| Detailed Information for Phage Loanshark |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Brandon Reed |
| Year Found | 2015 |
| Location Found | Lilburn, GA USA |
| Finding Institution | Georgia Gwinnett College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.869367 N, 84.063531 W Map |
| Discovery Notes | Was sunny, near a garden and how slight shade.
88 degrees F
dug 1 inch in to the surface.
Was named because sharks are my favorite species of animals and needed to combine in with something, so I just picked loan since it was the first thing to come to mind. |
| Naming Notes | Sharks are my favorite animal. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 672 |
| Genome length (bp) | 154593 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.8% |
| Sequencing Notes | at position 101361 43% of reads have a deletion instead of consensus G |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Lytic, 1mm circumference |
| Morphotype | Myoviridae |
| Number of Genes | 231 |
| Number of tRNAs | 32 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK359365 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2,38x10^10 PFU/ml |
| Date of SEA Lysate Titering |
Nov 18, 2015 |
| Pitt Freezer Box# |
25 |
| Pitt Freezer Box Grid# |
A12 |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |