| Detailed Information for Phage Pupusa |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Doris Arcos and Aeron Pearson |
| Year Found | 2025 |
| Location Found | Las Vegas, NV USA |
| Finding Institution | University of Nevada Las Vegas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 36.00639 N, 115.25111 E Map |
| Discovery Notes | The soil sample was taken from a home flower pot and appeared dry, dark brown, and loam-like in texture. It had an earthy, rich smell and was collected from a depth of 7 cm. The temperature at the time of sampling was 37.2°C with a humidity of 16%. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 489 |
| Genome length (bp) | 157602 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Nevada Las Vegas |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Morphotype | Myoviridae |
| Number of Genes | 236 |
| Number of tRNAs | 33 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
9.6 x 10^11 PFU/mL |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
