Mycobacterium phage Phusco
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Detailed Information for Phage Phusco
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGrant Rybnicky
Year Found2013
Location FoundHamilton, NJ USA
Finding InstitutionJames Madison University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.178194 N, 74.630944 W Map
Discovery NotesSoil collected from the Richard Stockton rest stop on the NJ Turnpike. The sample was collected near the side walk under the shade of a tree. Came from the same soil sample as the mycobacteriophage Turnpike.
Naming NotesThe name is derived from the last name of my sophmore year Biotechnology teacher in high school. His name is David Fusco, but many "f" sounds in phage research are replaced with "ph".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 29, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage712
Genome length (bp)155060
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
MorphotypeMyoviridae
Number of Genes232
Number of tRNAs33
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Phusco_1

Phusco_2

Phusco_3
(nucleotidyltransferase)

Phusco_4

Phusco_5

Phusco_6

Phusco_7

Phusco_8

Phusco_9

Phusco_10

Phusco_11

Phusco_12

Phusco_13

Phusco_14

Phusco_15

Phusco_16

Phusco_17
(histidine triad nucleotide binding protein)

Phusco_18

Phusco_19

Phusco_20

Phusco_21

Phusco_22

Phusco_23

Phusco_24

Phusco_25

Phusco_26

Phusco_27

Phusco_28

Phusco_29

Phusco_30

Phusco_31

Phusco_32

Phusco_33

Phusco_34

Phusco_35
(ribbon-helix-helix DNA binding protein)

Phusco_36

Phusco_37

Phusco_38

Phusco_39

Phusco_40

Phusco_41

Phusco_42

Phusco_43

Phusco_44

Phusco_45

Phusco_46

Phusco_47

Phusco_48

Phusco_49

Phusco_50

Phusco_51

Phusco_52

Phusco_53

Phusco_54

Phusco_55

Phusco_56

Phusco_57

Phusco_58

Phusco_59

Phusco_60

Phusco_61

Phusco_62
(helix-turn-helix DNA binding domain protein)

Phusco_63
(helix-turn-helix DNA binding protein)

Phusco_64

Phusco_65

Phusco_66

Phusco_67

Phusco_68

Phusco_69

Phusco_70

Phusco_71

Phusco_72

Phusco_73

Phusco_74

Phusco_75

Phusco_76

Phusco_77

Phusco_78

Phusco_79

Phusco_80

Phusco_81

Phusco_82

Phusco_83

Phusco_84 — tRNA
(tRNA-Ser(gct))

Phusco_85 — tRNA
(tRNA-Leu(cag))

Phusco_86 — tRNA
(tRNA-Leu(gag))

Phusco_87 — tRNA
(tRNA-Leu(caa))

Phusco_88

Phusco_89

Phusco_90
(portal protein)

Phusco_91

Phusco_92

Phusco_93
(ThyX-like thymidylate synthase)

Phusco_94

Phusco_95
(RNA ligase)

Phusco_96

Phusco_97

Phusco_98

Phusco_99

Phusco_100
(major capsid protein)

Phusco_101

Phusco_102

Phusco_103

Phusco_104

Phusco_105

Phusco_106

Phusco_107

Phusco_108

Phusco_109

Phusco_110

Phusco_111

Phusco_112

Phusco_113

Phusco_114

Phusco_115

Phusco_116

Phusco_117

Phusco_118

Phusco_119

Phusco_120

Phusco_121
(acetyltransferase)

Phusco_122

Phusco_123

Phusco_124

Phusco_125

Phusco_126

Phusco_127
(tail sheath protein)

Phusco_128

Phusco_129

Phusco_130
(tail assembly chaperone)

Phusco_131
(tail assembly chaperone)

Phusco_132
(tape measure protein)

Phusco_133
(minor tail protein)

Phusco_134

Phusco_135

Phusco_136
(minor tail protein)

Phusco_137

Phusco_138

Phusco_139
(baseplate wedge protein)

Phusco_140
(baseplate wedge protein)

Phusco_141

Phusco_142

Phusco_143

Phusco_144
(minor tail protein)

Phusco_145
(minor tail protein)

Phusco_146

Phusco_147

Phusco_148

Phusco_149

Phusco_150

Phusco_151

Phusco_152

Phusco_153 — tRNA
(tRNA-Pro(tgg))

Phusco_154 — tRNA
(tRNA-Trp(cca))

Phusco_155 — tRNA
(tRNA-Tyr(gta))

Phusco_156

Phusco_157
(peptidyl tRNA hydrolase)

Phusco_158 — tRNA

Phusco_159 — tRNA
(tRNA-Met(cat))

Phusco_160 — tRNA
(tRNA-Cys(gca))

Phusco_161 — tRNA
(tRNA-Glu(ctc))

Phusco_162 — tRNA
(tRNA-His(gtg))

Phusco_163 — tRNA
(tRNA-Ala(tgc))

Phusco_164 — tRNA
(tRNA-Phe(gaa))

Phusco_165 — tRNA
(tRNA-Val(cac))

Phusco_166 — tRNA
(tRNA-Lys(ctt))

Phusco_167 — tRNA
(tRNA-Glu(ttc))

Phusco_168 — tRNA
(tRNA-Gly(tcc))

Phusco_169 — tRNA
(tRNA-Thr(cgt))

Phusco_170 — tRNA
(tRNA-Thr(tgt))

Phusco_171
(HNH endonuclease)

Phusco_172 — tRNA
(tRNA-Thr(ggt))

Phusco_173
(minor tail protein)

Phusco_174

Phusco_175 — tRNA
(tRNA-Gly(gcc))

Phusco_176 — tRNA
(tRNA-Asp(gtc))

Phusco_177 — tRNA
(tRNA-Met(cat))

Phusco_178 — tRNA
(tRNA-Ile(gat))

Phusco_179 — tRNA
(tRNA-Arg(acg))

Phusco_180 — tRNA
(tRNA-Val(gac))

Phusco_181 — tRNA
(tRNA-Arg(cct))

Phusco_182 — tmRNA
(tmRNA)

Phusco_183 — tRNA
(tRNA-Gln(ttg))

Phusco_184 — tRNA
(tRNA-Arg(tct))

Phusco_185 — tRNA
(tRNA-Lys(ttt))

Phusco_186

Phusco_187
(DNA helicase)

Phusco_188

Phusco_189

Phusco_190

Phusco_191

Phusco_192

Phusco_193

Phusco_194

Phusco_195

Phusco_196
(thymidylate kinase)

Phusco_197
(aminotransferase)

Phusco_198

Phusco_199

Phusco_200
(DnaC-like helicase loader)

Phusco_201
(DnaB-like dsDNA helicase)

Phusco_202

Phusco_203
(DNA primase)

Phusco_204

Phusco_205
(DnaJ-like chaperonin)

Phusco_206
(ssDNA binding protein)

Phusco_207
(DnaE-like DNA polymerase III)

Phusco_208
(RF-1 peptide chain release factor)

Phusco_209
(RecA-like DNA recombinase)

Phusco_210
(holliday junction resolvase)

Phusco_211

Phusco_212
(HNH endonuclease)

Phusco_213
(RusA-like resolvase)

Phusco_214

Phusco_215

Phusco_216

Phusco_217

Phusco_218

Phusco_219

Phusco_220
(HNH endonuclease)

Phusco_221

Phusco_222 — tRNA
(tRNA-Gln(ctg))

Phusco_223 — tRNA
(tRNA-Asn(gtt))

Phusco_224

Phusco_225

Phusco_226

Phusco_227

Phusco_228

Phusco_229

Phusco_230

Phusco_231

Phusco_232
(Ro-like RNA binding protein)

Phusco_233

Phusco_234

Phusco_235

Phusco_236

Phusco_237

Phusco_238

Phusco_239
(glycosyltransferase)

Phusco_240

Phusco_241
(glycosyltransferase)

Phusco_242
(glycosyltransferase)

Phusco_243
(PnuC-like nicotinamide riboside transporter)

Phusco_244
(glycosyltransferase)

Phusco_245

Phusco_246

Phusco_247

Phusco_248

Phusco_249
(lysin A)

Phusco_250

Phusco_251
(lysin B)

Phusco_252
(terminase)

Phusco_253

Phusco_254

Phusco_255

Phusco_256

Phusco_257
(polynucleotide kinase)

Phusco_258

Phusco_259

Phusco_260
(serine/threonine kinase)

Phusco_261

Phusco_262

Phusco_263
(PurA-like adenylosuccinate synthetase)

Phusco_264

Phusco_265

Phusco_266

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK359321
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 9
Pitt Freezer Box Grid# E4
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload