Mycobacterium phage Ghost
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Detailed Information for Phage Ghost
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesDjDiddles
Found ByJessica Norris
Year Found2009
Location FoundRadnor, PA USA
Finding InstitutionCabrini University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.0547 N, 75.3719 W Map
Discovery NotesMulchy, and underneath a tree
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 4, 2010
Genome length (bp)155167
Character of genome endsCircularly Permuted
GC Content64.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPinhole plaques that are clear. 1mm approximately. Took 24 hours to grow at 37 degrees Celsius
MorphotypeMyoviridae
Number of Genes231
Number of tRNAs34
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Ghost_1

Ghost_2

Ghost_3
(nucleotidyl transferase)

Ghost_4

Ghost_5

Ghost_6

Ghost_7

Ghost_8

Ghost_9

Ghost_10

Ghost_11

Ghost_12

Ghost_13

Ghost_14

Ghost_15

Ghost_16

Ghost_17

Ghost_18

Ghost_19

Ghost_20

Ghost_21

Ghost_22

Ghost_23

Ghost_24

Ghost_25

Ghost_26

Ghost_27

Ghost_28

Ghost_29
(band-7-like membrane protein)

Ghost_30

Ghost_31

Ghost_32

Ghost_33

Ghost_34

Ghost_35

Ghost_36

Ghost_37

Ghost_38

Ghost_39

Ghost_40

Ghost_41

Ghost_42

Ghost_43

Ghost_44

Ghost_45

Ghost_46

Ghost_47

Ghost_48

Ghost_49

Ghost_50

Ghost_51

Ghost_52

Ghost_53

Ghost_54

Ghost_55

Ghost_56

Ghost_57

Ghost_58

Ghost_59

Ghost_60

Ghost_61

Ghost_62
(helix-turn-helix DNA binding domain protein)

Ghost_63

Ghost_64

Ghost_65

Ghost_66

Ghost_67

Ghost_68

Ghost_69

Ghost_70

Ghost_71

Ghost_72

Ghost_73

Ghost_74

Ghost_75

Ghost_76

Ghost_77

Ghost_78

Ghost_79

Ghost_80

Ghost_81

Ghost_82

Ghost_83 — tRNA
(tRNA-Ser (gct))

Ghost_84 — tRNA
(tRNA-Leu (cag))

Ghost_85 — tRNA
(tRNA-Leu (gag))

Ghost_86 — tRNA
(tRNA-Leu (caa))

Ghost_87

Ghost_88

Ghost_89

Ghost_90

Ghost_91

Ghost_92
(ThyX-like thymidylate synthase)

Ghost_93

Ghost_94
(RNA ligase)

Ghost_95

Ghost_96

Ghost_97

Ghost_98

Ghost_99

Ghost_100

Ghost_101

Ghost_102

Ghost_103

Ghost_104

Ghost_105

Ghost_106

Ghost_107

Ghost_108

Ghost_109

Ghost_110

Ghost_111

Ghost_112

Ghost_113

Ghost_114

Ghost_115

Ghost_116

Ghost_117

Ghost_118

Ghost_119

Ghost_120

Ghost_121

Ghost_122

Ghost_123

Ghost_124

Ghost_125

Ghost_126
(tail sheath protein)

Ghost_127

Ghost_128

Ghost_129
(tail assembly chaperone)

Ghost_130
(tail assembly chaperone)

Ghost_131
(tape measure protein)

Ghost_132

Ghost_133

Ghost_134

Ghost_135
(minor tail protein)

Ghost_136

Ghost_137
(minor tail protein)

Ghost_138

Ghost_139
(baseplate J protein)

Ghost_140

Ghost_141

Ghost_142

Ghost_143

Ghost_144
(minor tail protein)

Ghost_145

Ghost_146

Ghost_147

Ghost_148

Ghost_149

Ghost_150

Ghost_151

Ghost_152

Ghost_153 — tRNA
(tRNA-Pro (tgg))

Ghost_154 — tRNA
(tRNA-Trp (cca))

Ghost_155 — tRNA
(tRNA-Tyr (gta))

Ghost_156

Ghost_157
(peptidyl tRNA hydrolase)

Ghost_158 — tRNA
(tRNA-suppressor (gta))

Ghost_159 — tRNA
(tRNA-Met (cat))

Ghost_160 — tRNA
(tRNA-Cys (gca))

Ghost_161 — tRNA
(tRNA-Glu (ctc))

Ghost_162 — tRNA
(tRNA-His (gtg))

Ghost_163 — tRNA
(tRNA-Ala (tgc))

Ghost_164 — tRNA
(tRNA-Phe (gaa))

Ghost_165 — tRNA
(tRNA-Val (cac))

Ghost_166 — tRNA
(tRNA-Lys (ctt))

Ghost_167 — tRNA
(tRNA-Glu (ttc))

Ghost_168 — tRNA
(tRNA-Gly (tcc))

Ghost_169 — tRNA
(tRNA-Thr (cgt))

Ghost_170 — tRNA
(tRNA-Thr (tgt))

Ghost_171
(HNH endonuclease)

Ghost_172 — tRNA
(tRNA-Thr (gtt))

Ghost_173
(minor tail protein)

Ghost_174

Ghost_175 — tRNA
(tRNA-Gly (gcc))

Ghost_176 — tRNA
(tRNA-Asp (gtc))

Ghost_177 — tRNA
(tRNA-Met (cat))

Ghost_178 — tRNA
(tRNA-Ile (gat))

Ghost_179 — tRNA
(tRNA-Arg (acg))

Ghost_180 — tRNA
(tRNA-Val (gac))

Ghost_181 — tRNA
(gp181; tRNA-Gly (tcc))

Ghost_182 — tRNA
(tRNA-Arg (cct))

Ghost_183 — tmRNA

Ghost_184 — tRNA
(tRNA-Gln (ttg))

Ghost_185 — tRNA
(tRNA-Arg (tct))

Ghost_186 — tRNA
(gp186; tRNA-Lys (ttt))

Ghost_187

Ghost_188
(DNA helicase)

Ghost_189

Ghost_190

Ghost_191

Ghost_192

Ghost_193

Ghost_194

Ghost_195

Ghost_196

Ghost_197

Ghost_198
(aminotransferase)

Ghost_199

Ghost_200

Ghost_201

Ghost_202
(DnaC-like helicase loader)

Ghost_203
(DnaB-like dsDNA helicase)

Ghost_204

Ghost_205
(DNA primase)

Ghost_206

Ghost_207
(DnaJ-like chaperonin)

Ghost_208

Ghost_209
(DnaE-like DNA polymerase III)

Ghost_210
(RF-1 peptide chain release factor)

Ghost_211
(RecA-like DNA recombinase)

Ghost_212

Ghost_213

Ghost_214

Ghost_215
(RusA-like resolvase)

Ghost_216

Ghost_217

Ghost_218

Ghost_219

Ghost_220

Ghost_221

Ghost_222

Ghost_223

Ghost_224 — tRNA
(tRNA-Gln (ctg))

Ghost_225 — tRNA
(tRNA-Asn (gtt))

Ghost_226

Ghost_227

Ghost_228

Ghost_229

Ghost_230

Ghost_231

Ghost_232
(Ro-like RNA binding protein)

Ghost_233

Ghost_234

Ghost_235

Ghost_236

Ghost_237

Ghost_238

Ghost_239

Ghost_240
(glycosyltransferase)

Ghost_241

Ghost_242

Ghost_243
(glycosyltransferase)

Ghost_244
(PnuC-like nicotinamide riboside transporter)

Ghost_245
(glycosyltransferase)

Ghost_246

Ghost_247

Ghost_248

Ghost_249

Ghost_250
(lysin A)

Ghost_251
(holin)

Ghost_252
(lysin B)

Ghost_253
(terminase)

Ghost_254

Ghost_255

Ghost_256

Ghost_257

Ghost_258
(polynucleotide kinase)

Ghost_259

Ghost_260
(serine/threonine kinase)

Ghost_261

Ghost_262

Ghost_263
(PurA-like adenylosuccinate synthetase)

Ghost_264

Ghost_265

Ghost_266

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionJF704096
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2009CABRGhostJN
SEA Lysate Titer 1e10
Pitt Freezer Box# 1
Pitt Freezer Box Grid# 20
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Virtual Digest PictureDownload
EM PictureDownload