| Detailed Information for Phage Greely |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Michelle Levesque |
| Year Found | 2023 |
| Location Found | Greely, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 23°C |
| GPS Coordinates | 45.261003 N, 75.568576 W Map |
| Discovery Notes | This phage was isolated from a moist and dark soil sample. The soil was collected roughly 3-5cm beneath the surface from a garden in Greely, ON. This garden had recently been topped off with cow manure. The soil sample was collected on September 24, 2023. |
| Naming Notes | This phage was named after the rural community where the soil sample was collected. Greely is located in Ottawa, ON. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 241 |
| Genome length (bp) | 164941 |
| Character of genome ends | Circularly Permuted |
| GC Content | 65.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Ottawa |
| Annotation Status | Overdue (Expected completion by 5/1/2025) |
| Plaque Notes | Very tiny clear plaques. |
| Morphotype | Myoviridae |
| Number of Genes | 247 |
| Number of tRNAs | 33 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
176 |
| Pitt Freezer Box Grid# |
C11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
