| Detailed Information for Phage Sprinklers |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kimberly Abels and Shannon Flynn |
| Year Found | 2014 |
| Location Found | Boulder, CO USA |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.0076 N, 105.2659 W Map |
| Discovery Notes | Moist, well-lit area, about an hour after the sprinkler ran
8/27/14
7:42 am, 16ºC |
| Naming Notes | Soil sample collected was near a sprinkler |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 9, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 885 |
| Genome length (bp) | 156060 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Colorado Boulder |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques
1-2 mm
Incubated 48 hours at 37ºC |
| Morphotype | Myoviridae |
| Number of Genes | 229 |
| Number of tRNAs | 35 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK359355 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.9x10e10 |
| Date of SEA Lysate Titering |
Sep 26, 2014 |
| Pitt Freezer Box# |
9 |
| Pitt Freezer Box Grid# |
B12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
