Mycobacterium phage PharkleMinkus
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| Detailed Information for Phage PharkleMinkus | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nazifa Chowdhury and Deeya Patel |
| Year Found | 2025 |
| Location Found | Midlothian, VA United States |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.499765 N, 77.671381 W Map |
| Discovery Notes | Soil Sample: Collected near the surface from a potted Jasminum sambac (jasmine plant) Weather: Partly Sunny Soil Observations: Mulchy and somewhat moist, in an area that sees human activity daily, could possibly infested with small animals, contains soil from Lowe’s and non-natural nutrients (store bot nutrients). |
| Naming Notes | Girl Meets World- Farkle Minkus |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | TEM imaging shows a Myoviridae structure, which is known for large genomes and lytic life cycles. Our gels also revealed a strong high-molecular-weight DNA band, which fits that expectation. In addition, the Phage Release Assay showed no plaques and full bacterial growth, suggesting that Pharkle Minkus kills its host instead of integrating. No phage is released, so it is presumed lytic. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Similar shape: Tiny irregular shaped circles Similar size: Roughly 0.08 - 0.1 cm long and tall Physical Observation: Clear all through the shape; not turbid or halo shaped |
| Morphotype | Myoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 10^8 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
