Mycobacterium phage Pleione
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Detailed Information for Phage Pleione
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGrace Kroner and Emily Lazowick
Year Found2010
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.643594 N, 90.298122 W Map
Discovery NotesAbout 1 cm. below surface; soil was moist; urban environment; no buildings; some foot traffic; no nearby roads; no cement; water at the bottom of the hill; trees surrounding the area; on a north facing slope; harvested by a decomposing tree; 24 degrees Celsius; harvested on 9/3/2010 at 4:57 pm.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 24, 2011
Genome length (bp)155586
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesVery small plaques, usually smaller than 1mm in diameter; clear plaques; no variation.
MorphotypeMyoviridae
Number of Genes236
Number of tRNAs35
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Pleione_1

Pleione_2

Pleione_3
(nucleotidyltransferase)

Pleione_4

Pleione_5

Pleione_6

Pleione_7

Pleione_8

Pleione_9

Pleione_10

Pleione_11

Pleione_12

Pleione_13

Pleione_14

Pleione_15

Pleione_16

Pleione_17

Pleione_18

Pleione_19

Pleione_20
(histidine triad nucleotide binding protein)

Pleione_21

Pleione_22

Pleione_23

Pleione_24

Pleione_25

Pleione_26

Pleione_27

Pleione_28

Pleione_29

Pleione_30

Pleione_31
(band-7-like membrane protein)

Pleione_32

Pleione_33

Pleione_34

Pleione_35

Pleione_36

Pleione_37

Pleione_38

Pleione_39

Pleione_40

Pleione_41

Pleione_42

Pleione_43

Pleione_44

Pleione_45

Pleione_46

Pleione_47

Pleione_48

Pleione_49

Pleione_50

Pleione_51

Pleione_52

Pleione_53

Pleione_54

Pleione_55

Pleione_56

Pleione_57

Pleione_58

Pleione_59

Pleione_60

Pleione_61

Pleione_62

Pleione_63

Pleione_64

Pleione_65
(helix-turn-helix DNA binding domain protein)

Pleione_66

Pleione_67

Pleione_68

Pleione_69

Pleione_70

Pleione_71

Pleione_72

Pleione_73

Pleione_74

Pleione_75

Pleione_76

Pleione_77

Pleione_78

Pleione_79

Pleione_80

Pleione_81

Pleione_82

Pleione_83

Pleione_84

Pleione_85

Pleione_86 — tRNA
(tRNA-Ser (gct))

Pleione_87 — tRNA
(tRNA-Ser (cga))

Pleione_88 — tRNA
(tRNA-Leu (cag))

Pleione_89 — tRNA
(tRNA-Leu (gag))

Pleione_90 — tRNA
(tRNA-Leu (caa))

Pleione_91

Pleione_92

Pleione_93
(portal protein)

Pleione_94

Pleione_95

Pleione_96
(ThyX-like thymidylate synthase)

Pleione_97

Pleione_98
(RNA ligase)

Pleione_99

Pleione_100

Pleione_101

Pleione_102
(major capsid protein)

Pleione_103

Pleione_104

Pleione_105

Pleione_106

Pleione_107

Pleione_108

Pleione_109

Pleione_110

Pleione_111

Pleione_112

Pleione_113

Pleione_114

Pleione_115

Pleione_116

Pleione_117

Pleione_118

Pleione_119

Pleione_120

Pleione_121

Pleione_122

Pleione_123

Pleione_124

Pleione_125

Pleione_126

Pleione_127

Pleione_128

Pleione_129

Pleione_130
(tail sheath protein)

Pleione_131

Pleione_132

Pleione_134
(tail assembly chaperone)

Pleione_133
(tail assembly chaperone)

Pleione_135
(tape measure protein)

Pleione_136

Pleione_137

Pleione_138

Pleione_139
(minor tail protein)

Pleione_140

Pleione_141
(minor tail protein)

Pleione_142

Pleione_143
(baseplate J protein)

Pleione_144

Pleione_145

Pleione_146

Pleione_147

Pleione_148
(minor tail protein)

Pleione_149

Pleione_150

Pleione_151

Pleione_152

Pleione_153

Pleione_154

Pleione_155

Pleione_156 — tRNA
(tRNA-Pro (tgg))

Pleione_157 — tRNA
(tRNA-Trp (cca))

Pleione_158 — tRNA
(tRNA-Tyr (gta))

Pleione_159

Pleione_160
(peptidyl tRNA hydrolase)

Pleione_161 — tRNA
(tRNA-other (cta))

Pleione_162 — tRNA
(tRNA-Met (cat))

Pleione_163 — tRNA
(tRNA-Cys (gca))

Pleione_164 — tRNA
(tRNA-Glu (ctc))

Pleione_165 — tRNA
(tRNA-His (gtg))

Pleione_166 — tRNA
(tRNA-Ala (tgc))

Pleione_167 — tRNA
(tRNA-Phe (gaa))

Pleione_168 — tRNA
(tRNA-Val (cac))

Pleione_169 — tRNA
(tRNA-Lys (ctt))

Pleione_170 — tRNA
(tRNA-Glu (ttc))

Pleione_171 — tRNA
(tRNA-Gly (tcc))

Pleione_172 — tRNA
(tRNA-Thr (cgt))

Pleione_173 — tRNA
(tRNA-Thr (tgt))

Pleione_174
(HNH endonuclease)

Pleione_175 — tRNA
(tRNA-Thr (ggt))

Pleione_176
(minor tail protein)

Pleione_177

Pleione_178 — tRNA
(tRNA-Gly (gcc))

Pleione_179 — tRNA
(tRNA-Asp (gtc))

Pleione_180 — tRNA
(tRNA-Met (cat))

Pleione_181 — tRNA
(tRNA-Ile (gat))

Pleione_182 — tRNA
(tRNA-Arg (acg))

Pleione_183 — tRNA
(tRNA-Val (gac))

Pleione_184 — tRNA
(tRNA-Arg (ccg))

Pleione_185 — tRNA
(tRNA-Arg (cct))

Pleione_186 — tmRNA

Pleione_187 — tRNA
(tRNA-Gln (ttg))

Pleione_188 — tRNA
(tRNA-Arg (tct))

Pleione_189 — tRNA
(tRNA-Lys (ttt))

Pleione_190

Pleione_191

Pleione_192
(DNA helicase)

Pleione_193

Pleione_194

Pleione_195

Pleione_196

Pleione_197

Pleione_198

Pleione_199

Pleione_200

Pleione_201

Pleione_202

Pleione_203
(aminotransferase)

Pleione_204

Pleione_205

Pleione_206
(DnaC-like helicase loader)

Pleione_207
(DnaB-like dsDNA helicase)

Pleione_208

Pleione_209
(DNA primase)

Pleione_210

Pleione_211
(DnaJ-like chaperonin)

Pleione_212

Pleione_213
(DnaE-like DNA polymerase III)

Pleione_214
(RF-1 peptide chain release factor)

Pleione_215
(RecA-like DNA recombinase)

Pleione_216

Pleione_217

Pleione_218
(HNH endonuclease)

Pleione_219
(RusA-like resolvase)

Pleione_220

Pleione_221

Pleione_222

Pleione_223

Pleione_224

Pleione_225

Pleione_226

Pleione_227 — tRNA
(tRNA-Gln (ctg))

Pleione_228 — tRNA
(tRNA-Asn (gtt))

Pleione_229

Pleione_230

Pleione_231

Pleione_232

Pleione_233

Pleione_234

Pleione_235

Pleione_236

Pleione_237
(Ro-like RNA binding protein)

Pleione_238

Pleione_239

Pleione_240

Pleione_241

Pleione_242

Pleione_243

Pleione_244
(glycosyltransferase)

Pleione_245

Pleione_246
(glycosyltransferase)

Pleione_247
(glycosyltransferase)

Pleione_248
(PnuC-like nicotinamide riboside transporter)

Pleione_249
(glycosyltransferase)

Pleione_250

Pleione_251

Pleione_252

Pleione_253

Pleione_254
(lysin A)

Pleione_255
(holin)

Pleione_256
(lysin B)

Pleione_257
(terminase)

Pleione_258

Pleione_259

Pleione_260

Pleione_261

Pleione_262
(polynucleotide kinase)

Pleione_263

Pleione_264

Pleione_265
(serine/threonine kinase)

Pleione_266

Pleione_267

Pleione_268
(PurA-like adenylosuccinate synthetase)

Pleione_269

Pleione_270

Pleione_271

Pleione_272

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionJN624850
Refseq NumberNC_023737
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator WUSL
Pitt Freezer Box# 20
Pitt Freezer Box Grid# 31
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload