The Actinobacteriophage Database | Phage PharkleMinkus

Mycobacterium phage PharkleMinkus

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Detailed Information for Phage PharkleMinkus
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Nazifa Chowdhury and Deeya Patel
Year Found	2025
Location Found	Midlothian, VA United States
Finding Institution	Virginia Commonwealth University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	37.499765 N, 77.671381 W Map
Discovery Notes	Soil Sample: Collected near the surface from a potted Jasminum sambac (jasmine plant) Weather: Partly Sunny Soil Observations: Mulchy and somewhat moist, in an area that sees human activity daily, could possibly infested with small animals, contains soil from Lowe’s and non-natural nutrients (store bot nutrients).
Naming Notes	Girl Meets World- Farkle Minkus
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 9, 2026
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina NextSeq 1000
Read Type	Single-end reads
Read Length	100 bp
Approximate Shotgun Coverage	273
Genome length (bp)	154863
Character of genome ends	Circularly Permuted
GC Content	64.7%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	C
Subcluster	C1
Cluster Life Cycle	Lytic
Lysogeny Notes	TEM imaging shows a Myoviridae structure, which is known for large genomes and lytic life cycles. Our gels also revealed a strong high-molecular-weight DNA band, which fits that expectation. In addition, the Phage Release Assay showed no plaques and full bacterial growth, suggesting that Pharkle Minkus kills its host instead of integrating. No phage is released, so it is presumed lytic.
Other Cluster Members	Click to View Ablatia (C1) Ading (C1) Adlitam (C1) Alice (C1) Amataga (C1) ArcherS7 (C1) Asterius (C1) Astraea (C1) Atlantean (C1) Audrick (C1) Ava3 (C1) Babyland (C1) BackyardAgain (C1) BadAgartude (C1) BananaFence (C1) Bangla1971 (C1) Basquiat (C1) BeanWater (C1) Belieber (C1) Bezza (C1) BigCity (C1) Bigswole (C1) Bipolarisk (C1) Blackbrain (C1) Blackdragon (C1) Blubba (C1) BobaPhatt (C1) Bonray (C1) Bowman (C1) Bread (C1) Breeniome (C1) Burrough (C1) Bxz1 (C1) Cactojaque (C1) Cali (C1) Cane17 (C1) Capablanca (C1) Caravan (C1) Catera (C1) Chargie21 (C1) CharlieB (C1) ChaylaJr (C1) ChickenPhender (C1) Chomp (C1) CindyLou (C1) Colt (C1) Concombre (C1) Daffodil (C1) Dandelion (C1) Darko (C1) DeejaClivia (C1) Delilah (C1) Delylah (C1) Demodog (C1) Derek (C1) Dessert (C1) Dietrick (C1) DirtMonster (C1) Domi (C1) Drazdys (C1) DrPhinkDaddy (C1) DTDevon (C1) Duckfeet (C1) EasyJones (C1) EggyFarm (C1) EmmaElysia (C1) EmToTheThree (C1) Erdmann (C1) ErnieJ (C1) Essence (C1) ET08 (C1) Ewok (C1) Fixie (C1) Flabslab (C1) Fludd (C1) Fongie (C1) FoxtrotP1 (C1) FrayBell (C1) FudgeTart (C1) Gabriel (C1) Gamgam (C1) Ghost (C1) Gizmo (C1) GlutenPhree (C1) Grasshills (C1) Grungle (C1) Guwapp (C1) Halldule (C1) HyRo (C1) I3 (C1) IkeLoa (C1) InigoMontoya (C1) InterFolia (C1) Iota (C1) Issimir (C1) Izajani (C1) Janiyra (C1) Jaygup (C1) JayJay (C1) Jedediah (C1) Jemmno (C1) Jessibeth14 (C1) Jezreel (C1) JigglyPuff (C1) JPickles (C1) JulietS (C1) JustHall (C1) Kamryn (C1) Kboogie (C1) Khaleesi (C1) Koguma (C1) Labradorite (C1) Latch (C1) Lee7 (C1) Lethe (C1) LifeSavor (C1) LinStu (C1) Littleton (C1) Loanshark (C1) LolaVinca (C1) LookingGlass (C1) LordLeafolot (C1) LRRHood (C1) Lukilu (C1) Lunareta (C1) Maby2021 (C1) Mangeria (C1) Matsumoto (C1) McGee (C1) McWolfish (C1) Megamind (C1) Melpomini (C1) MikeLiesIn (C1) Mikro (C1) Moby (C1) Moiqah (C1) Momo (C1) MoMoMixon (C1) Montpel (C1) Morizzled23 (C1) Naija (C1) Napoleon13 (C1) Nappy (C1) Naval22 (C1) NedWong (C1) Ngannou (C1) Nidhogg (C1) NoodleTree (C1) Norm (C1) NuevoMundo (C1) OrediggerDelux (C1) ParkTD (C1) Patter (C1) PharkleMinkus (C1) Phlegm (C1) Phox (C1) Phusco (C1) Pier (C1) Pinkcreek (C1) Pio (C1) Pivoine (C1) Pleione (C1) Primadonna (C1) Pupusa (C1) PurduePete (C1) QBert (C1) Quasimodo (C1) Rabinovish (C1) Rahel (C1) Rizal (C1) Rocha (C1) RoMag (C1) Ronan (C1) Roots515 (C1) Salacia (C1) Sauce (C1) ScottMcG (C1) Sebata (C1) Shaqnato (C1) Shelob (C1) ShiaLabeouf (C1) Shifa (C1) Shnickers (C1) Shrimp (C1) SilverDipper (C1) SmallFry (C1) Spec (C1) Specks (C1) Sprinklers (C1) Spud (C1) SrishMeg2525 (C1) StephanieG (C1) Stubby (C1) StubbySaigey (C1) SVoro (C1) Tainan (C1) Teardrop (C1) TinyTim (C1) Tonenili (C1) Tortoise16 (C1) Trinitium (C1) Turret (C1) Tyke (C1) ValleyTerrace (C1) Vortrix (C1) Wally (C1) Willis (C1) Yassified (C1) YemiJoy2021 (C1) YoungMoneyMata (C1) Yucca (C1) Zalkecks (C1) Zeenon (C1) ZygoTaiga (C1) Greely (C2) Myrna (C2) Phabba (C2) ScoobyDoobyDoo (C2)
Annotating Institution	Virginia Commonwealth University
Annotation Status	Being Annotated (Expected completion by 5/1/2026)
Plaque Notes	Similar shape: Tiny irregular shaped circles Similar size: Roughly 0.08 - 0.1 cm long and tall Physical Observation: Clear all through the shape; not turbid or halo shaped
Morphotype	Myoviridae
Number of Genes	237
Number of tRNAs	33
Number of tmRNAs	1
Has been Phamerated?	Yes
Gene List	Click to View PharkleMinkus_1 PharkleMinkus_2 PharkleMinkus_3 PharkleMinkus_4 PharkleMinkus_5 PharkleMinkus_6 PharkleMinkus_7 PharkleMinkus_8 PharkleMinkus_9 PharkleMinkus_10 PharkleMinkus_11 PharkleMinkus_12 PharkleMinkus_13 PharkleMinkus_14 PharkleMinkus_15 PharkleMinkus_16 PharkleMinkus_17 PharkleMinkus_18 PharkleMinkus_19 PharkleMinkus_20 PharkleMinkus_21 PharkleMinkus_22 PharkleMinkus_23 PharkleMinkus_24 PharkleMinkus_25 PharkleMinkus_26 PharkleMinkus_27 PharkleMinkus_28 PharkleMinkus_29 PharkleMinkus_30 PharkleMinkus_31 PharkleMinkus_32 PharkleMinkus_33 PharkleMinkus_34 PharkleMinkus_35 PharkleMinkus_36 PharkleMinkus_37 PharkleMinkus_38 PharkleMinkus_39 PharkleMinkus_40 PharkleMinkus_41 PharkleMinkus_42 PharkleMinkus_43 PharkleMinkus_44 PharkleMinkus_45 PharkleMinkus_46 PharkleMinkus_47 PharkleMinkus_48 PharkleMinkus_49 PharkleMinkus_50 PharkleMinkus_51 PharkleMinkus_52 PharkleMinkus_53 PharkleMinkus_54 PharkleMinkus_55 PharkleMinkus_56 PharkleMinkus_57 PharkleMinkus_58 PharkleMinkus_59 PharkleMinkus_60 PharkleMinkus_61 PharkleMinkus_62 PharkleMinkus_63 PharkleMinkus_64 PharkleMinkus_65 PharkleMinkus_66 PharkleMinkus_67 PharkleMinkus_68 PharkleMinkus_69 PharkleMinkus_70 PharkleMinkus_71 PharkleMinkus_72 PharkleMinkus_73 PharkleMinkus_74 PharkleMinkus_75 PharkleMinkus_76 PharkleMinkus_77 PharkleMinkus_78 PharkleMinkus_79 PharkleMinkus_80 PharkleMinkus_81 PharkleMinkus_82 PharkleMinkus_83 PharkleMinkus_84 PharkleMinkus_85 PharkleMinkus_86 PharkleMinkus_87 PharkleMinkus_88 PharkleMinkus_89 — tRNA (gp89) PharkleMinkus_90 — tRNA (gp90) PharkleMinkus_91 — tRNA (gp91) PharkleMinkus_92 — tRNA (gp92) PharkleMinkus_93 PharkleMinkus_94 PharkleMinkus_95 PharkleMinkus_96 PharkleMinkus_97 PharkleMinkus_98 PharkleMinkus_99 PharkleMinkus_100 PharkleMinkus_101 PharkleMinkus_102 PharkleMinkus_103 PharkleMinkus_104 PharkleMinkus_105 PharkleMinkus_106 PharkleMinkus_107 PharkleMinkus_108 PharkleMinkus_109 PharkleMinkus_110 PharkleMinkus_111 PharkleMinkus_112 PharkleMinkus_113 PharkleMinkus_114 PharkleMinkus_115 PharkleMinkus_116 PharkleMinkus_117 PharkleMinkus_118 PharkleMinkus_119 PharkleMinkus_120 PharkleMinkus_121 PharkleMinkus_122 PharkleMinkus_123 PharkleMinkus_124 PharkleMinkus_125 PharkleMinkus_126 PharkleMinkus_127 PharkleMinkus_128 PharkleMinkus_129 PharkleMinkus_130 PharkleMinkus_131 — tRNA (gp131) PharkleMinkus_132 PharkleMinkus_133 PharkleMinkus_134 PharkleMinkus_135 PharkleMinkus_136 PharkleMinkus_137 PharkleMinkus_138 PharkleMinkus_139 PharkleMinkus_140 PharkleMinkus_141 PharkleMinkus_142 PharkleMinkus_143 PharkleMinkus_144 PharkleMinkus_145 PharkleMinkus_146 PharkleMinkus_147 PharkleMinkus_148 PharkleMinkus_149 PharkleMinkus_150 PharkleMinkus_151 PharkleMinkus_152 PharkleMinkus_153 PharkleMinkus_154 PharkleMinkus_155 PharkleMinkus_156 PharkleMinkus_157 — tRNA (gp157) PharkleMinkus_158 — tRNA (gp158) PharkleMinkus_159 — tRNA (gp159) PharkleMinkus_160 PharkleMinkus_161 PharkleMinkus_162 — tRNA (gp162) PharkleMinkus_163 — tRNA (gp163) PharkleMinkus_164 — tRNA (gp164) PharkleMinkus_165 — tRNA (gp165) PharkleMinkus_166 — tRNA (gp166) PharkleMinkus_167 — tRNA (gp167) PharkleMinkus_168 PharkleMinkus_169 — tRNA (gp169) PharkleMinkus_170 — tRNA (gp170) PharkleMinkus_171 — tRNA (gp171) PharkleMinkus_172 — tRNA (gp172) PharkleMinkus_173 — tRNA (gp173) PharkleMinkus_174 — tRNA (gp174) PharkleMinkus_175 — tRNA (gp175) PharkleMinkus_176 — tRNA (gp176) PharkleMinkus_177 PharkleMinkus_178 PharkleMinkus_179 — tRNA (gp179) PharkleMinkus_180 — tRNA (gp180) PharkleMinkus_181 — tRNA (gp181) PharkleMinkus_182 — tRNA (gp182) PharkleMinkus_183 — tRNA (gp183) PharkleMinkus_184 — tRNA (gp184) PharkleMinkus_185 — tRNA (gp185) PharkleMinkus_186 — tmRNA (gp186) PharkleMinkus_187 — tRNA (gp187) PharkleMinkus_188 — tRNA (gp188) PharkleMinkus_189 PharkleMinkus_190 PharkleMinkus_191 PharkleMinkus_192 PharkleMinkus_193 PharkleMinkus_194 PharkleMinkus_195 PharkleMinkus_196 PharkleMinkus_197 PharkleMinkus_198 PharkleMinkus_199 PharkleMinkus_200 PharkleMinkus_201 PharkleMinkus_202 PharkleMinkus_203 PharkleMinkus_204 PharkleMinkus_205 PharkleMinkus_206 PharkleMinkus_207 PharkleMinkus_208 PharkleMinkus_209 PharkleMinkus_210 PharkleMinkus_211 PharkleMinkus_212 PharkleMinkus_213 PharkleMinkus_214 PharkleMinkus_215 PharkleMinkus_216 PharkleMinkus_217 PharkleMinkus_218 PharkleMinkus_219 PharkleMinkus_220 PharkleMinkus_221 PharkleMinkus_222 PharkleMinkus_223 PharkleMinkus_224 PharkleMinkus_225 — tRNA (gp225) PharkleMinkus_226 — tRNA (gp226) PharkleMinkus_227 PharkleMinkus_228 PharkleMinkus_229 PharkleMinkus_230 PharkleMinkus_231 PharkleMinkus_232 PharkleMinkus_233 PharkleMinkus_234 PharkleMinkus_235 PharkleMinkus_236 PharkleMinkus_237 PharkleMinkus_238 PharkleMinkus_239 PharkleMinkus_240 PharkleMinkus_241 PharkleMinkus_242 PharkleMinkus_243 PharkleMinkus_244 PharkleMinkus_245 PharkleMinkus_246 PharkleMinkus_247 PharkleMinkus_248 PharkleMinkus_249 PharkleMinkus_250 PharkleMinkus_251 PharkleMinkus_252 PharkleMinkus_253 PharkleMinkus_254 PharkleMinkus_255 PharkleMinkus_256 PharkleMinkus_257 PharkleMinkus_258 PharkleMinkus_259 PharkleMinkus_260 PharkleMinkus_261 PharkleMinkus_262 PharkleMinkus_263 PharkleMinkus_264 PharkleMinkus_265 PharkleMinkus_266 PharkleMinkus_267 PharkleMinkus_268 PharkleMinkus_269 PharkleMinkus_270 PharkleMinkus_271
Publication Info
Uploaded to GenBank?	No
GenBank Accession	None yet
Refseq Number	None yet
Archiving Info
Archiving status	Not in Pitt Archives
SEA Lysate Titer	10^8
Available Files
Plaque Picture	Download
EM Picture	Download

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