| Detailed Information for Phage Pier |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alia Elkhalili and Katie Zirbes |
| Year Found | 2012 |
| Location Found | Corpus Christi, TX USA |
| Finding Institution | Del Mar College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 27.764296, -97.407418 Map |
| Discovery Notes | - The temperature was about 97 degrees fahrenheit
- The air outside was very humid.
- We went down approximately 1 centimeter to retrieve the sample.
- The sample mostly consisted of sand and sea water. |
| Naming Notes | - The sample was named Pier because our soil was collected off of a fishing pier in Corpus Christi. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 22, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent |
| Approximate Shotgun Coverage | 360 |
| Sanger Finishing Reactions | 14 |
| Genome length (bp) | 156471 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were circular and approximately 1 mm in diameter and the area was 3.14 mm. The plaques were lytic because they are very well seen. |
| Morphotype | Myoviridae |
| Number of Genes | 234 |
| Number of tRNAs | 32 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG812494 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
35 |
| Pitt Freezer Box Grid# |
17 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |