Mycobacterium phage Darko
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Detailed Information for Phage Darko
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMegan Majewski & Andrew Chichester
Year Found2017
Location FoundConway, SC USA
Finding InstitutionCoastal Carolina University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.8006 N, 79.0176 W Map
Discovery NotesFound near the dumpster behind Teal Residence Hall at 7:15 am. Clear warm morning. Soil was collected just below the surface.
Naming NotesDarko is the nickname of a friend
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1040
Genome length (bp)155951
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

Lethe (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionCoastal Carolina University
Annotation StatusIn GenBank
Plaque NotesVery small, clear, and tight plaques
MorphotypeMyoviridae
Number of Genes232
Number of tRNAs33
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Darko_1

Darko_2

Darko_3
(HNH endonuclease)

Darko_4

Darko_5

Darko_6

Darko_7

Darko_8

Darko_9

Darko_10

Darko_11

Darko_12

Darko_13

Darko_14

Darko_15

Darko_16

Darko_17
(histidine triad nucleotide binding protein)

Darko_18

Darko_19

Darko_20

Darko_21

Darko_22

Darko_23

Darko_24

Darko_25

Darko_26

Darko_27

Darko_28

Darko_29

Darko_30

Darko_31

Darko_32

Darko_33

Darko_34
(ribbon-helix-helix DNA binding protein)

Darko_35

Darko_36

Darko_37

Darko_38

Darko_39

Darko_40

Darko_41

Darko_42

Darko_43

Darko_44

Darko_45

Darko_46

Darko_47

Darko_48

Darko_49

Darko_50

Darko_51

Darko_52

Darko_53

Darko_54

Darko_55

Darko_56

Darko_57

Darko_58

Darko_59

Darko_60

Darko_61

Darko_62
(helix-turn-helix DNA binding domain protein)

Darko_63
(helix-turn-helix DNA binding protein)

Darko_64

Darko_65

Darko_66

Darko_67

Darko_68

Darko_69

Darko_70

Darko_71

Darko_72

Darko_73

Darko_74

Darko_75

Darko_76

Darko_77

Darko_78

Darko_79

Darko_80

Darko_81

Darko_82

Darko_83

Darko_84 — tRNA
(tRNA-Ser(gct))

Darko_85 — tRNA
(tRNA-Leu(cag))

Darko_86 — tRNA
(tRNA-Leu(gag))

Darko_87 — tRNA
(tRNA-Leu(caa))

Darko_88

Darko_89

Darko_90
(portal protein)

Darko_91

Darko_92

Darko_93
(ThyX-like thymidylate synthase)

Darko_94

Darko_95
(RNA ligase)

Darko_96

Darko_97

Darko_98

Darko_99
(major capsid protein)

Darko_100

Darko_101

Darko_102

Darko_103

Darko_104

Darko_105

Darko_106

Darko_107

Darko_108

Darko_109

Darko_110

Darko_111

Darko_112

Darko_113

Darko_114

Darko_115

Darko_116

Darko_117

Darko_118

Darko_119

Darko_120

Darko_121
(acetyltransferase)

Darko_122

Darko_123

Darko_124

Darko_125

Darko_126

Darko_127
(tail sheath protein)

Darko_128

Darko_129

Darko_130
(tail assembly chaperone)

Darko_131
(tail assembly chaperone)

Darko_132
(tape measure protein)

Darko_133
(minor tail protein)

Darko_134

Darko_135

Darko_136
(minor tail protein)

Darko_137

Darko_138

Darko_139
(baseplate wedge protein)

Darko_140
(baseplate wedge protein)

Darko_141

Darko_142

Darko_143

Darko_144
(minor tail protein)

Darko_145
(minor tail protein)

Darko_146

Darko_147

Darko_148

Darko_149

Darko_150

Darko_151

Darko_152

Darko_153 — tRNA
(tRNA-Pro(tgg))

Darko_154 — tRNA
(tRNA-Trp(cca))

Darko_155 — tRNA
(tRNA-Tyr(gta))

Darko_156

Darko_157
(peptidyl tRNA hydrolase)

Darko_158 — tRNA

Darko_159 — tRNA
(tRNA-Met(cat))

Darko_160 — tRNA
(tRNA-Cys(gca))

Darko_161 — tRNA
(tRNA-Glu(ctc))

Darko_162 — tRNA
(tRNA-His(gtg))

Darko_163 — tRNA
(tRNA-Ala(tgc))

Darko_164 — tRNA
(tRNA-Phe(gaa))

Darko_165 — tRNA
(tRNA-Val(cac))

Darko_166 — tRNA
(tRNA-Lys(ctt))

Darko_167 — tRNA
(tRNA-Glu(ttc))

Darko_168 — tRNA
(tRNA-Gly(tcc))

Darko_169 — tRNA
(tRNA-Thr(cgt))

Darko_170 — tRNA
(tRNA-Thr(tgt))

Darko_171
(HNH endonuclease)

Darko_172 — tRNA
(tRNA-Thr(ggt))

Darko_173
(minor tail protein)

Darko_174

Darko_175 — tRNA
(tRNA-Gly(gcc))

Darko_176 — tRNA
(tRNA-Asp(gtc))

Darko_177 — tRNA
(tRNA-Met(cat))

Darko_178 — tRNA
(tRNA-Ile(gat))

Darko_179 — tRNA
(tRNA-Arg(acg))

Darko_180 — tRNA
(tRNA-Val(gac))

Darko_181 — tRNA
(tRNA-Arg(cct))

Darko_182 — tmRNA
(tmRNA)

Darko_183 — tRNA
(tRNA-Gln(ttg))

Darko_184 — tRNA
(tRNA-Arg(tct))

Darko_185 — tRNA
(tRNA-Lys(ttt))

Darko_186

Darko_187
(DNA helicase)

Darko_188

Darko_189

Darko_190

Darko_191

Darko_192

Darko_193

Darko_194

Darko_195

Darko_196
(thymidylate kinase)

Darko_197
(aminotransferase)

Darko_198

Darko_199

Darko_200

Darko_201
(DnaC-like helicase loader)

Darko_202
(DnaB-like dsDNA helicase)

Darko_203

Darko_204
(DNA primase)

Darko_205

Darko_206
(DnaJ-like chaperonin)

Darko_207
(ssDNA binding protein)

Darko_208
(DnaE-like DNA polymerase III)

Darko_209
(RF-1 peptide chain release factor)

Darko_210
(RecA-like DNA recombinase)

Darko_211
(holliday junction resolvase)

Darko_212

Darko_213
(HNH endonuclease)

Darko_214
(RusA-like resolvase)

Darko_215

Darko_216

Darko_217

Darko_218

Darko_219

Darko_220

Darko_221

Darko_222 — tRNA
(tRNA-Gln(ctg))

Darko_223 — tRNA
(tRNA-Asn(gtt))

Darko_224

Darko_225

Darko_226

Darko_227

Darko_228

Darko_229

Darko_230

Darko_231

Darko_232
(Ro-like RNA binding protein)

Darko_233

Darko_234

Darko_235

Darko_236

Darko_237

Darko_238

Darko_239
(glycosyltransferase)

Darko_240

Darko_241
(glycosyltransferase)

Darko_242
(glycosyltransferase)

Darko_243
(PnuC-like nicotinamide riboside transporter)

Darko_244
(glycosyltransferase)

Darko_245

Darko_246

Darko_247

Darko_248

Darko_249
(HNH endonuclease)

Darko_250
(lysin A)

Darko_251

Darko_252
(lysin B)

Darko_253
(terminase)

Darko_254

Darko_255

Darko_256

Darko_257

Darko_258
(polynucleotide kinase)

Darko_259

Darko_260

Darko_261
(serine/threonine kinase)

Darko_262

Darko_263
(PurA-like adenylosuccinate synthetase)

Darko_264

Darko_265

Darko_266

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK359361
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 62
Pitt Freezer Box Grid# B10
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload