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Mycobacterium phage CindyLou

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Detailed Information for Phage CindyLou
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Alexa Mihaita
Year Found	2021
Location Found	Springfield, PA United States of America
Finding Institution	Saint Joseph's University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	-1°C
GPS Coordinates	39.950167 N, 75.334002 W Map
Discovery Notes	The soil sample containing phage CindyLou was collected on January 23rd at 10:51 AM from beneath an evergreen tree next to the deck at Alexa's home in Springfield, Pennsylvania. The soil was recovered from a depth of 45.72 cm and was moist. The ambient humidity was 34%, and the weather was cold, dry, and mostly cloudy with 21 mph gusts of wind. The HTL titer was 6.8 x 10^9 pfu/ml. The DNA concentration was 419.2 ng/ul. Based on the electron micrograph, CindyLou's average capsid diameter was 68.6 nm, and the average tail length was 92.9 nm (n=3). A PCR test using C1-specific primers strongly amplified CindyLou's DNA, providing evidence that CindyLou may belong to the C1 cluster.
Naming Notes	The phage was named CindyLou after the very tiny Dr. Seuss character, since the phage always yielded very small plaques from 0.5 mm - 1 mm in diameter.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Aug 9, 2021
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	68
Genome length (bp)	157677
Character of genome ends	Circularly Permuted
GC Content	64.6%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	C
Subcluster	C1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Ading (C1) Adlitam (C1) Alice (C1) Amataga (C1) ArcherS7 (C1) Asterius (C1) Astraea (C1) Atlantean (C1) Audrick (C1) Ava3 (C1) Babyland (C1) BackyardAgain (C1) BadAgartude (C1) BananaFence (C1) Bangla1971 (C1) Basquiat (C1) BeanWater (C1) BigCity (C1) Bigswole (C1) Bipolarisk (C1) Blackbrain (C1) Blackdragon (C1) Blubba (C1) Bonray (C1) Bread (C1) Breeniome (C1) Burrough (C1) Bxz1 (C1) Cactojaque (C1) Cali (C1) Cane17 (C1) Capablanca (C1) Caravan (C1) Catera (C1) Chargie21 (C1) CharlieB (C1) ChaylaJr (C1) ChickenPhender (C1) Chomp (C1) CindyLou (C1) Colt (C1) Concombre (C1) Daffodil (C1) Dandelion (C1) Darko (C1) Delilah (C1) Delylah (C1) Demodog (C1) Derek (C1) Dietrick (C1) DirtMonster (C1) Domi (C1) Drazdys (C1) DrPhinkDaddy (C1) DTDevon (C1) EasyJones (C1) EggyFarm (C1) EmmaElysia (C1) EmToTheThree (C1) Erdmann (C1) ErnieJ (C1) Essence (C1) ET08 (C1) Ewok (C1) Flabslab (C1) Fludd (C1) FoxtrotP1 (C1) FrayBell (C1) FudgeTart (C1) Gabriel (C1) Gamgam (C1) Ghost (C1) Gizmo (C1) Grasshills (C1) Grungle (C1) Guwapp (C1) Halldule (C1) HyRo (C1) I3 (C1) IkeLoa (C1) InigoMontoya (C1) InterFolia (C1) Iota (C1) Issimir (C1) Izajani (C1) Janiyra (C1) Jaygup (C1) JayJay (C1) Jedediah (C1) Jemmno (C1) Jessibeth14 (C1) JigglyPuff (C1) JPickles (C1) JulietS (C1) JustHall (C1) Kamryn (C1) Kboogie (C1) Khaleesi (C1) Koguma (C1) Latch (C1) Lee7 (C1) LifeSavor (C1) LinStu (C1) Littleton (C1) Loanshark (C1) LolaVinca (C1) LordLeafolot (C1) LRRHood (C1) Lukilu (C1) Maby2021 (C1) Mangeria (C1) Matsumoto (C1) McGee (C1) McWolfish (C1) Megamind (C1) Melpomini (C1) MikeLiesIn (C1) Mikro (C1) Momo (C1) MoMoMixon (C1) Morizzled23 (C1) Naija (C1) Napoleon13 (C1) Nappy (C1) Naval22 (C1) NedWong (C1) Ngannou (C1) Nidhogg (C1) NoodleTree (C1) Norm (C1) NuevoMundo (C1) ParkTD (C1) Patter (C1) Phlegm (C1) Phox (C1) Phusco (C1) Pier (C1) Pinkcreek (C1) Pio (C1) Pivoine (C1) Pleione (C1) PurduePete (C1) QBert (C1) Quasimodo (C1) Rabinovish (C1) Rahel (C1) Rizal (C1) RoMag (C1) Ronan (C1) Roots515 (C1) Salacia (C1) Sauce (C1) ScottMcG (C1) Sebata (C1) Shaqnato (C1) Shelob (C1) ShiaLabeouf (C1) Shifa (C1) Shnickers (C1) Shrimp (C1) SilverDipper (C1) SmallFry (C1) Spec (C1) Specks (C1) Sprinklers (C1) Spud (C1) SrishMeg2525 (C1) StephanieG (C1) Stubby (C1) StubbySaigey (C1) Tainan (C1) Teardrop (C1) TinyTim (C1) Tonenili (C1) Tortoise16 (C1) Trinitium (C1) Turret (C1) Tyke (C1) ValleyTerrace (C1) Wally (C1) Willis (C1) Yassified (C1) YemiJoy2021 (C1) YoungMoneyMata (C1) Yucca (C1) Zalkecks (C1) Zeenon (C1) ZygoTaiga (C1) Greely (C2) Myrna (C2) Phabba (C2) ScoobyDoobyDoo (C2)
Annotating Institution	Saint Joseph's University
Annotation Status	In GenBank
Plaque Notes	Plaques are circular and clear, with an average diameter of about 0.8 mm after a 24 hour incubation period.
Morphotype	Myoviridae
Number of Genes	234
Number of tRNAs	32
Number of tmRNAs	1
Has been Phamerated?	Yes
Gene List	Click to View CindyLou_1 CindyLou_2 CindyLou_3 (nucleotidyltransferase) CindyLou_4 CindyLou_5 CindyLou_6 CindyLou_7 CindyLou_8 CindyLou_9 CindyLou_10 CindyLou_11 CindyLou_12 CindyLou_13 CindyLou_14 CindyLou_15 CindyLou_16 CindyLou_17 (histidine triad nucleotide binding protein) CindyLou_18 CindyLou_19 CindyLou_20 CindyLou_21 CindyLou_22 CindyLou_23 CindyLou_24 CindyLou_25 (HNH endonuclease) CindyLou_26 CindyLou_27 CindyLou_28 CindyLou_29 (band-7-like membrane protein) CindyLou_30 CindyLou_31 CindyLou_32 CindyLou_33 CindyLou_34 CindyLou_35 (ribbon-helix-helix DNA binding domain protein) CindyLou_36 CindyLou_37 CindyLou_38 CindyLou_39 CindyLou_40 CindyLou_41 CindyLou_42 CindyLou_43 CindyLou_44 CindyLou_45 CindyLou_46 CindyLou_47 CindyLou_48 CindyLou_49 CindyLou_50 CindyLou_51 CindyLou_52 CindyLou_53 CindyLou_54 CindyLou_55 CindyLou_56 CindyLou_57 CindyLou_58 CindyLou_59 CindyLou_60 CindyLou_61 CindyLou_62 CindyLou_63 (helix-turn-helix DNA binding domain protein) CindyLou_64 (helix-turn-helix DNA binding domain protein) CindyLou_65 CindyLou_66 CindyLou_67 CindyLou_68 CindyLou_69 CindyLou_70 CindyLou_71 CindyLou_72 CindyLou_73 CindyLou_74 CindyLou_75 CindyLou_76 CindyLou_77 CindyLou_78 CindyLou_79 CindyLou_80 CindyLou_81 CindyLou_82 CindyLou_83 CindyLou_84 (ParB-like nuclease domain protein) CindyLou_85 — tRNA (tRNA-Ser(gct)) CindyLou_86 — tRNA (tRNA-Leu(cag)) CindyLou_87 — tRNA (tRNA-Leu(gag)) CindyLou_88 — tRNA (tRNA-Leu(caa)) CindyLou_89 CindyLou_90 CindyLou_91 (portal protein) CindyLou_92 CindyLou_93 CindyLou_94 (ThyX-like thymidylate synthase) CindyLou_95 CindyLou_96 (RNA ligase) CindyLou_97 (HNH endonuclease) CindyLou_98 CindyLou_99 CindyLou_100 CindyLou_101 CindyLou_102 CindyLou_103 CindyLou_104 CindyLou_105 CindyLou_106 CindyLou_107 CindyLou_108 CindyLou_109 CindyLou_110 CindyLou_111 CindyLou_112 CindyLou_113 CindyLou_114 CindyLou_115 CindyLou_116 CindyLou_117 CindyLou_118 CindyLou_119 CindyLou_120 CindyLou_121 CindyLou_122 CindyLou_123 CindyLou_124 CindyLou_125 CindyLou_126 CindyLou_127 CindyLou_128 CindyLou_129 (tail sheath protein) CindyLou_130 CindyLou_131 CindyLou_132 (tail assembly chaperone) CindyLou_133 (tail assembly chaperone) CindyLou_134 (tape measure protein) CindyLou_135 (minor tail protein) CindyLou_136 CindyLou_137 CindyLou_138 (minor tail protein) CindyLou_139 CindyLou_140 (minor tail protein) CindyLou_141 (baseplate wedge protein) CindyLou_142 (baseplate J protein) CindyLou_143 CindyLou_144 CindyLou_145 CindyLou_146 (minor tail protein) CindyLou_147 (minor tail protein) CindyLou_148 CindyLou_149 CindyLou_150 CindyLou_151 CindyLou_152 CindyLou_153 CindyLou_154 CindyLou_155 CindyLou_156 — tRNA (tRNA-Pro(tgg)) CindyLou_157 — tRNA (tRNA-Trp(cca)) CindyLou_158 — tRNA (tRNA-Tyr(gta)) CindyLou_159 CindyLou_160 (peptidyl tRNA hydrolase) CindyLou_161 — tRNA (tRNA-Pyl(cta)) CindyLou_162 — tRNA (tRNA-Met(cat)) CindyLou_163 — tRNA (tRNA-Cys(gca)) CindyLou_164 — tRNA (tRNA-Glu(ctc)) CindyLou_165 — tRNA (tRNA-His(gtg)) CindyLou_166 — tRNA (tRNA-Ala(tgc)) CindyLou_167 — tRNA (tRNA-Phe(gaa)) CindyLou_168 — tRNA (tRNA-Val(cac)) CindyLou_169 — tRNA (tRNA-Lys(ctt)) CindyLou_170 — tRNA (tRNA-Glu(ttc)) CindyLou_171 — tRNA (tRNA-Gly(tcc)) CindyLou_172 — tRNA (tRNA-Thr(cgt)) CindyLou_173 — tRNA (tRNA-Thr(tgt)) CindyLou_174 (HNH endonuclease) CindyLou_175 — tRNA (tRNA-Thr(ggt)) CindyLou_176 (minor tail protein) CindyLou_177 CindyLou_178 — tRNA (tRNA-Gly(gcc)) CindyLou_179 — tRNA (tRNA-Asp(gtc)) CindyLou_180 — tRNA (tRNA-Met(cat)) CindyLou_181 — tRNA (tRNA-Ile(gat)) CindyLou_182 — tRNA (tRNA-Arg(acg)) CindyLou_183 — tRNA (tRNA-Val(gac)) CindyLou_184 — tRNA (tRNA-Arg(cct)) CindyLou_185 — tmRNA (Tag peptide: ATDTDATVTDAEIEAFFAEEAAALV*) CindyLou_186 — tRNA (tRNA-Gln(ttg)) CindyLou_187 — tRNA (tRNA-Arg(tct)) CindyLou_188 CindyLou_189 (DNA helicase) CindyLou_190 CindyLou_191 CindyLou_192 CindyLou_193 CindyLou_194 CindyLou_195 CindyLou_196 CindyLou_197 CindyLou_198 (thymidylate kinase) CindyLou_199 (aminotransferase) CindyLou_200 CindyLou_201 CindyLou_202 CindyLou_203 (DnaC-like helicase loader) CindyLou_204 (DnaB-like dsDNA helicase) CindyLou_205 CindyLou_206 (DNA primase) CindyLou_207 CindyLou_208 (DnaJ-like chaperonin) CindyLou_209 (ssDNA binding protein) CindyLou_210 (DnaE-like DNA polymerase III alpha) CindyLou_211 (RF-1 peptide chain release factor) CindyLou_212 (RecA-like DNA recombinase) CindyLou_213 (holliday junction resolvase) CindyLou_214 CindyLou_215 (HNH endonuclease) CindyLou_216 (RusA-like resolvase) CindyLou_217 CindyLou_218 CindyLou_219 CindyLou_220 CindyLou_221 CindyLou_222 CindyLou_223 CindyLou_224 — tRNA (tRNA-Gln(ctg)) CindyLou_225 — tRNA (tRNA-Asn(gtt)) CindyLou_226 CindyLou_227 CindyLou_228 CindyLou_229 CindyLou_230 CindyLou_231 CindyLou_232 CindyLou_233 CindyLou_234 (Ro-like RNA binding protein) CindyLou_235 CindyLou_236 CindyLou_237 CindyLou_238 CindyLou_239 CindyLou_240 CindyLou_241 (glycosyltransferase) CindyLou_242 CindyLou_243 (glycosyltransferase) CindyLou_244 (glycosyltransferase) CindyLou_245 (PnuC-like nicotinamide riboside transporter) CindyLou_246 (glycosyltransferase) CindyLou_247 CindyLou_248 CindyLou_249 CindyLou_250 CindyLou_251 (lysin A) CindyLou_252 (holin) CindyLou_253 (lysin B) CindyLou_254 (terminase) CindyLou_255 CindyLou_256 CindyLou_257 CindyLou_258 CindyLou_259 (polynucleotide kinase) CindyLou_260 CindyLou_261 (serine/threonine kinase) CindyLou_262 CindyLou_263 CindyLou_264 (PurA-like adenylosuccinate synthetase) CindyLou_265 CindyLou_266 CindyLou_267
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	ON081335
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	129
Pitt Freezer Box Grid#	H6
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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