| Detailed Information for Phage Shelob |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | DollyPharton |
| Found By | Evan Barnett and Maggie Wolf |
| Year Found | 2017 |
| Location Found | Evansville, IN USA |
| Finding Institution | University of Evansville |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 16°C |
| GPS Coordinates | 37.97139 N, 87.53027 W Map |
| Discovery Notes | Collected from the native plants garden on campus |
| Naming Notes | Literally all of our names were taken but let it be known that our child was originally going to be named Thicccccccq and she was beautiful |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 14, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 243 |
| Genome length (bp) | 156606 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Evansville |
| Annotation Status | In GenBank |
| Plaque Notes | Approximately 3/4mm in diameter, rough edges and slightly cloudy |
| Morphotype | Myoviridae |
| Number of Genes | 235 |
| Number of tRNAs | 32 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK450425 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.7 X 10^9 |
| Pitt Freezer Box# |
50 |
| Pitt Freezer Box Grid# |
C10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |