Mycobacterium phage Chomp
Chomp Plaque Picture
Chomp EM Picture
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Detailed Information for Phage Chomp
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByQuinn Klessel
Year Found2021
Location FoundNorth Wales, PA United States
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature7°C
GPS Coordinates40.21221 N, 75.23547 W Map
Discovery NotesChomp was collected from 1 cm below the surface on a day with an ambient temperature of 7 degrees Celsius. The soil sample was collected from under a bridge that runs over a small creek in Oxford Park in North Wales Pennsylvania. The soil sample was damp and was full of organic matter.
Naming NotesThis phage was named Chomp as a humorous note on how bacteriophages eat bacteria. This phage "chomps" bacteria.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 17, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage135
Genome length (bp)156655
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionLehigh University
Annotation StatusIn GenBank
Plaque NotesChomp produced plaques with clear centers and cloudy edges. The morphology of the plaques was relatively uniform with a diameter averaging about 1 millimeter. Chomp on occasion produced cloudy plaques, it is being researched further.
MorphotypeMyoviridae
Number of Genes231
Number of tRNAs32
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Chomp_1

Chomp_2

Chomp_3
(nucleotidyl transferase)

Chomp_4

Chomp_5

Chomp_6

Chomp_7

Chomp_8

Chomp_9

Chomp_10

Chomp_11

Chomp_12

Chomp_13

Chomp_14

Chomp_15

Chomp_16

Chomp_17
(histidine triad nucleotide binding protein)

Chomp_18

Chomp_19

Chomp_20

Chomp_21

Chomp_22

Chomp_23

Chomp_24

Chomp_25
(HNH endonuclease)

Chomp_26

Chomp_27

Chomp_28

Chomp_29
(Band-7 like membrane protein)

Chomp_30

Chomp_31

Chomp_32

Chomp_33

Chomp_34

Chomp_35
(ribbon-helix-helix DNA binding domain)

Chomp_36

Chomp_37

Chomp_38

Chomp_39

Chomp_40

Chomp_41

Chomp_42

Chomp_43

Chomp_44

Chomp_45

Chomp_46

Chomp_47

Chomp_48

Chomp_49

Chomp_50

Chomp_51

Chomp_52

Chomp_53

Chomp_54

Chomp_55

Chomp_56

Chomp_57

Chomp_58

Chomp_59

Chomp_60

Chomp_61
(helix-turn-helix DNA binding domain)

Chomp_62
(helix-turn-helix DNA binding domain)

Chomp_63

Chomp_64

Chomp_65

Chomp_66

Chomp_67

Chomp_68

Chomp_69

Chomp_70

Chomp_71

Chomp_72

Chomp_73

Chomp_74

Chomp_75

Chomp_76

Chomp_77

Chomp_78

Chomp_79

Chomp_80

Chomp_81

Chomp_82

Chomp_83 — tRNA
(tRNA-Ser(gct))

Chomp_84 — tRNA
(tRNA-Leu(cag))

Chomp_85 — tRNA
(tRNA-Leu(gag))

Chomp_86 — tRNA
(tRNA-Leu(caa))

Chomp_87

Chomp_88

Chomp_89
(portal protein)

Chomp_90

Chomp_91

Chomp_92
(ThyX-like thymidylate synthase)

Chomp_93

Chomp_94
(RNA ligase)

Chomp_95

Chomp_96

Chomp_97

Chomp_98

Chomp_99
(major capsid protein)

Chomp_100

Chomp_101
(head fiber protein)

Chomp_102

Chomp_103

Chomp_104

Chomp_105

Chomp_106

Chomp_107

Chomp_108

Chomp_109

Chomp_110

Chomp_111

Chomp_112

Chomp_113

Chomp_114

Chomp_115

Chomp_116

Chomp_117

Chomp_118

Chomp_119

Chomp_120

Chomp_121

Chomp_122

Chomp_123

Chomp_124

Chomp_125

Chomp_126

Chomp_127
(tail sheath protein)

Chomp_128

Chomp_129

Chomp_130
(tail assembly chaperone)

Chomp_131
(tail assembly chaperone)

Chomp_132
(tape measure protein)

Chomp_133

Chomp_134

Chomp_135

Chomp_136
(minor tail protein)

Chomp_137
(minor tail protein)

Chomp_138
(minor tail protein)

Chomp_139
(baseplate wedge protein)

Chomp_140
(baseplate J protein)

Chomp_141

Chomp_142

Chomp_143

Chomp_144

Chomp_145
(minor tail protein)

Chomp_146

Chomp_147

Chomp_148

Chomp_149

Chomp_150

Chomp_151

Chomp_152

Chomp_153 — tRNA
(tRNA-Pro(tgg))

Chomp_154 — tRNA
(tRNA-Trp(cca))

Chomp_155 — tRNA
(tRNA-Tyr(gta))

Chomp_156

Chomp_157
(peptidyl tRNA hydrolase)

Chomp_158 — tRNA
(tRNA-pyl(cta))

Chomp_159 — tRNA
(fMet; tRNA-fMet(cat))

Chomp_160 — tRNA
(tRNA-Cys(gca))

Chomp_161 — tRNA
(tRNA-Glu(ctc))

Chomp_162 — tRNA
(tRNA-His(gtg))

Chomp_163 — tRNA
(tRNA-Ala(tgc))

Chomp_164

Chomp_165 — tRNA
(tRNA-Phe(gaa))

Chomp_166 — tRNA
(tRNA-Val(cac))

Chomp_167 — tRNA
(tRNA-Lys(ctt))

Chomp_168 — tRNA
(tRNA-Glu(ttc))

Chomp_169 — tRNA
(tRNA-Gly(tcc))

Chomp_170 — tRNA
(tRNA-Thr(cgt))

Chomp_171 — tRNA
(tRNA-Thr(tgt))

Chomp_172
(HNH endonuclease)

Chomp_173 — tRNA
(tRNA-Thr(ggt))

Chomp_174
(minor tail protein)

Chomp_175

Chomp_176 — tRNA
(tRNA-Gly(gcc))

Chomp_177 — tRNA
(tRNA-Asp(gtc))

Chomp_178 — tRNA
(tRNA-Ile2(cat))

Chomp_179 — tRNA
(tRNA-Ile(gat))

Chomp_180 — tRNA
(tRNA-Arg(acg))

Chomp_181 — tRNA
(tRNA-Val(gac))

Chomp_182 — tRNA
(tRNA-Arg(cct))

Chomp_183 — tmRNA
(Tag peptide: ATDTDATVTDAEIEAFFAEEAAALV*)

Chomp_184 — tRNA
(tRNA-Gln(ttg))

Chomp_185 — tRNA
(tRNA-Arg(tct))

Chomp_186

Chomp_187
(DNA helicase)

Chomp_188

Chomp_189

Chomp_190

Chomp_191

Chomp_192

Chomp_193

Chomp_194

Chomp_195

Chomp_196

Chomp_197
(thymidylate kinase)

Chomp_198
(aminotransferase)

Chomp_199

Chomp_200

Chomp_201

Chomp_202
(DnaC-like helicase loader)

Chomp_203
(DnaB-like dsDNA helicase)

Chomp_204

Chomp_205
(DNA primase)

Chomp_206

Chomp_207
(DnaJ-like chaperonin)

Chomp_208
(SSB protein)

Chomp_209
(DnaE-like DNA polymerase III)

Chomp_210
(RF-1 peptide chain release factor)

Chomp_211
(RecA-like DNA recombinase)

Chomp_212
(Holliday junction resolvase)

Chomp_213

Chomp_214
(HNH endonuclease)

Chomp_215
(RusA-like resolvase)

Chomp_216

Chomp_217

Chomp_218

Chomp_219

Chomp_220

Chomp_221

Chomp_222
(HNH endonuclease)

Chomp_223

Chomp_224 — tRNA
(tRNA-Gln(ctg))

Chomp_225 — tRNA
(tRNA-Asn(gtt))

Chomp_226

Chomp_227

Chomp_228

Chomp_229

Chomp_230

Chomp_231

Chomp_232
(Ro-like RNA binding protein)

Chomp_233

Chomp_234

Chomp_235

Chomp_236

Chomp_237

Chomp_238

Chomp_239
(glycosyltransferase)

Chomp_240

Chomp_241
(glycosyltransferase)

Chomp_242
(glycosyltransferase)

Chomp_243
(PnuC-like nicotinamide riboside transporter)

Chomp_244
(glycosyltransferase)

Chomp_245

Chomp_246

Chomp_247

Chomp_248

Chomp_249
(lysin A)

Chomp_250
(holin)

Chomp_251
(lysin B)

Chomp_252
(terminase)

Chomp_253

Chomp_254

Chomp_255

Chomp_256

Chomp_257
(polynucleotide kinase)

Chomp_258

Chomp_259
(serine/threonine kinase)

Chomp_260

Chomp_261

Chomp_262
(PurA-like adenylosuccinate synthetase)

Chomp_263

Chomp_264

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP978875
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 8.0 * 10^9 pfu/mL
Pitt Freezer Box# 142
Pitt Freezer Box Grid# H11
Available Files
GenBank File for PhameratorDownload