Mycobacterium phage Stubby
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Detailed Information for Phage Stubby
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMorgan Popek & Jennifer Britton
Year Found2015
Location FoundKenosha, WI USA
Finding InstitutionCarthage College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates42.63 N, 87.86 W Map
Naming NotesName was derived from the short tail of the phage, since it is a myoviridae. We found it to be stubby, so we named as such.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 19, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage376
Genome length (bp)153369
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesClear plaques, but often had mixed-size plaques. In the end, plaques were pinpricks.
MorphotypeMyoviridae
Number of Genes222
Number of tRNAs26
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Stubby_1

Stubby_2

Stubby_3
(HNH endonuclease)

Stubby_4

Stubby_5

Stubby_6

Stubby_7

Stubby_8

Stubby_9

Stubby_10

Stubby_11

Stubby_12

Stubby_13

Stubby_14

Stubby_15

Stubby_16

Stubby_17

Stubby_18

Stubby_19

Stubby_20

Stubby_21

Stubby_22

Stubby_23

Stubby_24

Stubby_25

Stubby_26

Stubby_27

Stubby_28

Stubby_29

Stubby_30

Stubby_31

Stubby_32

Stubby_33

Stubby_34

Stubby_35

Stubby_36

Stubby_37

Stubby_38

Stubby_39

Stubby_40

Stubby_41

Stubby_42

Stubby_43

Stubby_44

Stubby_45

Stubby_46

Stubby_47

Stubby_48

Stubby_49

Stubby_50

Stubby_51

Stubby_52

Stubby_53

Stubby_54

Stubby_55

Stubby_56

Stubby_57

Stubby_58
(helix-turn-helix DNA binding domain protein)

Stubby_59
(helix-turn-helix DNA binding protein)

Stubby_60

Stubby_61

Stubby_62

Stubby_63

Stubby_64

Stubby_65

Stubby_66

Stubby_67

Stubby_68

Stubby_69

Stubby_70

Stubby_71

Stubby_72

Stubby_73

Stubby_74

Stubby_75

Stubby_76

Stubby_77 — tRNA
(tRNA-Ser(gct))

Stubby_78 — tRNA
(tRNA-Leu(cag))

Stubby_79 — tRNA
(tRNA-Leu(gag))

Stubby_80 — tRNA
(tRNA-Leu(caa))

Stubby_81

Stubby_82

Stubby_83
(portal protein)

Stubby_84

Stubby_85

Stubby_86
(ThyX-like thymidylate synthase)

Stubby_87

Stubby_88
(RNA ligase)

Stubby_89

Stubby_90

Stubby_91

Stubby_92
(major capsid protein)

Stubby_93
(holliday junction resolvase)

Stubby_94

Stubby_95

Stubby_96

Stubby_97

Stubby_98

Stubby_99

Stubby_100

Stubby_101

Stubby_102

Stubby_103

Stubby_104

Stubby_105

Stubby_106

Stubby_107

Stubby_108

Stubby_109

Stubby_110

Stubby_111

Stubby_112

Stubby_113
(acetyltransferase)

Stubby_114

Stubby_115

Stubby_116

Stubby_117

Stubby_118

Stubby_119

Stubby_120
(tail sheath protein)

Stubby_121

Stubby_122

Stubby_123
(tail assembly chaperone)

Stubby_124
(tail assembly chaperone)

Stubby_125
(tape measure protein)

Stubby_126
(minor tail protein)

Stubby_127

Stubby_128

Stubby_129
(minor tail protein)

Stubby_130

Stubby_131

Stubby_132
(baseplate wedge protein)

Stubby_133
(baseplate wedge protein)

Stubby_134

Stubby_135

Stubby_136

Stubby_137
(minor tail protein)

Stubby_138
(minor tail protein)

Stubby_139

Stubby_140

Stubby_141

Stubby_142

Stubby_143

Stubby_144

Stubby_145 — tRNA
(tRNA-Pro(tgg))

Stubby_146 — tRNA
(tRNA-Trp(cca))

Stubby_147 — tRNA
(tRNA-Tyr(gta))

Stubby_148

Stubby_149

Stubby_150
(peptidyl tRNA hydrolase)

Stubby_151 — tRNA

Stubby_152 — tRNA
(tRNA-Met(cat))

Stubby_153 — tRNA
(tRNA-Cys(gca))

Stubby_154 — tRNA
(tRNA-Glu(ctc))

Stubby_155 — tRNA
(tRNA-His(gtg))

Stubby_156 — tRNA
(tRNA-Ala(tgc))

Stubby_157
(minor tail protein)

Stubby_158

Stubby_159 — tRNA
(tRNA-Gly(gcc))

Stubby_160 — tRNA
(tRNA-Asp(gtc))

Stubby_161 — tRNA
(tRNA-Met(cat))

Stubby_162 — tRNA
(tRNA-Ile(gat))

Stubby_163 — tRNA
(tRNA-Arg(acg))

Stubby_164 — tRNA
(tRNA-Val(gac))

Stubby_165 — tRNA
(tRNA-Arg(ccg))

Stubby_166 — tRNA
(tRNA-Arg(cct))

Stubby_167 — tmRNA
(tmRNA)

Stubby_168 — tRNA
(tRNA-Gln(ttg))

Stubby_169 — tRNA
(tRNA-Arg(tct))

Stubby_170 — tRNA
(tRNA-Lys(ttt))

Stubby_171

Stubby_172

Stubby_173
(DNA helicase)

Stubby_174

Stubby_175

Stubby_176

Stubby_177

Stubby_178

Stubby_179

Stubby_180

Stubby_181

Stubby_182
(thymidylate kinase)

Stubby_183
(aminotransferase)

Stubby_184

Stubby_185

Stubby_186
(DnaC-like helicase loader)

Stubby_187
(DnaB-like dsDNA helicase)

Stubby_188

Stubby_189
(DNA primase)

Stubby_190

Stubby_191
(DnaJ-like chaperonin)

Stubby_192
(ssDNA binding protein)

Stubby_193
(DnaE-like DNA polymerase III)

Stubby_194
(RF-1 peptide chain release factor)

Stubby_195
(RecA-like DNA recombinase)

Stubby_196
(holliday junction resolvase)

Stubby_197

Stubby_198
(HNH endonuclease)

Stubby_199
(RusA-like resolvase)

Stubby_200

Stubby_201

Stubby_202

Stubby_203

Stubby_204

Stubby_205

Stubby_206

Stubby_207 — tRNA
(tRNA-Gln(ctg))

Stubby_208 — tRNA
(tRNA-Asn(gtt))

Stubby_209

Stubby_210

Stubby_211

Stubby_212

Stubby_213

Stubby_214

Stubby_215

Stubby_216

Stubby_217
(Ro-like RNA binding protein)

Stubby_218

Stubby_219

Stubby_220

Stubby_221

Stubby_222

Stubby_223
(glycosyltransferase)

Stubby_224

Stubby_225
(glycosyltransferase)

Stubby_226
(glycosyltransferase)

Stubby_227

Stubby_228
(glycosyltransferase)

Stubby_229

Stubby_230

Stubby_231

Stubby_232

Stubby_233
(lysin A)

Stubby_234

Stubby_235
(lysin B)

Stubby_236
(terminase)

Stubby_237

Stubby_238

Stubby_239

Stubby_240

Stubby_241
(polynucleotide kinase)

Stubby_242

Stubby_243
(serine/threonine kinase)

Stubby_244

Stubby_245

Stubby_246
(PurA-like adenylosuccinate synthetase)

Stubby_247

Stubby_248

Stubby_249

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK450423
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 28
Pitt Freezer Box Grid# H3
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload