Mycobacterium phage Daffodil
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Detailed Information for Phage Daffodil
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBrooke Koebele
Year Found2015
Location FoundBloomington, IL USA
Finding InstitutionIllinois Wesleyan University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates40.491667 N, 88.991944 W Map
Discovery NotesFound on a sunny day
slightly windy
dug five inches below the surface
No grass or weeds around- fresh dirt
Naming NotesI choose daffodil because it means rebirth and happiness, and I hope my phage is used for good and help society.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 2, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1206
Genome length (bp)155034
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesMy plaques are very small and round. They usually take about 48 hours to fully appear.
MorphotypeMyoviridae
Number of Genes238
Number of tRNAs34
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Daffodil_1

Daffodil_2

Daffodil_3
(nucleotidyltransferase)

Daffodil_4

Daffodil_5

Daffodil_6

Daffodil_7

Daffodil_8

Daffodil_9

Daffodil_10

Daffodil_11

Daffodil_12

Daffodil_13

Daffodil_14

Daffodil_15

Daffodil_16

Daffodil_17

Daffodil_18

Daffodil_19

Daffodil_20
(histidine triad nucleotide binding protein)

Daffodil_21

Daffodil_22

Daffodil_23

Daffodil_24

Daffodil_25

Daffodil_26

Daffodil_27

Daffodil_28

Daffodil_29

Daffodil_30

Daffodil_31
(band-7-like membrane protein)

Daffodil_32

Daffodil_33

Daffodil_34

Daffodil_35

Daffodil_36

Daffodil_37

Daffodil_38

Daffodil_39

Daffodil_40

Daffodil_41

Daffodil_42

Daffodil_43

Daffodil_44

Daffodil_45

Daffodil_46

Daffodil_47

Daffodil_48

Daffodil_49

Daffodil_50

Daffodil_51

Daffodil_52

Daffodil_53

Daffodil_54

Daffodil_55

Daffodil_56

Daffodil_57

Daffodil_58

Daffodil_59

Daffodil_60

Daffodil_61

Daffodil_62

Daffodil_63

Daffodil_64

Daffodil_65
(helix-turn-helix DNA binding domain protein)

Daffodil_66
(helix-turn-helix DNA binding protein)

Daffodil_67

Daffodil_68

Daffodil_69

Daffodil_70

Daffodil_71

Daffodil_72

Daffodil_73

Daffodil_74

Daffodil_75

Daffodil_76

Daffodil_77

Daffodil_78

Daffodil_79

Daffodil_80

Daffodil_81

Daffodil_82

Daffodil_83

Daffodil_84

Daffodil_85

Daffodil_86

Daffodil_87

Daffodil_88 — tRNA
(tRNA-Ser(gct))

Daffodil_89 — tRNA
(tRNA-Ser(cga))

Daffodil_90 — tRNA
(tRNA-Leu(cag))

Daffodil_91 — tRNA
(tRNA-Leu(gag))

Daffodil_92 — tRNA
(tRNA-Leu(caa))

Daffodil_93

Daffodil_94

Daffodil_95
(portal protein)

Daffodil_96

Daffodil_97

Daffodil_98
(ThyX-like thymidylate synthase)

Daffodil_99

Daffodil_100
(RNA ligase)

Daffodil_101

Daffodil_102

Daffodil_103

Daffodil_104
(major capsid protein)

Daffodil_105

Daffodil_106

Daffodil_107

Daffodil_108

Daffodil_109

Daffodil_110

Daffodil_111

Daffodil_112

Daffodil_113

Daffodil_114

Daffodil_115

Daffodil_116

Daffodil_117

Daffodil_118

Daffodil_119

Daffodil_120

Daffodil_121

Daffodil_122

Daffodil_123

Daffodil_124

Daffodil_125

Daffodil_126

Daffodil_127

Daffodil_128

Daffodil_129

Daffodil_130

Daffodil_131

Daffodil_132
(tail sheath protein)

Daffodil_133

Daffodil_134

Daffodil_135
(tail assembly chaperone)

Daffodil_136
(tail assembly chaperone)

Daffodil_137
(tape measure protein)

Daffodil_138

Daffodil_139

Daffodil_140

Daffodil_141
(minor tail protein)

Daffodil_142

Daffodil_143
(minor tail protein)

Daffodil_144
(baseplate wedge protein)

Daffodil_145
(baseplate J protein)

Daffodil_146

Daffodil_147

Daffodil_148

Daffodil_149

Daffodil_150
(minor tail protein)

Daffodil_151

Daffodil_152

Daffodil_153

Daffodil_154

Daffodil_155

Daffodil_156

Daffodil_157

Daffodil_158 — tRNA
(tRNA-Pro(tgg))

Daffodil_159 — tRNA
(codon recognized: UGG)

Daffodil_160 — tRNA
(codon recognized: UAC)

Daffodil_161

Daffodil_162
(peptidyl tRNA hydrolase)

Daffodil_163 — tRNA
(tRNA-Pyl(cta))

Daffodil_164 — tRNA
(tRNA-Met (cat))

Daffodil_165 — tRNA
(tRNA-Cys (gca))

Daffodil_166 — tRNA
(tRNA-Glu (ctc))

Daffodil_167 — tRNA
(tRNA-His (gtg))

Daffodil_168 — tRNA
(tRNA-Ala (tgc))

Daffodil_169 — tRNA
(tRNA-Phe (gaa))

Daffodil_170 — tRNA
(tRNA-Val (cac))

Daffodil_171 — tRNA
(tRNA-Lys (ctt))

Daffodil_172 — tRNA
(tRNA-Glu (ttc))

Daffodil_173 — tRNA
(tRNA-Gly (tcc))

Daffodil_174 — tRNA
(tRNA-Thr (cgt))

Daffodil_175 — tRNA
(tRNA-Thr (tgt))

Daffodil_176 — tRNA
(tRNA-Thr (ggt))

Daffodil_177
(minor tail protein)

Daffodil_178

Daffodil_179 — tRNA
(tRNA-Gly (gcc))

Daffodil_180 — tRNA
(tRNA-Asp (gtc))

Daffodil_181 — tRNA
(tRNA-Met (cat))

Daffodil_182 — tRNA
(tRNA-Ile (gat))

Daffodil_183 — tRNA
(tRNA-Arg (acg))

Daffodil_184 — tRNA
(tRNA-Val (gac))

Daffodil_185 — tRNA
(tRNA-Arg (cct))

Daffodil_186 — tmRNA
(tmRNA)

Daffodil_187 — tRNA
(tRNA-Gln (ttg))

Daffodil_188 — tRNA
(tRNA-Arg(tct))

Daffodil_189 — tRNA
(tRNA-Lys(ttt))

Daffodil_190

Daffodil_191

Daffodil_192
(DNA helicase)

Daffodil_193

Daffodil_194

Daffodil_195

Daffodil_196

Daffodil_197

Daffodil_198

Daffodil_199

Daffodil_200

Daffodil_201

Daffodil_202
(thymidylate kinase)

Daffodil_203
(aminotransferase)

Daffodil_204

Daffodil_205

Daffodil_206

Daffodil_207
(DnaC-like helicase loader)

Daffodil_208
(DnaB-like dsDNA helicase)

Daffodil_209

Daffodil_210
(DNA primase)

Daffodil_211

Daffodil_212
(DnaJ-like chaperonin)

Daffodil_213

Daffodil_214
(DnaE-like DNA polymerase III)

Daffodil_215
(RF-1 peptide chain release factor)

Daffodil_216
(RecA-like DNA recombinase)

Daffodil_217

Daffodil_218

Daffodil_219
(HNH endonuclease)

Daffodil_220
(RusA-like resolvase)

Daffodil_221

Daffodil_222

Daffodil_223

Daffodil_224

Daffodil_225

Daffodil_226

Daffodil_227

Daffodil_228 — tRNA
(tRNA-Gln(ctg))

Daffodil_229 — tRNA
(tRNA-Asn(gtt))

Daffodil_230

Daffodil_231

Daffodil_232

Daffodil_233

Daffodil_234

Daffodil_235

Daffodil_236

Daffodil_237

Daffodil_238
(Ro-like RNA binding protein)

Daffodil_239

Daffodil_240

Daffodil_241

Daffodil_242

Daffodil_243

Daffodil_244

Daffodil_245

Daffodil_246
(glycosyltransferase)

Daffodil_247

Daffodil_248
(glycosyltransferase)

Daffodil_249
(glycosyltransferase)

Daffodil_250
(PnuC-like nicotinamide riboside transporter)

Daffodil_251
(glycosyltransferase)

Daffodil_252

Daffodil_253

Daffodil_254

Daffodil_255

Daffodil_256
(lysin A)

Daffodil_257
(holin)

Daffodil_258
(lysin B)

Daffodil_259
(terminase)

Daffodil_260

Daffodil_261

Daffodil_262

Daffodil_263

Daffodil_264
(polynucleotide kinase)

Daffodil_265

Daffodil_266

Daffodil_267
(serine/threonine kinase)

Daffodil_268

Daffodil_269

Daffodil_270
(PurA-like adenylosuccinate synthetase)

Daffodil_271

Daffodil_272

Daffodil_273

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919499
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 10^9
Pitt Freezer Box# 24
Pitt Freezer Box Grid# G3
Available Files
Plaque PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload