Mycobacterium phage EasyJones
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Detailed Information for Phage EasyJones
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEmir Sean Ishwar Jones
Year Found2019
Location FoundBaltimore, MD USA
Finding InstitutionHoward Hughes Medical Institute
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates39.2553 N, 76.7144 W Map
Discovery NotesSoil sample collected on UMBC campus, at a depth of ~ 2 cm on a garden bed next to plants. The soil sample was moderately dry.
Naming NotesThe phage is names after the discoverer's initials, which are ESI and pronounced "easy".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 16, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage221
Genome length (bp)154315
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionHoward Hughes Medical Institute
Annotation StatusIn GenBank
Plaque NotesAfter 24hrs of incubation at 37C, small clear plaques of ~ 0.5mm in diameter are formed. When left at room temperature for an additional 4 days, plaques are ~ 2mm in diameter, remain clear, but develop a halo that appears more dense than the surrounding bacteria. See attached image.
MorphotypeMyoviridae
Number of Genes232
Number of tRNAs34
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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EasyJones_1

EasyJones_2

EasyJones_3
(nucleotidyltransferase)

EasyJones_4

EasyJones_5

EasyJones_6

EasyJones_7

EasyJones_8

EasyJones_9

EasyJones_10

EasyJones_11

EasyJones_12

EasyJones_13

EasyJones_14

EasyJones_15

EasyJones_16

EasyJones_17

EasyJones_18
(histidine triad nucleotide binding protein)

EasyJones_19

EasyJones_20

EasyJones_21

EasyJones_22

EasyJones_23

EasyJones_24

EasyJones_25

EasyJones_26

EasyJones_27

EasyJones_28
(band-7-like membrane protein)

EasyJones_29

EasyJones_30

EasyJones_31

EasyJones_32

EasyJones_33

EasyJones_34

EasyJones_35

EasyJones_36

EasyJones_37

EasyJones_38

EasyJones_39

EasyJones_40

EasyJones_41

EasyJones_42

EasyJones_43

EasyJones_44

EasyJones_45

EasyJones_46

EasyJones_47

EasyJones_48

EasyJones_49
(immunity repressor)

EasyJones_50

EasyJones_51

EasyJones_52

EasyJones_53

EasyJones_54

EasyJones_55

EasyJones_56

EasyJones_57

EasyJones_58

EasyJones_59

EasyJones_60

EasyJones_61

EasyJones_62

EasyJones_63

EasyJones_64

EasyJones_65
(helix-turn-helix DNA binding domain protein)

EasyJones_66
(helix-turn-helix DNA binding protein)

EasyJones_67

EasyJones_68

EasyJones_69

EasyJones_70

EasyJones_71

EasyJones_72

EasyJones_73

EasyJones_74

EasyJones_75

EasyJones_76

EasyJones_77

EasyJones_78

EasyJones_79

EasyJones_80

EasyJones_81

EasyJones_82

EasyJones_83

EasyJones_84

EasyJones_85

EasyJones_86 — tRNA
(tRNA-Ser(gct))

EasyJones_87 — tRNA
(tRNA-Ser(cga))

EasyJones_88 — tRNA
(tRNA-Leu(cag))

EasyJones_89 — tRNA
(tRNA-Leu(gag))

EasyJones_90 — tRNA
(tRNA-Leu(caa))

EasyJones_91

EasyJones_92

EasyJones_93
(portal protein)

EasyJones_94

EasyJones_95

EasyJones_96
(ThyX-like thymidylate synthase)

EasyJones_97

EasyJones_98
(RNA ligase)

EasyJones_99

EasyJones_100

EasyJones_101

EasyJones_102
(major capsid protein)

EasyJones_103
(holliday junction resolvase)

EasyJones_104

EasyJones_105
(head fiber protein)

EasyJones_106

EasyJones_107

EasyJones_108

EasyJones_109

EasyJones_110

EasyJones_111

EasyJones_112

EasyJones_113

EasyJones_114

EasyJones_115

EasyJones_116

EasyJones_117

EasyJones_118

EasyJones_119

EasyJones_120

EasyJones_121

EasyJones_122

EasyJones_123

EasyJones_124

EasyJones_125

EasyJones_126

EasyJones_127

EasyJones_128

EasyJones_129

EasyJones_130
(tail sheath protein)

EasyJones_131

EasyJones_132

EasyJones_133
(tail assembly chaperone)

EasyJones_134
(tail assembly chaperone)

EasyJones_135
(tape measure protein)

EasyJones_136

EasyJones_137

EasyJones_138

EasyJones_139
(minor tail protein)

EasyJones_140
(minor tail protein)

EasyJones_141
(minor tail protein)

EasyJones_142
(baseplate wedge protein)

EasyJones_143
(baseplate wedge protein)

EasyJones_144

EasyJones_145

EasyJones_146

EasyJones_147
(minor tail protein)

EasyJones_148
(minor tail protein)

EasyJones_149

EasyJones_150

EasyJones_151

EasyJones_152

EasyJones_153

EasyJones_154

EasyJones_155

EasyJones_156

EasyJones_157 — tRNA
(tRNA-Pro(tgg))

EasyJones_158 — tRNA
(tRNA-Trp(cca))

EasyJones_159 — tRNA
(tRNA-Tyr(gta))

EasyJones_160

EasyJones_161
(peptidyl tRNA hydrolase)

EasyJones_162 — tRNA
(tRNA-Pyl(cta))

EasyJones_163 — tRNA
(tRNA-Met(cat))

EasyJones_164 — tRNA
(tRNA-Cys(gca))

EasyJones_165 — tRNA
(tRNA-Glu(ctc))

EasyJones_166 — tRNA
(tRNA-His(gtg))

EasyJones_167 — tRNA
(tRNA-Ala(tgc))

EasyJones_168 — tRNA
(tRNA-Phe(gaa))

EasyJones_169 — tRNA
(tRNA-Val(cac))

EasyJones_170 — tRNA
(tRNA-Lys(ctt))

EasyJones_171 — tRNA
(tRNA-Glu(ttc))

EasyJones_172 — tRNA
(tRNA-Gly(tcc))

EasyJones_173 — tRNA
(tRNA-Thr(cgt))

EasyJones_174 — tRNA
(tRNA-Thr(tgt))

EasyJones_175
(HNH endonuclease)

EasyJones_176 — tRNA
(tRNA-Thr(ggt))

EasyJones_177
(minor tail protein)

EasyJones_178

EasyJones_179 — tRNA
(tRNA-Gly(gcc))

EasyJones_180 — tRNA
(tRNA-Asp(gtc))

EasyJones_181 — tRNA
(tRNA-Met(cat))

EasyJones_182 — tRNA
(tRNA-Ile(gat))

EasyJones_183 — tRNA
(tRNA-Arg(acg))

EasyJones_184 — tRNA
(tRNA-Val(gac))

EasyJones_185 — tRNA
(tRNA-Arg(cct))

EasyJones_186 — tmRNA
(Tag peptide: ATDTDATVTDAEIEAFFAEEAAALV*)

EasyJones_187 — tRNA
(tRNA-Gln(ttg))

EasyJones_188 — tRNA
(tRNA-Arg(tct))

EasyJones_189 — tRNA
(tRNA-Lys(ttt))

EasyJones_190

EasyJones_191

EasyJones_192
(DNA helicase)

EasyJones_193

EasyJones_194

EasyJones_195

EasyJones_196

EasyJones_197

EasyJones_198

EasyJones_199

EasyJones_200

EasyJones_201
(thymidylate kinase)

EasyJones_202
(aminotransferase)

EasyJones_203

EasyJones_204

EasyJones_205
(DnaC-like helicase loader)

EasyJones_206
(DnaB-like dsDNA helicase)

EasyJones_207

EasyJones_208
(DNA primase)

EasyJones_209

EasyJones_210
(DnaJ-like chaperonin)

EasyJones_211
(ssDNA binding protein)

EasyJones_212

EasyJones_213
(RF-1 peptide chain release factor)

EasyJones_214
(RecA-like DNA recombinase)

EasyJones_215
(holliday junction resolvase)

EasyJones_216

EasyJones_217
(Cas4 family exonuclease)

EasyJones_218
(RusA-like resolvase)

EasyJones_219

EasyJones_220

EasyJones_221

EasyJones_222

EasyJones_223
(hydrolase)

EasyJones_224

EasyJones_225

EasyJones_226 — tRNA
(tRNA-Gln(ctg))

EasyJones_227 — tRNA
(tRNA-Asn(gtt))

EasyJones_228

EasyJones_229

EasyJones_230

EasyJones_231

EasyJones_232

EasyJones_233

EasyJones_234
(Ro-like RNA binding protein)

EasyJones_235

EasyJones_236

EasyJones_237

EasyJones_238

EasyJones_239

EasyJones_240

EasyJones_241
(glycosyltransferase)

EasyJones_242

EasyJones_243
(glycosyltransferase)

EasyJones_244
(glycosyltransferase)

EasyJones_245
(PnuC-like nicotinamide riboside transporter)

EasyJones_246
(glycosyltransferase)

EasyJones_247

EasyJones_248

EasyJones_249

EasyJones_250

EasyJones_251
(lysin A)

EasyJones_252
(holin)

EasyJones_253
(lysin B)

EasyJones_254
(terminase)

EasyJones_255

EasyJones_256

EasyJones_257

EasyJones_258

EasyJones_259
(polynucleotide kinase)

EasyJones_260

EasyJones_261
(serine/threonine kinase)

EasyJones_262

EasyJones_263

EasyJones_264
(PurA-like adenylosuccinate synthetase)

EasyJones_265

EasyJones_266

EasyJones_267

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ856343
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 96
Pitt Freezer Box Grid# G12
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload