The Actinobacteriophage Database

Mycobacterium phage Grungle

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Detailed Information for Phage Grungle
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Melissa Folkerts
Year Found	2018
Location Found	Los Angeles, CA USA
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	No
Isolation Temperature	30°C
GPS Coordinates	34.066687 N, 118.451216 W Map
Discovery Notes	Phage Grungle was isolated from Damp, dark, rich soil collected on April 11, 2018 by Melissa Folkerts at a depth of about 4 inches. Air temp at time of collection was 16.7˚C. Grungle was isolated by direct plating at 30˚C. Characterization was performed by Team Plaque Donald's Big Plaques (Richmund Tan, Melissa Folkerts, Lovelyn Edillo, Neiki Rokni), Team B.KFC (Kimberly Uehisa, Brooke Crosswhite, Christopher Huerta, Fangkun Li), and Team KARL (Karina Haleblian, Alexis Ballew, Lillian Lai, Rinah Kim). 103BL-S18 Instructor: Dr. Amanda Freise Teaching Assistant: Samuel Wu Undergraduate Assistants: Mariam Khachatrian, Kalin Patel Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker
Naming Notes	One of PDBP's group members read a story about a person having a dream that they were at Hogwarts and the sorting hat grouped them into "Grunglebunk." This inspired the name Grungle.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Aug 3, 2018
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Approximate Shotgun Coverage	527
Genome length (bp)	153739
Character of genome ends	Circularly Permuted
GC Content	64.6%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	C
Subcluster	C1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Ading (C1) Adlitam (C1) Alice (C1) Amataga (C1) ArcherS7 (C1) Asterius (C1) Astraea (C1) Atlantean (C1) Audrick (C1) Ava3 (C1) Babyland (C1) BackyardAgain (C1) BadAgartude (C1) BananaFence (C1) Bangla1971 (C1) Basquiat (C1) BeanWater (C1) BigCity (C1) Bigswole (C1) Bipolarisk (C1) Blackbrain (C1) Blackdragon (C1) Blubba (C1) Bonray (C1) Bread (C1) Breeniome (C1) Burrough (C1) Bxz1 (C1) Cactojaque (C1) Cali (C1) Cane17 (C1) Capablanca (C1) Caravan (C1) Catera (C1) Chargie21 (C1) CharlieB (C1) ChaylaJr (C1) ChickenPhender (C1) Chomp (C1) CindyLou (C1) Colt (C1) Concombre (C1) Daffodil (C1) Dandelion (C1) Darko (C1) Delilah (C1) Delylah (C1) Demodog (C1) Derek (C1) Dietrick (C1) DirtMonster (C1) Domi (C1) Drazdys (C1) DrPhinkDaddy (C1) DTDevon (C1) EasyJones (C1) EggyFarm (C1) EmmaElysia (C1) EmToTheThree (C1) Erdmann (C1) ErnieJ (C1) Essence (C1) ET08 (C1) Ewok (C1) Flabslab (C1) Fludd (C1) FoxtrotP1 (C1) FrayBell (C1) FudgeTart (C1) Gabriel (C1) Gamgam (C1) Ghost (C1) Gizmo (C1) Grasshills (C1) Grungle (C1) Guwapp (C1) Halldule (C1) HyRo (C1) I3 (C1) IkeLoa (C1) InigoMontoya (C1) InterFolia (C1) Iota (C1) Issimir (C1) Izajani (C1) Janiyra (C1) Jaygup (C1) JayJay (C1) Jedediah (C1) Jemmno (C1) Jessibeth14 (C1) JigglyPuff (C1) JPickles (C1) JulietS (C1) JustHall (C1) Kamryn (C1) Kboogie (C1) Khaleesi (C1) Koguma (C1) Latch (C1) Lee7 (C1) Lethe (C1) LifeSavor (C1) LinStu (C1) Littleton (C1) Loanshark (C1) LolaVinca (C1) LordLeafolot (C1) LRRHood (C1) Lukilu (C1) Maby2021 (C1) Mangeria (C1) Matsumoto (C1) McGee (C1) McWolfish (C1) Megamind (C1) Melpomini (C1) MikeLiesIn (C1) Mikro (C1) Momo (C1) MoMoMixon (C1) Morizzled23 (C1) Naija (C1) Napoleon13 (C1) Nappy (C1) Naval22 (C1) NedWong (C1) Ngannou (C1) Nidhogg (C1) NoodleTree (C1) Norm (C1) NuevoMundo (C1) ParkTD (C1) Patter (C1) Phlegm (C1) Phox (C1) Phusco (C1) Pier (C1) Pinkcreek (C1) Pio (C1) Pivoine (C1) Pleione (C1) PurduePete (C1) QBert (C1) Quasimodo (C1) Rabinovish (C1) Rahel (C1) Rizal (C1) RoMag (C1) Ronan (C1) Roots515 (C1) Salacia (C1) Sauce (C1) ScottMcG (C1) Sebata (C1) Shaqnato (C1) Shelob (C1) ShiaLabeouf (C1) Shifa (C1) Shnickers (C1) Shrimp (C1) SilverDipper (C1) SmallFry (C1) Spec (C1) Specks (C1) Sprinklers (C1) Spud (C1) SrishMeg2525 (C1) StephanieG (C1) Stubby (C1) StubbySaigey (C1) Tainan (C1) Teardrop (C1) TinyTim (C1) Tonenili (C1) Tortoise16 (C1) Trinitium (C1) Turret (C1) Tyke (C1) ValleyTerrace (C1) Wally (C1) Willis (C1) Yassified (C1) YemiJoy2021 (C1) YoungMoneyMata (C1) Yucca (C1) Zalkecks (C1) Zeenon (C1) ZygoTaiga (C1) Greely (C2) Myrna (C2) Phabba (C2) ScoobyDoobyDoo (C2)
Annotating Institution	University of California, Los Angeles
Annotation Status	In GenBank
Plaque Notes	Plaque morphology: clear Average sizesize range diameter: 1.5 - 2 mm
Morphotype	Myoviridae
Number of Genes	216
Number of tRNAs	31
Number of tmRNAs	1
Has been Phamerated?	Yes
Gene List	Click to View Grungle_1 Grungle_2 Grungle_3 (nucleotidyltransferase) Grungle_4 Grungle_5 Grungle_6 Grungle_7 Grungle_8 Grungle_9 Grungle_10 Grungle_11 Grungle_12 Grungle_13 Grungle_14 Grungle_15 Grungle_16 Grungle_17 Grungle_18 Grungle_19 Grungle_20 Grungle_21 Grungle_22 (band-7-like membrane protein) Grungle_23 Grungle_24 Grungle_25 Grungle_26 Grungle_27 Grungle_28 Grungle_29 Grungle_30 Grungle_31 Grungle_32 Grungle_33 Grungle_34 Grungle_35 Grungle_36 Grungle_37 Grungle_38 Grungle_39 (immunity repressor) Grungle_40 Grungle_41 Grungle_42 Grungle_43 Grungle_44 Grungle_45 Grungle_46 Grungle_47 Grungle_48 Grungle_49 Grungle_50 Grungle_51 Grungle_52 Grungle_53 Grungle_54 (helix-turn-helix DNA binding domain protein) Grungle_55 (helix-turn-helix DNA binding protein) Grungle_56 Grungle_57 Grungle_58 Grungle_59 Grungle_60 Grungle_61 Grungle_62 Grungle_63 Grungle_64 Grungle_65 Grungle_66 Grungle_67 Grungle_68 Grungle_69 Grungle_70 Grungle_71 Grungle_72 — tRNA (codon recognized: AGC) Grungle_73 — tRNA (codon recognized: CUG) Grungle_74 — tRNA (codon recognized: CUC) Grungle_75 Grungle_76 Grungle_77 (portal protein) Grungle_78 Grungle_79 Grungle_80 (ThyX-like thymidylate synthase) Grungle_81 Grungle_82 (RNA ligase) Grungle_83 (HNH endonuclease) Grungle_84 Grungle_85 Grungle_86 Grungle_87 (major capsid protein) Grungle_88 (holliday junction resolvase) Grungle_89 Grungle_90 Grungle_91 Grungle_92 Grungle_93 Grungle_94 Grungle_95 Grungle_96 Grungle_97 Grungle_98 Grungle_99 Grungle_100 Grungle_101 Grungle_102 Grungle_103 Grungle_104 Grungle_105 Grungle_106 Grungle_107 Grungle_108 Grungle_109 Grungle_110 Grungle_111 Grungle_112 Grungle_113 Grungle_114 (tail sheath protein) Grungle_115 Grungle_116 Grungle_117 (tail assembly chaperone) Grungle_118 (tail assembly chaperone) Grungle_119 (tape measure protein) Grungle_120 Grungle_121 Grungle_122 Grungle_123 (minor tail protein) Grungle_124 Grungle_125 (minor tail protein) Grungle_126 (baseplate wedge protein) Grungle_127 (baseplate J protein) Grungle_128 Grungle_129 Grungle_130 Grungle_131 (minor tail protein) Grungle_132 (minor tail protein) Grungle_133 Grungle_134 Grungle_135 Grungle_136 Grungle_137 Grungle_138 Grungle_139 Grungle_140 — tRNA (codon recognized: CCA) Grungle_141 — tRNA (codon recognized: UGG) Grungle_142 Grungle_143 (peptidyl tRNA hydrolase) Grungle_144 — tRNA Grungle_145 — tRNA (codon recognized: AUG) Grungle_146 — tRNA (codon recognized: UGC) Grungle_147 — tRNA (codon recognized: GAG) Grungle_148 — tRNA (codon recognized: CAC) Grungle_149 Grungle_150 — tRNA (codon recognized: GCA) Grungle_151 — tRNA (codon recognized: UUC) Grungle_152 — tRNA (codon recognized: GUG) Grungle_153 — tRNA (codon recognized: AAG) Grungle_154 — tRNA (codon recognized: GAA) Grungle_155 — tRNA (codon recognized: GGA) Grungle_156 — tRNA (codon recognized: ACG) Grungle_157 — tRNA (codon recognized: ACA) Grungle_158 — tRNA (codon recognized: ACC) Grungle_159 (minor tail protein) Grungle_160 Grungle_161 — tRNA (codon recognized: GGC) Grungle_162 — tRNA (codon recognized: GAC) Grungle_163 — tRNA (codon recognized: AUG) Grungle_164 — tRNA (codon recognized: AUC) Grungle_165 — tRNA (codon recognized: CGU) Grungle_166 — tRNA (codon recognized: GUC) Grungle_167 — tRNA (codon recognized: AGG) Grungle_168 — tmRNA (tmRNA) Grungle_169 — tRNA (codon recognized: CAA) Grungle_170 — tRNA (codon recognized: AGA) Grungle_171 — tRNA (codon recognized: AAA) Grungle_172 Grungle_173 (DNA helicase) Grungle_174 Grungle_175 Grungle_176 Grungle_177 Grungle_178 Grungle_179 Grungle_180 Grungle_181 Grungle_182 (thymidylate kinase) Grungle_183 (aminotransferase) Grungle_184 Grungle_185 Grungle_186 (DnaC-like helicase loader) Grungle_187 (DnaB-like dsDNA helicase) Grungle_188 Grungle_189 (DNA primase) Grungle_190 Grungle_191 (DnaJ-like chaperonin) Grungle_192 Grungle_193 (DnaE-like DNA polymerase III) Grungle_194 (RF-1 peptide chain release factor) Grungle_195 (RecA-like DNA recombinase) Grungle_196 (holliday junction resolvase) Grungle_197 Grungle_198 (HNH endonuclease) Grungle_199 (RusA-like resolvase) Grungle_200 Grungle_201 Grungle_202 Grungle_203 Grungle_204 Grungle_205 Grungle_206 Grungle_207 — tRNA (codon recognized: CAG) Grungle_208 — tRNA (codon recognized: AAC) Grungle_209 Grungle_210 Grungle_211 Grungle_212 Grungle_213 Grungle_214 Grungle_215 (Ro-like RNA binding protein) Grungle_216 Grungle_217 Grungle_218 Grungle_219 Grungle_220 Grungle_221 Grungle_222 (glycosyltransferase) Grungle_223 Grungle_224 (glycosyltransferase) Grungle_225 (glycosyltransferase) Grungle_226 (PnuC-like nicotinamide riboside transporter) Grungle_227 (glycosyltransferase) Grungle_228 Grungle_229 Grungle_230 Grungle_231 Grungle_232 (lysin A) Grungle_233 (holin) Grungle_234 (lysin B) Grungle_235 (terminase) Grungle_236 Grungle_237 Grungle_238 Grungle_239 Grungle_240 (polynucleotide kinase) Grungle_241 Grungle_242 Grungle_243 (serine/threonine kinase) Grungle_244 Grungle_245 Grungle_246 (PurA-like adenylosuccinate synthetase) Grungle_247 Grungle_248
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MN062707
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	78
Pitt Freezer Box Grid#	C4
Available Files
Plaque Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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