| Detailed Information for Phage JPickles |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Leya Givvines |
| Year Found | 2018 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 39.99479 N, 75.243011 W Map |
| Discovery Notes | -Collected at 11:14 am on September 4th 2018
-Collected near Saint Joseph's University Chapel
-Soil Slightly moist at collection
-Soil was collected 5.1 cm down
-Soil collected while outside temperature was 32.78 degrees Celsius
-Humidity was 50% at time of collection
-There was moderate temperature days before collection. |
| Naming Notes | Pickles is a code word that my boyfriend and I use and his name starts with a J, therefore JPickles. Name was changed from Pickles1025 to JPickles after second titration for simplicity. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 25, 2019 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1221 |
| Genome length (bp) | 155116 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | Phage produced clear plaques ranging from 2 to 4 mm in diameter. Plaques developed after two days in incubator at 37 degrees Celsius. |
| Morphotype | Myoviridae |
| Number of Genes | 230 |
| Number of tRNAs | 33 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN428066 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
85 |
| Pitt Freezer Box Grid# |
D12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |