Mycobacterium phage Yassified
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Detailed Information for Phage Yassified
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByOwen Koetters
Year Found2022
Location FoundLos Angeles, CA United States
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.006988 N, 118.46831 W Map
Discovery NotesSample was moist and in an area replete with organic waste due to domesticated dogs and rats that live in the area.
Soil environment is well-shaded, densely packed with vegetables, and is thoroughly irrigated. Weather was sunny at the time of sampling.


Plated on MHA plate with 20 mM CaCl2
Naming NotesBy characterizing Yassified, we actively yassified it, which means to beautify or make something substantially better, which is done via isolation and through the process of visualizing the phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 26, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage167
Genome length (bp)155851
Character of genome endsCircularly Permuted
GC Content64.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

Lethe (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionSUNY Old Westbury
Annotation StatusIn GenBank
Plaque NotesClear plaques with a diameter that ranged from 1-2mm (1.5 mm avg)
MorphotypeMyoviridae
Number of Genes230
Number of tRNAs33
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Yassified_1

Yassified_2

Yassified_3
(nucleotidyl transferase)

Yassified_4

Yassified_5

Yassified_6
(HNH endonuclease)

Yassified_7

Yassified_8

Yassified_9

Yassified_10

Yassified_11

Yassified_12

Yassified_13

Yassified_14

Yassified_15

Yassified_16

Yassified_17

Yassified_18

Yassified_19

Yassified_20

Yassified_21

Yassified_22

Yassified_23

Yassified_24

Yassified_25

Yassified_26
(membrane protein, Band-7 -like)

Yassified_27

Yassified_28

Yassified_29

Yassified_30

Yassified_31

Yassified_32
(ribbon-helix-helix DNA binding domain)

Yassified_33

Yassified_34

Yassified_35

Yassified_36

Yassified_37

Yassified_38

Yassified_39

Yassified_40

Yassified_41

Yassified_42

Yassified_43

Yassified_44

Yassified_45
(helix-turn-helix DNA binding domain)

Yassified_46

Yassified_47

Yassified_48

Yassified_49

Yassified_50

Yassified_51

Yassified_52

Yassified_53

Yassified_54

Yassified_55

Yassified_56

Yassified_57

Yassified_58

Yassified_59

Yassified_60
(helix-turn-helix DNA binding domain)

Yassified_61
(helix-turn-helix DNA binding domain)

Yassified_62

Yassified_63

Yassified_64

Yassified_65

Yassified_66

Yassified_67

Yassified_68

Yassified_69

Yassified_70

Yassified_71

Yassified_72

Yassified_73

Yassified_74

Yassified_75

Yassified_76

Yassified_77

Yassified_78

Yassified_79

Yassified_80 — tRNA
(tRNA-Ser(gct))

Yassified_81 — tRNA
(tRNA-Leu(cag))

Yassified_82 — tRNA
(tRNA-Leu(gag))

Yassified_83 — tRNA
(tRNA-Leu(caa))

Yassified_84

Yassified_85

Yassified_86
(portal protein)

Yassified_87

Yassified_88

Yassified_89
(ThyX-like thymidylate synthase)

Yassified_90

Yassified_91
(RNA ligase)

Yassified_92
(HNH endonuclease)

Yassified_93

Yassified_94

Yassified_95

Yassified_96
(major capsid protein)

Yassified_97
(Holliday junction resolvase)

Yassified_98

Yassified_99
(head fiber protein)

Yassified_100

Yassified_101

Yassified_102

Yassified_103

Yassified_104

Yassified_105

Yassified_106

Yassified_107

Yassified_108

Yassified_109

Yassified_110

Yassified_111

Yassified_112

Yassified_113

Yassified_114

Yassified_115

Yassified_116

Yassified_117

Yassified_118
(acetyltransferase)

Yassified_119

Yassified_120

Yassified_121

Yassified_122

Yassified_123

Yassified_124
(tail sheath protein)

Yassified_125

Yassified_126

Yassified_127
(tail assembly chaperone)

Yassified_128
(tail assembly chaperone)

Yassified_129
(tape measure protein)

Yassified_130
(minor tail protein)

Yassified_131

Yassified_132

Yassified_133
(minor tail protein)

Yassified_134
(minor tail protein)

Yassified_135
(minor tail protein)

Yassified_136
(baseplate wedge protein)

Yassified_137
(baseplate J protein)

Yassified_138

Yassified_139

Yassified_140

Yassified_141
(minor tail protein)

Yassified_142
(minor tail protein)

Yassified_143

Yassified_144

Yassified_145

Yassified_146

Yassified_147

Yassified_148

Yassified_149

Yassified_150 — tRNA
(tRNA-Pro(tgg))

Yassified_151 — tRNA
(tRNA-Trp(cca))

Yassified_152 — tRNA
(tRNA-Tyr(gta))

Yassified_153

Yassified_154
(peptidyl tRNA hydrolase)

Yassified_155 — tRNA
(tRNA-Pyl(cta))

Yassified_156 — tRNA
(tRNA-Met(cat))

Yassified_157 — tRNA
(tRNA-Cys(gca))

Yassified_158 — tRNA
(tRNA-Glu(ctc))

Yassified_159 — tRNA
(tRNA-His(gtg))

Yassified_160 — tRNA
(tRNA-Ala(tgc))

Yassified_161 — tRNA
(tRNA-Phe(gaa))

Yassified_162 — tRNA
(tRNA-Val(cac))

Yassified_163 — tRNA
(tRNA-Lys(ctt))

Yassified_164 — tRNA
(tRNA-Glu(ttc))

Yassified_165 — tRNA
(tRNA-Gly(tcc))

Yassified_166 — tRNA
(tRNA-Thr(cgt))

Yassified_167 — tRNA
(tRNA-Thr(tgt))

Yassified_168
(HNH endonuclease)

Yassified_169 — tRNA
(tRNA-Thr(ggt))

Yassified_170
(minor tail protein)

Yassified_171

Yassified_172 — tRNA
(tRNA-Gly(gcc))

Yassified_173 — tRNA
(tRNA-Asp(gtc))

Yassified_174 — tRNA
(tRNA-Met(cat))

Yassified_175 — tRNA
(tRNA-Ile(gat))

Yassified_176 — tRNA
(tRNA-Arg(acg))

Yassified_177 — tRNA
(tRNA-Val(gac))

Yassified_178 — tRNA
(tRNA-Arg(cct))

Yassified_179 — tmRNA
(Tag peptide: ATDTDATVTDAEIEAFFAEEAAALV*)

Yassified_180 — tRNA
(tRNA-Gln(ttg))

Yassified_181 — tRNA
(tRNA-Arg(tct))

Yassified_182 — tRNA
(tRNA-Lys(ttt))

Yassified_183

Yassified_184
(DNA helicase)

Yassified_185

Yassified_186

Yassified_187

Yassified_188

Yassified_189

Yassified_190

Yassified_191

Yassified_192

Yassified_193

Yassified_194
(thymidylate kinase)

Yassified_195
(aminotransferase)

Yassified_196

Yassified_197

Yassified_198

Yassified_199
(DnaC-like helicase loader)

Yassified_200
(DnaB-like dsDNA helicase)

Yassified_201

Yassified_202
(DNA primase)

Yassified_203

Yassified_204
(chaperonin, DnaJ-like)

Yassified_205
(SSB protein)

Yassified_206
(DnaE-like DNA polymerase III (alpha))

Yassified_207
(RF-1 peptide chain release factor)

Yassified_208
(RecA-like DNA recombinase)

Yassified_209
(Holliday junction resolvase)

Yassified_210

Yassified_211
(HNH endonuclease)

Yassified_212
(RusA-like resolvase (endonuclease))

Yassified_213

Yassified_214
(DNA binding protein)

Yassified_215

Yassified_216

Yassified_217
(hydrolase)

Yassified_218

Yassified_219

Yassified_220 — tRNA
(tRNA-Gln(ctg))

Yassified_221 — tRNA
(tRNA-Asn(gtt))

Yassified_222

Yassified_223

Yassified_224

Yassified_225

Yassified_226

Yassified_227

Yassified_228

Yassified_229

Yassified_230
(Ro-like RNA binding protein)

Yassified_231

Yassified_232

Yassified_233

Yassified_234

Yassified_235

Yassified_236

Yassified_237
(glycosyltransferase)

Yassified_238
(glycosyltransferase)

Yassified_239
(glycosyltransferase)

Yassified_240
(PnuC-like Nicotinamide riboside transporter)

Yassified_241
(glycosyltransferase)

Yassified_242

Yassified_243

Yassified_244

Yassified_245

Yassified_246
(lysin A)

Yassified_247
(holin)

Yassified_248
(lysin B)

Yassified_249
(terminase)

Yassified_250

Yassified_251

Yassified_252

Yassified_253

Yassified_254
(polynucleotide kinase)

Yassified_255

Yassified_256

Yassified_257
(serine/threonine kinase)

Yassified_258
(HNH endonuclease)

Yassified_259

Yassified_260
(adenylosuccinate synthetase PurA-like)

Yassified_261

Yassified_262

Yassified_263

Yassified_264

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR613471
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 156
Pitt Freezer Box Grid# A3
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload