| Detailed Information for Phage Melpomini |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Yevgeniya Miglo |
| Year Found | 2014 |
| Location Found | Baltimore, MD USA |
| Finding Institution | Johns Hopkins University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.328399 N, 76.618048 W Map |
| Discovery Notes | Obtained: 8/27/2014 2:20pm 88 degrees f
3 cm in depth
dry
urban
across the street from buildings
foot traffic about 4 meters away
Charles Street about 5 meters away
under a tree - indirect sunlight
on a slope |
| Naming Notes | Named after Melpomene, after the greek muse of tragedy.
Because of the small tail, "mene" was changed to "mini." |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 18, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1658 |
| Genome length (bp) | 154855 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | 1-2 mm clear plaques at 48 hours |
| Morphotype | Myoviridae |
| Number of Genes | 228 |
| Number of tRNAs | 32 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK450424 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.8 x 10^11 pfu/mL |
| Date of SEA Lysate Titering |
Oct 23, 2014 |
| Pitt Freezer Box# |
14 |
| Pitt Freezer Box Grid# |
A10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
