| Detailed Information for Phage HyRo |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Hylton Rodel |
| Year Found | 2014 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.811771 S, 30.916203 E Map |
| Discovery Notes | Soil Sample obtained from very dry, dusty soil located in the middle of a dirt foot path, likely being formed due to high traffic of dogs on the property. Sample obtained in winter (particularly dry season at isolation locale). Lysate pure and in third round of purification. |
| Naming Notes | First two letters of the first and last names of the discoverer |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 18, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1140 |
| Genome length (bp) | 153714 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | No immunity displayed on any lysogen, Che9D had no visible plaque, however poor bacterial lawn growth was observed on Che9D plate. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are circular, clear and approximately 1 mm in diameter at 24h of incubation, growing only slightly larger upon further incubation, reaching approximately 1.75 - 2mm after 48h incubation. Plaque picture was taken after 24h incubation. |
| Morphotype | Myoviridae |
| Number of Genes | 224 |
| Number of tRNAs | 26 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT281790 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
F8 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
