Mycobacterium phage Roots515
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Skelbel (AZ1) Keough (EK2) MamaT (GI) Lilo27 (EB) Liberone (GI)Mycobacterium phage Roots515
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| Detailed Information for Phage Roots515 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Courtney Mithelman |
| Year Found | 2017 |
| Location Found | Johnston, IA USA |
| Finding Institution | Northwestern College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.6597 N, 93.7207 W Map |
| Discovery Notes | Soil sample was wet and taken from slightly below surface level from a garden. Current outside temperature at time of selecting sample was 48 degrees Fahrenheit and raining. |
| Naming Notes | Sample was found in the Des Moines area, which has an area code of 515. Roots was used because Des Moines is my hometown. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 7, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 931 |
| Genome length (bp) | 156288 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Northwestern College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque was clear throughout purification steps. Size of plaque did change quite a bit at different purification points, varying from very large to very small. However, steps were taken to ensure that only one virus was present. |
| Morphotype | Myoviridae |
| Number of Genes | 237 |
| Number of tRNAs | 32 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH744422 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 72 |
| Pitt Freezer Box Grid# | H7 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |