Mycobacterium phage Mikro
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Detailed Information for Phage Mikro
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByVictoria Frost
Year Found2021
Location FoundRock Hill, SC United States of America
Finding InstitutionWinthrop University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates34.940074 N, 81.033099 W Map
Discovery NotesSoil sample was collected under flower bed located at the Little Chapel on the Winthrop University campus.
Naming NotesI wanted to name it after a gemstone from my favorite TV show, and my plaques/phages have been quite small so far. No gems in the show were that small, so I decided to find the name of the world's smallest gem.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 3, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1503
Genome length (bp)157166
Character of genome endsCircularly Permuted
GC Content64.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionWinthrop University
Annotation StatusIn GenBank
Plaque NotesPlaques were always very tiny, about 3mm.
MorphotypeMyoviridae
Number of Genes228
Number of tRNAs32
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Mikro_1

Mikro_2

Mikro_3
(nucleotidyltransferase)

Mikro_4

Mikro_5

Mikro_6

Mikro_7

Mikro_8

Mikro_9

Mikro_10

Mikro_11

Mikro_12

Mikro_13

Mikro_14

Mikro_15

Mikro_16

Mikro_17

Mikro_18

Mikro_19

Mikro_20

Mikro_21

Mikro_22

Mikro_23
(HNH endonuclease)

Mikro_24

Mikro_25

Mikro_26

Mikro_27
(band-7-like membrane protein)

Mikro_28

Mikro_29
(helix-turn-helix DNA binding domain protein)

Mikro_30

Mikro_31

Mikro_32

Mikro_33
(ribbon-helix-helix DNA binding domain protein)

Mikro_34
(DNA binding protein)

Mikro_35

Mikro_36

Mikro_37

Mikro_38

Mikro_39

Mikro_40

Mikro_41

Mikro_42

Mikro_43

Mikro_44

Mikro_45

Mikro_46

Mikro_47

Mikro_48

Mikro_49

Mikro_50

Mikro_51

Mikro_52

Mikro_53

Mikro_54

Mikro_55

Mikro_56

Mikro_57

Mikro_58

Mikro_59

Mikro_60

Mikro_61
(helix-turn-helix DNA binding domain protein)

Mikro_62
(helix-turn-helix DNA binding domain protein)

Mikro_63

Mikro_64

Mikro_65

Mikro_66

Mikro_67

Mikro_68

Mikro_69

Mikro_70

Mikro_71

Mikro_72

Mikro_73

Mikro_74

Mikro_75

Mikro_76

Mikro_77

Mikro_78

Mikro_79

Mikro_80

Mikro_81

Mikro_82 — tRNA
(tRNA-Ser(gct))

Mikro_83 — tRNA
(tRNA-Leu(cag))

Mikro_84 — tRNA
(tRNA-Leu(gag))

Mikro_85 — tRNA
(tRNA-Leu(caa))

Mikro_86
(methyltransferase)

Mikro_87

Mikro_88
(portal protein)

Mikro_89

Mikro_90

Mikro_91
(ThyX-like thymidylate synthase)

Mikro_92

Mikro_93

Mikro_94

Mikro_95

Mikro_96

Mikro_97
(major capsid protein)

Mikro_98
(holliday junction resolvase)

Mikro_99

Mikro_100

Mikro_101

Mikro_102

Mikro_103

Mikro_104

Mikro_105

Mikro_106

Mikro_107

Mikro_108

Mikro_109

Mikro_110

Mikro_111

Mikro_112

Mikro_113

Mikro_114

Mikro_115

Mikro_116

Mikro_117

Mikro_118
(acetyltransferase)

Mikro_119

Mikro_120

Mikro_121

Mikro_122

Mikro_123

Mikro_124
(tail sheath protein)

Mikro_125

Mikro_126

Mikro_127
(tail assembly chaperone)

Mikro_128
(tail assembly chaperone)

Mikro_129
(tape measure protein)

Mikro_130
(minor tail protein)

Mikro_131

Mikro_132

Mikro_133
(minor tail protein)

Mikro_134

Mikro_135
(minor tail protein)

Mikro_136
(baseplate wedge protein)

Mikro_137
(baseplate wedge protein)

Mikro_138

Mikro_139

Mikro_140

Mikro_141
(minor tail protein)

Mikro_142
(minor tail protein)

Mikro_143

Mikro_144

Mikro_145

Mikro_146

Mikro_147

Mikro_148

Mikro_149

Mikro_150 — tRNA
(tRNA-Pro(tgg))

Mikro_151 — tRNA
(tRNA-Trp(cca))

Mikro_152 — tRNA
(tRNA-Tyr(gta))

Mikro_153

Mikro_154
(peptidyl tRNA hydrolase)

Mikro_155 — tRNA
(tRNA-Pyl (cta))

Mikro_156 — tRNA
(tRNA-Met(cat))

Mikro_157 — tRNA
(tRNA-Cys(gca))

Mikro_158 — tRNA
(tRNA-Glu(ctc))

Mikro_159 — tRNA
(tRNA-His(gtg))

Mikro_160 — tRNA
(tRNA-Ala(tgc))

Mikro_161

Mikro_162 — tRNA
(tRNA-Phe(gaa))

Mikro_163 — tRNA
(tRNA-Val(cac))

Mikro_164 — tRNA
(tRNA-Lys(ctt))

Mikro_165 — tRNA
(tRNA-Glu(ttc))

Mikro_166 — tRNA
(tRNA-Gly(tcc))

Mikro_167 — tRNA
(tRNA-Thr(cgt))

Mikro_168 — tRNA
(tRNA-Thr(tgt))

Mikro_169
(HNH endonuclease)

Mikro_170 — tRNA
(tRNA-Thr(ggt))

Mikro_171
(minor tail protein)

Mikro_172

Mikro_173 — tRNA
(tRNA-Gly(gcc))

Mikro_174 — tRNA
(tRNA-Asp(gtc))

Mikro_175 — tRNA
(tRNA-Met(cat))

Mikro_176 — tRNA
(tRNA-Ile(gat))

Mikro_177 — tRNA
(tRNA-Arg(acg))

Mikro_178 — tRNA
(tRNA-Val(gac))

Mikro_179 — tRNA
(tRNA-Arg(cct))

Mikro_180 — tRNA
(tRNA-Gln(ttg))

Mikro_181 — tRNA
(tRNA-Arg(tct))

Mikro_182

Mikro_183
(DNA helicase)

Mikro_184

Mikro_185

Mikro_186

Mikro_187

Mikro_188

Mikro_189

Mikro_190

Mikro_191

Mikro_192

Mikro_193
(thymidylate kinase)

Mikro_194
(aminotransferase)

Mikro_195

Mikro_196

Mikro_197

Mikro_198
(DnaC-like helicase loader)

Mikro_199
(DnaB-like dsDNA helicase)

Mikro_200

Mikro_201
(DNA primase)

Mikro_202

Mikro_203
(DnaJ-like chaperonin)

Mikro_204
(ssDNA binding protein)

Mikro_205
(DnaE-like DNA polymerase III alpha)

Mikro_206
(RF-1 peptide chain release factor)

Mikro_207
(RecA-like DNA recombinase)

Mikro_208
(holliday junction resolvase)

Mikro_209

Mikro_210
(HNH endonuclease)

Mikro_211
(RusA-like resolvase)

Mikro_212

Mikro_213

Mikro_214

Mikro_215

Mikro_216

Mikro_217

Mikro_218

Mikro_219 — tRNA
(tRNA-Gln(ctg))

Mikro_220 — tRNA
(tRNA-Asn(gtt))

Mikro_221

Mikro_222

Mikro_223

Mikro_224

Mikro_225

Mikro_226

Mikro_227
(Ro-like RNA binding protein)

Mikro_228

Mikro_229

Mikro_230

Mikro_231

Mikro_232

Mikro_233

Mikro_234
(glycosyltransferase)

Mikro_235

Mikro_236
(glycosyltransferase)

Mikro_237
(glycosyltransferase)

Mikro_238
(PnuC-like nicotinamide riboside transporter)

Mikro_239
(glycosyltransferase)

Mikro_240

Mikro_241

Mikro_242

Mikro_243

Mikro_244
(lysin A)

Mikro_245
(holin)

Mikro_246
(lysin B)

Mikro_247
(terminase)

Mikro_248

Mikro_249

Mikro_250

Mikro_251

Mikro_252
(polynucleotide kinase)

Mikro_253

Mikro_254

Mikro_255
(serine/threonine kinase)

Mikro_256

Mikro_257

Mikro_258
(PurA-like adenylosuccinate synthetase)

Mikro_259

Mikro_260

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON456344
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 132
Pitt Freezer Box Grid# G10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload