| Detailed Information for Phage Derek |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Barbara Tsaousis |
| Year Found | 2015 |
| Location Found | Bethlehem, PA USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | -1°C |
| GPS Coordinates | 40.6065 N, 75.372273 W Map |
| Discovery Notes | Derek was discovered on Lehigh University's campus. The soil sample was taken from approximately 2 inches beneath the surface. |
| Naming Notes | Named after the fictional character Derek Shepherd, who was a neurosurgeon on ABC's Grey's Anatomy. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 5, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 423 |
| Genome length (bp) | 156199 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were originally very small (approx. 1mm) and turbid when phages were left to infect M. smegmatis at 37 degrees Celsius, but it was discovered that they developed an opaque, shadowy region surrounding each plaque upon spending excessive time at cold temperatures (4 degrees Celsius). This shadowy region was distinct from the plaques and was confirmed by a repeated trial. It was also confirmed that said regions were not present after undergoing regular incubation at 37 degrees. Picture provided is of a plate that was not subjected to colder temperatures. |
| Morphotype | Myoviridae |
| Number of Genes | 229 |
| Number of tRNAs | 32 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH316561 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.60*10^11 pfu/mL |
| Date of SEA Lysate Titering |
Mar 30, 2015 |
| Pitt Freezer Box# |
15 |
| Pitt Freezer Box Grid# |
G5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
