| Detailed Information for Phage McWolfish |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Lysocool |
| Found By | Lucas McClish & Tony Wolfe |
| Year Found | 2014 |
| Location Found | Columbus, OH USA |
| Finding Institution | The Ohio State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 39.999508 N, 83.012663 W Map |
| Discovery Notes | Enrichment plates were obtained from other classmates cultures due to lack of phage from soil sample. Agarose gel electrophoresis produced what appeared to be unique DNA. |
| Naming Notes | It's a play of words off of lysosomes. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 719 |
| Genome length (bp) | 154462 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Was mostly lytic morphology with plaques about the size of an eraser head. For final dilution, 10^-5 appeared half lytic, half temperate; 10^-4 however appeared purely lytic. |
| Morphotype | Myoviridae |
| Number of Genes | 230 |
| Number of tRNAs | 33 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK112548 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.06x10^14 pfu/mL |
| Date of SEA Lysate Titering |
Oct 29, 2014 |
| Pitt Freezer Box# |
12 |
| Pitt Freezer Box Grid# |
B8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
