| Detailed Information for Phage Yucca |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | R. Lane Shafer |
| Year Found | 2015 |
| Location Found | Bloomington, IL USA |
| Finding Institution | Illinois Wesleyan University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 19°C |
| GPS Coordinates | 40.4825 N, 88.979722 W Map |
| Discovery Notes | Soil sample taken from Sarah Davis garden (on the David Davis mansion), which dates back to 1871. The garden is located in a fairly urban area, in close proximity to footpaths and sample is located close to other plants. The soil was taken from about 9 cm deep and the soil itself is dark and moist.
*Phage isolated using Direct Plating technique |
| Naming Notes | The name originates from the genus of the plant that the sample was taken from. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 110 |
| Genome length (bp) | 155582 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Typically small plaques, varying in size from .5 - 2.0 mm, and averages 1.5 mm in diameter. Plaques are clear and concise with no bullseye appearance. Plaques take about 48 hours to fully develop. |
| Morphotype | Myoviridae |
| Number of Genes | 232 |
| Number of tRNAs | 35 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX721255 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.65 x 10^10 |
| Date of SEA Lysate Titering |
Sep 27, 2015 |
| Pitt Freezer Box# |
24 |
| Pitt Freezer Box Grid# |
H3 |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
