| Detailed Information for Phage LolaVinca |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Denisse L. Hasan |
| Year Found | 2018 |
| Location Found | Vero Beach, FL USA |
| Finding Institution | Indian River State College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 27.601687 N, 80.420559 W Map |
| Discovery Notes | Date/Time collected: Sep 2018 at 13:04
Temperature: 30° C (86° F)
Location: Soil obtained from outdoor residential flower garden containing Vinca flowers
Soil: dry, brown, contained spores from Leucocoprinus birnbaumii growing near base of plants |
| Naming Notes | Soil sample was collected from my grandmother's (Lola) flower garden, containing multi-color Catharanthus roseus (vinca). |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 3, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 367 |
| Genome length (bp) | 156412 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Indian River State College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are circular, clear zones in various sizes, most are approximately 1 mm in diameter. |
| Morphotype | Myoviridae |
| Number of Genes | 232 |
| Number of tRNAs | 29 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN183280 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
77 |
| Pitt Freezer Box Grid# |
H12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
