Mycobacterium phage Phox
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Detailed Information for Phage Phox
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDavid Parra
Year Found2016
Location FoundWalnut City, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature35°C
GPS Coordinates34.04 N, 117.84 W Map
Discovery NotesPhage Phox was isolated from dry soil collected at a depth of 4cm on 10/2/16 by David Parra. Air temp at time of collection was 18C. Phox was isolated by Direct plating at 35C.

Characterization was perform by Team Plaque Hole (David Parra, Christopher Hsu, Quan Le, Karen Lam) and Team Phage and Phurious (Gregory Rosenberg, Lifan Jin, Jonathan Hwang, Daniel Shen)

Phage Phox was annotated by Team Plaque Hole, Team Phage and Phurious, Team Phaged and Confused (Alexis Doherty, Alexis Dowiak, Regis Fallon, Joshua Green), and Team Phage-A Vu (Marco Aquinde, Jennifer Bergsma, Thuytien Duong, Catherine Le).

Instructor: Dr. Jordan Moberg Parker
Teaching Assistants: Michelle Bina & Andrew Lund
Undergrad Assistant: Samuel Wu
Laboratory Support: Dr. Krisanavane Reddi.
Naming NotesKaren's favorite animal is Fox which pronounces like Phox
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 20, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage402
Genome length (bp)154874
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

Lethe (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes1mm pinpoint plaques
MorphotypeMyoviridae
Number of Genes229
Number of tRNAs38
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Phox_1

Phox_2

Phox_3
(nucleotidyltransferase)

Phox_4

Phox_5

Phox_6

Phox_7

Phox_8

Phox_9

Phox_10

Phox_11

Phox_12

Phox_13

Phox_14

Phox_15

Phox_16

Phox_17

Phox_18

Phox_19

Phox_20

Phox_21

Phox_22

Phox_23

Phox_24

Phox_25

Phox_26

Phox_27

Phox_28
(band-7-like membrane protein)

Phox_29

Phox_30

Phox_31

Phox_32

Phox_33

Phox_34

Phox_35

Phox_36

Phox_37

Phox_38

Phox_39

Phox_40

Phox_41

Phox_42

Phox_43

Phox_44

Phox_45
(immunity repressor)

Phox_46

Phox_47

Phox_48

Phox_49

Phox_50

Phox_51

Phox_52

Phox_53

Phox_54

Phox_55

Phox_56

Phox_57

Phox_58

Phox_59

Phox_60

Phox_61
(helix-turn-helix DNA binding domain protein)

Phox_62
(helix-turn-helix DNA binding protein)

Phox_63

Phox_64

Phox_65

Phox_66

Phox_67

Phox_68

Phox_69

Phox_70

Phox_71

Phox_72

Phox_73

Phox_74

Phox_75

Phox_76

Phox_77

Phox_78

Phox_79

Phox_80 — tRNA
(tRNA-Ser(gct))

Phox_81 — tRNA
(tRNA-Undet(nnn); tRNA-Undet(nnn))

Phox_82 — tRNA
(tRNA-Leu(cag))

Phox_83 — tRNA
(tRNA-Leu(gag))

Phox_84 — tRNA
(tRNA-Leu(caa))

Phox_85

Phox_86

Phox_87
(portal protein)

Phox_88

Phox_89

Phox_90
(ThyX-like thymidylate synthase)

Phox_91

Phox_92
(RNA ligase)

Phox_93

Phox_94

Phox_95

Phox_96
(major capsid protein)

Phox_97

Phox_98

Phox_99

Phox_100

Phox_101

Phox_102

Phox_103

Phox_104

Phox_105

Phox_106

Phox_107

Phox_108

Phox_109

Phox_110

Phox_111

Phox_112

Phox_113

Phox_114

Phox_115

Phox_116

Phox_117

Phox_118

Phox_119

Phox_120

Phox_121

Phox_122

Phox_123

Phox_124
(tail sheath protein)

Phox_125

Phox_126

Phox_127
(tail assembly chaperone)

Phox_128
(tail assembly chaperone)

Phox_129
(tape measure protein)

Phox_130

Phox_131

Phox_132

Phox_133
(minor tail protein)

Phox_134

Phox_135
(minor tail protein)

Phox_136
(baseplate wedge protein)

Phox_137
(baseplate J protein)

Phox_138
(HNH endonuclease)

Phox_139

Phox_140

Phox_141

Phox_142

Phox_143
(minor tail protein)

Phox_144

Phox_145

Phox_146

Phox_147

Phox_148

Phox_149

Phox_150

Phox_151 — tRNA
(tRNA-Pro(tgg))

Phox_152 — tRNA
(tRNA-Trp(cca))

Phox_153 — tRNA
(tRNA-Tyr(gta))

Phox_154

Phox_155
(peptidyl tRNA hydrolase)

Phox_156 — tRNA
(tRNA-Undet(nnn); tRNA-Undet(nnn))

Phox_157 — tRNA
(tRNA-Pyl(cta))

Phox_158 — tRNA
(tRNA-Met(cat))

Phox_159 — tRNA
(tRNA-Cys(gca))

Phox_160 — tRNA
(tRNA-Glu(gtc))

Phox_161 — tRNA
(tRNA-His(gtg))

Phox_162 — tRNA
(tRNA-Ala(tgc))

Phox_163 — tRNA
(tRNA-Met(cat))

Phox_164 — tRNA
(tRNA-Phe(gaa))

Phox_165 — tRNA
(tRNA-Val(cac))

Phox_166 — tRNA
(tRNA-Lys(ctt))

Phox_167 — tRNA
(tRNA-Glu(ttc))

Phox_168 — tRNA
(tRNA-Gly(tcc))

Phox_169 — tRNA
(tRNA-Gly(tcc))

Phox_170 — tRNA
(tRNA-Thr(cgt))

Phox_171 — tRNA
(tRNA-Thr(tgt))

Phox_172 — tRNA
(tRNA-Thr(ggt))

Phox_173
(minor tail protein)

Phox_174

Phox_175 — tRNA
(tRNA-Gly(gcc))

Phox_176 — tRNA
(tRNA-Asp(gtc))

Phox_177 — tRNA
(tRNA-Met(cat))

Phox_178 — tRNA
(tRNa-Ile(gat))

Phox_179 — tRNA
(tRNA-Arg(acg))

Phox_180 — tRNA
(tRNA-Val(gac))

Phox_181 — tRNA
(tRNA-Arg (ccg))

Phox_182 — tRNA
(tRNA-Arg(cct))

Phox_183 — tmRNA
(tmRNA)

Phox_184 — tRNA
(tRNA-Gln(ttg))

Phox_185 — tRNA
(tRNA-Arg(tct))

Phox_186 — tRNA
(tRNA-Lys(ttt))

Phox_187

Phox_188
(DNA helicase)

Phox_189

Phox_190

Phox_191

Phox_192

Phox_193

Phox_194

Phox_195

Phox_196

Phox_197

Phox_198
(thymidylate kinase)

Phox_199
(aminotransferase)

Phox_200

Phox_201

Phox_202

Phox_203
(DnaC-like helicase loader)

Phox_204
(DnaB-like dsDNA helicase)

Phox_205

Phox_206
(DNA primase)

Phox_207

Phox_208
(DnaJ-like chaperonin)

Phox_209

Phox_210
(DnaE-like DNA polymerase III)

Phox_211
(RF-1 peptide chain release factor)

Phox_212
(RecA-like DNA recombinase)

Phox_213
(holliday junction resolvase)

Phox_214

Phox_215
(Cas4 family exonuclease)

Phox_216
(RusA-like resolvase)

Phox_217

Phox_218

Phox_219

Phox_220

Phox_221

Phox_222

Phox_223

Phox_224 — tRNA
(tRNA-Gln(ctg))

Phox_225 — tRNA
(tRNA-Asn(gtt))

Phox_226

Phox_227

Phox_228

Phox_229

Phox_230

Phox_231

Phox_232

Phox_233

Phox_234
(Ro-like RNA binding protein)

Phox_235

Phox_236

Phox_237

Phox_238

Phox_239

Phox_240

Phox_241
(glycosyltransferase)

Phox_242

Phox_243
(glycosyltransferase)

Phox_244
(glycosyltransferase)

Phox_245
(PnuC-like nicotinamide riboside transporter)

Phox_246
(glycosyltransferase)

Phox_247

Phox_248

Phox_249

Phox_250

Phox_251
(lysin A)

Phox_252
(holin)

Phox_253
(lysin B)

Phox_254
(terminase)

Phox_255

Phox_256

Phox_257

Phox_258

Phox_259
(polynucleotide kinase)

Phox_260

Phox_261

Phox_262
(serine/threonine kinase)

Phox_263

Phox_264

Phox_265
(PurA-like adenylosuccinate synthetase)

Phox_266

Phox_267

Phox_268

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919527
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 45
Pitt Freezer Box Grid# D1
Available Files
GenBank File for PhameratorDownload