRNA
(tRNA-Cys(gca))

DirtMonster_155 — tRNA
(tRNA-Glu(ctc))

DirtMonster_156 — tRNA
(tRNA-His(gtg))

DirtMonster_157 — tRNA
(tRNA-Ala(tgc))

DirtMonster_158 — tRNA
(tRNA-Phe(gaa))

DirtMonster_159 — tRNA
(tRNA-Val(cac))

DirtMonster_160 — tRNA
(tRNA-Lys(ctt))

DirtMonster_161 — tRNA
(tRNA-Glu(ttc))

DirtMonster_162 — tRNA
(tRNA-Gly(tcc))

DirtMonster_163 — tRNA
(tRNA-Thr(cgt))

DirtMonster_164 — tRNA
(tRNA-Thr(tgt))

DirtMonster_165 — tRNA
(tRNA-Thr(ggt))

DirtMonster_166
(minor tail protein)

DirtMonster_167

DirtMonster_168 — tRNA
(tRNA-Gly(gcc))

DirtMonster_169 — tRNA
(tRNA-Asp(gtc))

DirtMonster_170 — tRNA
(tRNA-Met(cat))

DirtMonster_171 — tRNA
(tRNA-Ile(gat))

DirtMonster_172 — tRNA
(tRNA-Arg(acg))

DirtMonster_173 — tRNA
(tRNA-Val(gac))

DirtMonster_174 — tRNA
(tRNA-Arg(ccg))

DirtMonster_175 — tRNA
(tRNA-Arg(cct))

DirtMonster_176 — tmRNA
(Tag peptide: ATDTDATVTDAEIEAFFAEEAAALV*)

DirtMonster_177 — tRNA
(tRNA-Gln(ttg))

DirtMonster_178 — tRNA
(tRNA-Arg(tct))

DirtMonster_179 — tRNA
(tRNA-Lys(ttt))

DirtMonster_180

DirtMonster_181
(DNA helicase)

DirtMonster_182

DirtMonster_183

DirtMonster_184

DirtMonster_185

DirtMonster_186

DirtMonster_187

DirtMonster_188

DirtMonster_189

DirtMonster_190

DirtMonster_191
(thymidylate kinase)

DirtMonster_192
(aminotransferase)

DirtMonster_193

DirtMonster_194

DirtMonster_195
(DnaC-like helicase loader)

DirtMonster_196
(DnaB-like dsDNA helicase)

DirtMonster_197

DirtMonster_198
(DNA primase)

DirtMonster_199

DirtMonster_200
(DnaJ-like chaperonin)

DirtMonster_201
(SSB protein)

DirtMonster_202
(DnaE-like DNA polymerase III alpha)

DirtMonster_203
(RF-1 peptide chain release factor)

DirtMonster_204
(RecA-like DNA recombinase)

DirtMonster_205
(Holliday junction resolvase)

DirtMonster_206

DirtMonster_207
(HNH endonuclease)

DirtMonster_208
(RusA-like resolvase)

DirtMonster_209

DirtMonster_210

DirtMonster_211

DirtMonster_212

DirtMonster_213

DirtMonster_214

DirtMonster_215

DirtMonster_216 — tRNA
(tRNA-Gln(ctg))

DirtMonster_217 — tRNA
(tRNA-Asn(gtt))

DirtMonster_218

DirtMonster_219

DirtMonster_220

DirtMonster_221

DirtMonster_222

DirtMonster_223

DirtMonster_224
(Ro-like RNA binding protein)

DirtMonster_225

DirtMonster_226

DirtMonster_227

DirtMonster_228

DirtMonster_229

DirtMonster_230
(glycosyltransferase)

DirtMonster_231
(glycosyltransferase)

DirtMonster_232
(glycosyltransferase)

DirtMonster_233
(PnuC-like nicotinamide riboside transporter)

DirtMonster_234
(glycosyltransferase)

DirtMonster_235

DirtMonster_236

DirtMonster_237

DirtMonster_238

DirtMonster_239
(lysin A N-acetylmuramoyl-L-alanine amidase domain)

DirtMonster_240
(holin)

DirtMonster_241
(lysin B)

DirtMonster_242
(terminase large subunit)

DirtMonster_243

DirtMonster_244

DirtMonster_245

DirtMonster_246

DirtMonster_247
(polynucleotide kinase)

DirtMonster_248

DirtMonster_249

DirtMonster_250
(serine/threonine kinase)

DirtMonster_251

DirtMonster_252

DirtMonster_253
(PurA-like adenylosuccinate synthetase)

DirtMonster_254

DirtMonster_255

DirtMonster_256

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP405131
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 130
Pitt Freezer Box Grid# G11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload