Mycobacterium phage StubbySaigey
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Detailed Information for Phage StubbySaigey
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySaige Stinson
Year Found2022
Location FoundWilliamsburg, VA United States of America
Finding InstitutionCollege of William & Mary
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.26556 N, 76.72286 W Map
Discovery NotesThe soil in which I found my phage came from Lake Matoaka. I got it along the edge of the river by the dock where student can rent canoes.
Naming NotesMy name is Saige and the phage I found has a short stubby tail.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage371
Genome length (bp)156308
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

Lethe (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionCollege of William & Mary
Annotation StatusIn GenBank
Plaque NotesThe phage I found had a small clear morphology.
MorphotypeMyoviridae
Number of Genes237
Number of tRNAs34
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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StubbySaigey_1

StubbySaigey_2

StubbySaigey_3
(nucleotidyl transferase)

StubbySaigey_4

StubbySaigey_5

StubbySaigey_6

StubbySaigey_7

StubbySaigey_8

StubbySaigey_9

StubbySaigey_10

StubbySaigey_11

StubbySaigey_12

StubbySaigey_13

StubbySaigey_14

StubbySaigey_15

StubbySaigey_16

StubbySaigey_17

StubbySaigey_18
(histidine triad nucleotide binding protein)

StubbySaigey_19

StubbySaigey_20

StubbySaigey_21

StubbySaigey_22

StubbySaigey_23

StubbySaigey_24

StubbySaigey_25

StubbySaigey_26

StubbySaigey_27

StubbySaigey_28
(band-7-like membrane protein)

StubbySaigey_29

StubbySaigey_30

StubbySaigey_31

StubbySaigey_32

StubbySaigey_33

StubbySaigey_34
(ribbon-helix-helix DNA binding domain protein)

StubbySaigey_35

StubbySaigey_36

StubbySaigey_37

StubbySaigey_38

StubbySaigey_39

StubbySaigey_40

StubbySaigey_41

StubbySaigey_42

StubbySaigey_43

StubbySaigey_44

StubbySaigey_45

StubbySaigey_46

StubbySaigey_47

StubbySaigey_48

StubbySaigey_49

StubbySaigey_50

StubbySaigey_51

StubbySaigey_52

StubbySaigey_53

StubbySaigey_54

StubbySaigey_55

StubbySaigey_56

StubbySaigey_57

StubbySaigey_58

StubbySaigey_59

StubbySaigey_60

StubbySaigey_61

StubbySaigey_62
(helix-turn-helix DNA binding domain protein)

StubbySaigey_63
(helix-turn-helix DNA binding domain protein)

StubbySaigey_64

StubbySaigey_65

StubbySaigey_66

StubbySaigey_67

StubbySaigey_68

StubbySaigey_69

StubbySaigey_70

StubbySaigey_71

StubbySaigey_72

StubbySaigey_73

StubbySaigey_74

StubbySaigey_75

StubbySaigey_76

StubbySaigey_77

StubbySaigey_78

StubbySaigey_79

StubbySaigey_80

StubbySaigey_81

StubbySaigey_82

StubbySaigey_83

StubbySaigey_84

StubbySaigey_85 — tRNA
(tRNA-Ser(gct))

StubbySaigey_86 — tRNA
(tRNA-Undet(nnn))

StubbySaigey_87 — tRNA
(tRNA-Leu(cag))

StubbySaigey_88 — tRNA
(tRNA-Leu(gag))

StubbySaigey_89 — tRNA
(tRNA-Leu(caa))

StubbySaigey_90

StubbySaigey_91

StubbySaigey_92
(portal protein)

StubbySaigey_93

StubbySaigey_94

StubbySaigey_95
(ThyX-like thymidylate synthase)

StubbySaigey_96

StubbySaigey_97
(RNA ligase)

StubbySaigey_98
(HNH endonuclease)

StubbySaigey_99
(LysM-like peptidoglycan binding protein)

StubbySaigey_100

StubbySaigey_101

StubbySaigey_102
(major capsid protein)

StubbySaigey_103
(Holliday junction resolvase)

StubbySaigey_104

StubbySaigey_105
(head fiber protein)

StubbySaigey_106

StubbySaigey_107

StubbySaigey_108

StubbySaigey_109

StubbySaigey_110

StubbySaigey_111

StubbySaigey_112

StubbySaigey_113

StubbySaigey_114

StubbySaigey_115

StubbySaigey_116

StubbySaigey_117

StubbySaigey_118

StubbySaigey_119

StubbySaigey_120

StubbySaigey_121

StubbySaigey_122

StubbySaigey_123

StubbySaigey_124
(acetyltransferase)

StubbySaigey_125

StubbySaigey_126

StubbySaigey_127

StubbySaigey_128

StubbySaigey_129

StubbySaigey_130

StubbySaigey_131
(tail sheath protein)

StubbySaigey_132

StubbySaigey_133

StubbySaigey_134
(tail assembly chaperone)

StubbySaigey_135
(tail assembly chaperone)

StubbySaigey_136
(tape measure protein)

StubbySaigey_137
(minor tail protein)

StubbySaigey_138

StubbySaigey_139

StubbySaigey_140
(minor tail protein)

StubbySaigey_141

StubbySaigey_142

StubbySaigey_143
(baseplate wedge protein)

StubbySaigey_144
(baseplate wedge protein)

StubbySaigey_145

StubbySaigey_146

StubbySaigey_147

StubbySaigey_148
(minor tail protein)

StubbySaigey_149
(minor tail protein)

StubbySaigey_150

StubbySaigey_151

StubbySaigey_152

StubbySaigey_153

StubbySaigey_154

StubbySaigey_155

StubbySaigey_156

StubbySaigey_157 — tRNA
(tRNA-Pro(tgg))

StubbySaigey_158 — tRNA
(tRNA-Trp(cca))

StubbySaigey_159 — tRNA
(tRNA-Tyr(gta))

StubbySaigey_160

StubbySaigey_161
(peptidyl tRNA hydrolase)

StubbySaigey_162 — tRNA
(tRNA-Pyl(cta))

StubbySaigey_163 — tRNA
(tRNA-Met(cat))

StubbySaigey_164 — tRNA
(tRNA-Cys(gca))

StubbySaigey_165 — tRNA
(tRNA-Glu(ctc))

StubbySaigey_166 — tRNA
(tRNA-His(gtg))

StubbySaigey_167 — tRNA
(tRNA-Ala(tgc))

StubbySaigey_168 — tRNA
(tRNA-Phe(gaa))

StubbySaigey_169 — tRNA
(tRNA-Val(cac))

StubbySaigey_170 — tRNA
(tRNA-Lys(ctt))

StubbySaigey_171 — tRNA
(tRNA-Glu(ttc))

StubbySaigey_172 — tRNA
(tRNA-Gly(tcc))

StubbySaigey_173 — tRNA
(tRNA-Thr(cgt))

StubbySaigey_174 — tRNA
(tRNA-Thr(tgt))

StubbySaigey_175
(HNH endonuclease)

StubbySaigey_176 — tRNA
(tRNA-Thr(ggt))

StubbySaigey_177
(minor tail protein)

StubbySaigey_178

StubbySaigey_179 — tRNA
(tRNA-Gly(gcc))

StubbySaigey_180 — tRNA
(tRNA-Asp(gtc))

StubbySaigey_181 — tRNA
(tRNA-Met(cat))

StubbySaigey_182 — tRNA
(tRNA-Ile(gat))

StubbySaigey_183 — tRNA
(tRNA-Arg(acg))

StubbySaigey_184 — tRNA
(tRNA-Val(gac))

StubbySaigey_185 — tRNA
(tRNA-Arg(cct))

StubbySaigey_186 — tmRNA
(Tag peptide: ATDTDATVTDAEIEAFFAEEAAALV*)

StubbySaigey_187 — tRNA
(tRNA-Gln(ttg))

StubbySaigey_188 — tRNA
(tRNA-Arg(tct))

StubbySaigey_189 — tRNA
(tRNA-Lys(ttt))

StubbySaigey_190

StubbySaigey_191

StubbySaigey_192
(DNA helicase)

StubbySaigey_193

StubbySaigey_194

StubbySaigey_195

StubbySaigey_196

StubbySaigey_197

StubbySaigey_198

StubbySaigey_199

StubbySaigey_200

StubbySaigey_201

StubbySaigey_202
(thymidylate kinase)

StubbySaigey_203
(aminotransferase)

StubbySaigey_204

StubbySaigey_205

StubbySaigey_206

StubbySaigey_207
(DnaC-like helicase loader)

StubbySaigey_208
(DnaB-like dsDNA helicase)

StubbySaigey_209

StubbySaigey_210
(DNA primase)

StubbySaigey_211

StubbySaigey_212
(DnaJ-like chaperonin)

StubbySaigey_213
(SSB protein)

StubbySaigey_214
(DnaE-like DNA polymerase III alpha)

StubbySaigey_215
(RF-1 peptide chain release factor)

StubbySaigey_216
(RecA-like DNA recombinase)

StubbySaigey_217
(Holliday junction resolvase)

StubbySaigey_218

StubbySaigey_219
(HNH endonuclease)

StubbySaigey_220
(RusA-like resolvase)

StubbySaigey_221

StubbySaigey_222

StubbySaigey_223

StubbySaigey_224

StubbySaigey_225
(glycoside hydrolase)

StubbySaigey_226

StubbySaigey_227

StubbySaigey_228 — tRNA
(tRNA-Gln(ctg))

StubbySaigey_229 — tRNA
(tRNA-Asn(gtt))

StubbySaigey_230

StubbySaigey_231

StubbySaigey_232

StubbySaigey_233

StubbySaigey_234

StubbySaigey_235

StubbySaigey_236

StubbySaigey_237

StubbySaigey_238
(Ro-like RNA binding protein)

StubbySaigey_239

StubbySaigey_240

StubbySaigey_241

StubbySaigey_242

StubbySaigey_243

StubbySaigey_244

StubbySaigey_245

StubbySaigey_246
(glycosyltransferase)

StubbySaigey_247

StubbySaigey_248
(glycosyltransferase)

StubbySaigey_249
(glycosyltransferase)

StubbySaigey_250
(PnuC-like nicotinamide riboside transporter)

StubbySaigey_251
(glycosyltransferase)

StubbySaigey_252

StubbySaigey_253

StubbySaigey_254

StubbySaigey_255

StubbySaigey_256
(HNH endonuclease)

StubbySaigey_257
(lysin A)

StubbySaigey_258
(holin)

StubbySaigey_259
(lysin B)

StubbySaigey_260
(terminase)

StubbySaigey_261

StubbySaigey_262

StubbySaigey_263

StubbySaigey_264

StubbySaigey_265
(polynucleotide kinase)

StubbySaigey_266

StubbySaigey_267
(serine/threonine kinase)

StubbySaigey_268

StubbySaigey_269
(PurA-like adenylosuccinate synthetase)

StubbySaigey_270

StubbySaigey_271

StubbySaigey_272

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR613482
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 160
Pitt Freezer Box Grid# F10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload