The Actinobacteriophage Database

Mycobacterium phage GlutenPhree

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Detailed Information for Phage GlutenPhree
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Kiley Toth
Year Found	2024
Location Found	Merion Station, PA United States
Finding Institution	Saint Joseph's University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	39.99792 N, 75.24171 W Map
Discovery Notes	The sample was collected on 09/10/24, at 4:08 PM at the Barnes Arboretum at Saint Joseph’s University, 50 Lapsley Lane, Merion Station, PA 19066. The sample was collected under a bush near a sidewalk, with sunny weather conditions present. There was likely little foot traffic present. The soil was dry, and the depth was approximately 5 cm deep. The ambient temperature was 27.2222 ⁰C. The ambient humidity was 41% , and there were sunny weather conditions present.
Naming Notes	GlutenPhree was named after one of my lab partners, Nora Duval. Nora and I had collected our soil samples together. Before every biology or chemistry exam, Nora and I also studied together while eating gluten free muffins. Given my love for the prefix ph-, the name GlutenPhree was created.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 10, 2025
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina NextSeq 1000
Read Type	Single-end reads
Read Length	100 bp
Approximate Shotgun Coverage	1486
Genome length (bp)	154554
Character of genome ends	Circularly Permuted
GC Content	64.7%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	C
Subcluster	C1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Ablatia (C1) Ading (C1) Adlitam (C1) Alice (C1) Amataga (C1) ArcherS7 (C1) Asterius (C1) Astraea (C1) Atlantean (C1) Audrick (C1) Ava3 (C1) Babyland (C1) BackyardAgain (C1) BadAgartude (C1) BananaFence (C1) Bangla1971 (C1) Basquiat (C1) BeanWater (C1) Belieber (C1) BigCity (C1) Bigswole (C1) Bipolarisk (C1) Blackbrain (C1) Blackdragon (C1) Blubba (C1) Bonray (C1) Bread (C1) Breeniome (C1) Burrough (C1) Bxz1 (C1) Cactojaque (C1) Cali (C1) Cane17 (C1) Capablanca (C1) Caravan (C1) Catera (C1) Chargie21 (C1) CharlieB (C1) ChaylaJr (C1) ChickenPhender (C1) Chomp (C1) CindyLou (C1) Colt (C1) Concombre (C1) Daffodil (C1) Dandelion (C1) Darko (C1) DeejaClivia (C1) Delilah (C1) Delylah (C1) Demodog (C1) Derek (C1) Dietrick (C1) DirtMonster (C1) Domi (C1) Drazdys (C1) DrPhinkDaddy (C1) DTDevon (C1) EasyJones (C1) EggyFarm (C1) EmmaElysia (C1) EmToTheThree (C1) Erdmann (C1) ErnieJ (C1) Essence (C1) ET08 (C1) Ewok (C1) Flabslab (C1) Fludd (C1) Fongie (C1) FoxtrotP1 (C1) FrayBell (C1) FudgeTart (C1) Gabriel (C1) Gamgam (C1) Ghost (C1) Gizmo (C1) GlutenPhree (C1) Grasshills (C1) Grungle (C1) Guwapp (C1) Halldule (C1) HyRo (C1) I3 (C1) IkeLoa (C1) InigoMontoya (C1) InterFolia (C1) Iota (C1) Issimir (C1) Izajani (C1) Janiyra (C1) Jaygup (C1) JayJay (C1) Jedediah (C1) Jemmno (C1) Jessibeth14 (C1) Jezreel (C1) JigglyPuff (C1) JPickles (C1) JulietS (C1) JustHall (C1) Kamryn (C1) Kboogie (C1) Khaleesi (C1) Koguma (C1) Labradorite (C1) Latch (C1) Lee7 (C1) Lethe (C1) LifeSavor (C1) LinStu (C1) Littleton (C1) Loanshark (C1) LolaVinca (C1) LordLeafolot (C1) LRRHood (C1) Lukilu (C1) Maby2021 (C1) Mangeria (C1) Matsumoto (C1) McGee (C1) McWolfish (C1) Megamind (C1) Melpomini (C1) MikeLiesIn (C1) Mikro (C1) Momo (C1) MoMoMixon (C1) Morizzled23 (C1) Naija (C1) Napoleon13 (C1) Nappy (C1) Naval22 (C1) NedWong (C1) Ngannou (C1) Nidhogg (C1) NoodleTree (C1) Norm (C1) NuevoMundo (C1) ParkTD (C1) Patter (C1) Phlegm (C1) Phox (C1) Phusco (C1) Pier (C1) Pinkcreek (C1) Pio (C1) Pivoine (C1) Pleione (C1) Primadonna (C1) PurduePete (C1) QBert (C1) Quasimodo (C1) Rabinovish (C1) Rahel (C1) Rizal (C1) RoMag (C1) Ronan (C1) Roots515 (C1) Salacia (C1) Sauce (C1) ScottMcG (C1) Sebata (C1) Shaqnato (C1) Shelob (C1) ShiaLabeouf (C1) Shifa (C1) Shnickers (C1) Shrimp (C1) SilverDipper (C1) SmallFry (C1) Spec (C1) Specks (C1) Sprinklers (C1) Spud (C1) SrishMeg2525 (C1) StephanieG (C1) Stubby (C1) StubbySaigey (C1) SVoro (C1) Tainan (C1) Teardrop (C1) TinyTim (C1) Tonenili (C1) Tortoise16 (C1) Trinitium (C1) Turret (C1) Tyke (C1) ValleyTerrace (C1) Wally (C1) Willis (C1) Yassified (C1) YemiJoy2021 (C1) YoungMoneyMata (C1) Yucca (C1) Zalkecks (C1) Zeenon (C1) ZygoTaiga (C1) Greely (C2) Myrna (C2) Phabba (C2) ScoobyDoobyDoo (C2)
Annotating Institution	Saint Joseph's University
Annotation Status	QC Complete
Plaque Notes	Phage yields clear plaques approximately 1mm in size after a 48 hour incubation period. Plaques have not been consistent in size despite multiple rounds of purification, with some plaques appearing larger than 1mm, and others significantly smaller. This variation is assumed to be characteristic of the phage.
Morphotype	Myoviridae
Number of Genes	231
Number of tRNAs	33
Number of tmRNAs	1
Has been Phamerated?	Yes
Gene List	Click to View GlutenPhree_1 GlutenPhree_2 GlutenPhree_3 GlutenPhree_4 GlutenPhree_5 GlutenPhree_6 GlutenPhree_7 GlutenPhree_8 GlutenPhree_9 GlutenPhree_10 GlutenPhree_11 GlutenPhree_12 GlutenPhree_13 GlutenPhree_14 GlutenPhree_15 GlutenPhree_16 GlutenPhree_17 GlutenPhree_18 GlutenPhree_19 GlutenPhree_20 GlutenPhree_21 GlutenPhree_22 GlutenPhree_23 GlutenPhree_24 GlutenPhree_25 GlutenPhree_26 GlutenPhree_27 GlutenPhree_28 GlutenPhree_29 GlutenPhree_30 GlutenPhree_31 GlutenPhree_32 GlutenPhree_33 GlutenPhree_34 GlutenPhree_35 GlutenPhree_36 GlutenPhree_37 GlutenPhree_38 GlutenPhree_39 GlutenPhree_40 GlutenPhree_41 GlutenPhree_42 GlutenPhree_43 GlutenPhree_44 GlutenPhree_45 GlutenPhree_46 GlutenPhree_47 GlutenPhree_48 GlutenPhree_49 GlutenPhree_50 GlutenPhree_51 GlutenPhree_52 GlutenPhree_53 GlutenPhree_54 GlutenPhree_55 GlutenPhree_56 GlutenPhree_57 GlutenPhree_58 GlutenPhree_59 GlutenPhree_60 GlutenPhree_61 GlutenPhree_62 GlutenPhree_63 GlutenPhree_64 GlutenPhree_65 GlutenPhree_66 GlutenPhree_67 GlutenPhree_68 GlutenPhree_69 GlutenPhree_70 GlutenPhree_71 GlutenPhree_72 GlutenPhree_73 GlutenPhree_74 GlutenPhree_75 GlutenPhree_76 GlutenPhree_77 GlutenPhree_78 GlutenPhree_79 GlutenPhree_80 GlutenPhree_81 GlutenPhree_82 — tRNA (gp82) GlutenPhree_83 — tRNA (gp83) GlutenPhree_84 — tRNA (gp84) GlutenPhree_85 — tRNA (gp85) GlutenPhree_86 GlutenPhree_87 GlutenPhree_88 GlutenPhree_89 GlutenPhree_90 GlutenPhree_91 GlutenPhree_92 GlutenPhree_93 GlutenPhree_94 GlutenPhree_95 GlutenPhree_96 GlutenPhree_97 GlutenPhree_98 GlutenPhree_99 GlutenPhree_100 GlutenPhree_101 GlutenPhree_102 GlutenPhree_103 GlutenPhree_104 GlutenPhree_105 GlutenPhree_106 GlutenPhree_107 GlutenPhree_108 GlutenPhree_109 GlutenPhree_110 GlutenPhree_111 GlutenPhree_112 GlutenPhree_113 GlutenPhree_114 GlutenPhree_115 GlutenPhree_116 GlutenPhree_117 GlutenPhree_118 GlutenPhree_119 GlutenPhree_120 GlutenPhree_121 GlutenPhree_122 GlutenPhree_123 GlutenPhree_124 GlutenPhree_125 GlutenPhree_126 GlutenPhree_127 — tRNA (gp127) GlutenPhree_128 GlutenPhree_129 GlutenPhree_130 GlutenPhree_131 GlutenPhree_132 GlutenPhree_133 GlutenPhree_134 GlutenPhree_135 GlutenPhree_136 GlutenPhree_137 GlutenPhree_138 GlutenPhree_139 GlutenPhree_140 GlutenPhree_141 GlutenPhree_142 GlutenPhree_143 GlutenPhree_144 GlutenPhree_145 GlutenPhree_146 GlutenPhree_147 GlutenPhree_148 GlutenPhree_149 GlutenPhree_150 GlutenPhree_151 GlutenPhree_152 GlutenPhree_153 — tRNA (gp153) GlutenPhree_154 — tRNA (gp154) GlutenPhree_155 — tRNA (gp155) GlutenPhree_156 GlutenPhree_157 GlutenPhree_158 — tRNA (gp158) GlutenPhree_159 — tRNA (gp159) GlutenPhree_160 — tRNA (gp160) GlutenPhree_161 — tRNA (gp161) GlutenPhree_162 — tRNA (gp162) GlutenPhree_163 — tRNA (gp163) GlutenPhree_164 GlutenPhree_165 — tRNA (gp165) GlutenPhree_166 — tRNA (gp166) GlutenPhree_167 — tRNA (gp167) GlutenPhree_168 — tRNA (gp168) GlutenPhree_169 — tRNA (gp169) GlutenPhree_170 — tRNA (gp170) GlutenPhree_171 — tRNA (gp171) GlutenPhree_172 GlutenPhree_173 — tRNA (gp173) GlutenPhree_174 GlutenPhree_175 GlutenPhree_176 — tRNA (gp176) GlutenPhree_177 — tRNA (gp177) GlutenPhree_178 — tRNA (gp178) GlutenPhree_179 — tRNA (gp179) GlutenPhree_180 — tRNA (gp180) GlutenPhree_181 — tRNA (gp181) GlutenPhree_182 — tRNA (gp182) GlutenPhree_183 — tmRNA (gp183) GlutenPhree_184 — tRNA (gp184) GlutenPhree_185 — tRNA (gp185) GlutenPhree_186 GlutenPhree_187 GlutenPhree_188 GlutenPhree_189 GlutenPhree_190 GlutenPhree_191 GlutenPhree_192 GlutenPhree_193 GlutenPhree_194 GlutenPhree_195 GlutenPhree_196 GlutenPhree_197 GlutenPhree_198 GlutenPhree_199 GlutenPhree_200 GlutenPhree_201 GlutenPhree_202 GlutenPhree_203 GlutenPhree_204 GlutenPhree_205 GlutenPhree_206 GlutenPhree_207 GlutenPhree_208 GlutenPhree_209 GlutenPhree_210 GlutenPhree_211 GlutenPhree_212 GlutenPhree_213 GlutenPhree_214 GlutenPhree_215 GlutenPhree_216 GlutenPhree_217 GlutenPhree_218 GlutenPhree_219 GlutenPhree_220 GlutenPhree_221 GlutenPhree_222 GlutenPhree_223 — tRNA (gp223) GlutenPhree_224 — tRNA (gp224) GlutenPhree_225 GlutenPhree_226 GlutenPhree_227 GlutenPhree_228 GlutenPhree_229 GlutenPhree_230 GlutenPhree_231 GlutenPhree_232 GlutenPhree_233 GlutenPhree_234 GlutenPhree_235 GlutenPhree_236 GlutenPhree_237 GlutenPhree_238 GlutenPhree_239 GlutenPhree_240 GlutenPhree_241 GlutenPhree_242 GlutenPhree_243 GlutenPhree_244 GlutenPhree_245 GlutenPhree_246 GlutenPhree_247 GlutenPhree_248 GlutenPhree_249 GlutenPhree_250 GlutenPhree_251 GlutenPhree_252 GlutenPhree_253 GlutenPhree_254 GlutenPhree_255 GlutenPhree_256 GlutenPhree_257 GlutenPhree_258 GlutenPhree_259 GlutenPhree_260 GlutenPhree_261 GlutenPhree_262 GlutenPhree_263 GlutenPhree_264 GlutenPhree_265
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	No
GenBank Accession	None yet
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Lysate Titer	5.5 x 10^9 pfu/mL
Pitt Freezer Box#	212
Pitt Freezer Box Grid#	H8
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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