| Detailed Information for Phage Shaqnato |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Myoseinge and Sinoseinge |
| Found By | Shaquille Fyfe |
| Year Found | 2017 |
| Location Found | Riverside, CA USA |
| Finding Institution | La Sierra University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.910183 N, 117.500914 W Map |
| Discovery Notes | On January 9, 2017 6:35pm, the researcher stumbled across wet loamy soil between the Administration building and Calkins, La Sierra University campus. The temperature was 12.7 degrees Celsius. The sample was collected 1-2 inches below the surface. |
| Naming Notes | The name 'myoseinge' derive from two words: myo and seinn. The scientific prefix myo meaning muscles symbolizes 'strong and hard-working'. Seinn is an old Norse word meaning slow or late as it is used to describe the character of the phage |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 10, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 650 |
| Genome length (bp) | 155596 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Small plaques with very clear margins. |
| Morphotype | Myoviridae |
| Number of Genes | 224 |
| Number of tRNAs | 33 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK359308 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.33x10^10 |
| Date of SEA Lysate Titering |
Feb 10, 2017 |
| Pitt Freezer Box# |
45 |
| Pitt Freezer Box Grid# |
C8 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
