| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | A. Beach, J. Angeles, S. Kushner, T. Gray |
| Year Found | 2021 |
| Location Found | Longview, TX United States |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.465224 N, 94.726598 W Map |
| Discovery Notes | The weather outside for the discovery day was cloudy with a 50% chance of rain. The high that day was 33.9°C and the low was 23.9°C. We found Ikeloa underneath rich, moist, black mulch around bushes. In the surrounding area were worms and lots of organismal life. |
| Naming Notes | The SEA-PHAGES program that our school did was an incredible learning experience and we could not be more grateful for the opportunities to take part in such valuable research. The Hawaiian term "'Ike Loa" refers to the value of learning and knowledge, of growth and expanding your mind. We thought this beautiful phrase perfectly encapsulated our journey of phage-hunting and how appreciative we are to school and God that we may learn and grow in truth and knowledge. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 1, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 233 |
| Genome length (bp) | 155280 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | LeTourneau University |
| Annotation Status | In GenBank |
| Plaque Notes | These plaques were very small in diameter and relatively cloudy. There were no halos, but generally uniform in shape across the entire plate. After taking the average of 10 plaque diameters, we reached an average plaque diameter of 0.7 mm. |
| Morphotype | Myoviridae |
| Number of Genes | 229 |
| Number of tRNAs | 32 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP021682 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
134 |
| Pitt Freezer Box Grid# |
D2 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
