| Detailed Information for Phage Patter |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jenna Kwiecinski |
| Year Found | 2016 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.94472 N, 75.20778 W Map |
| Discovery Notes | Sample was collected on September 15, 2016, from the A level soil horizon. It was collected from a rain purification area at University of the Sciences. Type of soil was sandy loam according to a ribbon test. Weather was 80 degrees Fahrenheit with cloudless skies and no wind. |
| Naming Notes | The name Patter came from the "pitter-patter" sound that rain makes. My phage was collected from a rain purification area, so I wanted its name to relate to rain/water. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 18, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 591 |
| Genome length (bp) | 155470 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were 1 mm in diameter and lytic. |
| Morphotype | Myoviridae |
| Number of Genes | 235 |
| Number of tRNAs | 33 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK359335 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.11 x 10^9 pfu |
| Date of SEA Lysate Titering |
Oct 11, 2016 |
| Pitt Freezer Box# |
34 |
| Pitt Freezer Box Grid# |
F1 |
| Available Files |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |