Mycobacterium phage GlutenPhree
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| Detailed Information for Phage GlutenPhree | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kiley Toth |
| Year Found | 2024 |
| Location Found | Merion Station, NJ United States |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 39.99792 N, 75.24171 W Map |
| Discovery Notes | The sample was collected on 09/10/24, at 4:08 PM at the Barnes Arboretum at Saint Joseph’s University, 50 Lapsley Lane, Merion Station, PA 19066. The sample was collected under a bush near a sidewalk, with sunny weather conditions present. There was likely little foot traffic present. The soil was dry, and the depth was approximately 5 cm deep. The ambient temperature was 27.2222 ⁰C. The ambient humidity was 41% , and there were sunny weather conditions present. |
| Naming Notes | GlutenPhree was named after one of my lab partners, Nora Duval. Nora and I had collected our soil samples together. Before every biology or chemistry exam, Nora and I also studied together while eating gluten free muffins. Given my love for the prefix ph-, the name GlutenPhree was created. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Phage yields clear plaques approximately 1mm in size after a 48 hour incubation period. Plaques have not been consistent in size despite multiple rounds of purification, with some plaques appearing larger than 1mm, and others significantly smaller. This variation is assumed to be characteristic of the phage. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 5.5 x 10^9 pfu/mL |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |