The Actinobacteriophage Database

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Detailed Information for Phage Rocha
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	IR, BW, MP, JM, DF, AJ, MM, KT
Year Found	2025
Location Found	Austin, TX United States
Finding Institution	Austin Community College Eastview Campus
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	30.14684 N, 97.75341 W Map
Discovery Notes	Isabela Pereira da Rocha, Bryce Wasem, Missy Palacios, Jayden Mullen, Daeza Fowler, Andrew Jinks, Miguel Macias, Kissaou Tchedre. Sample collected from mulch.
Naming Notes	Named after group leader.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jul 11, 2025
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina NextSeq 1000
Read Type	Single-end reads
Read Length	300 bp
Approximate Shotgun Coverage	1730
Genome length (bp)	155112
Character of genome ends	3' Sticky Overhang
GC Content	64.8%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	C
Subcluster	C1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Ablatia (C1) Ading (C1) Adlitam (C1) Alice (C1) Amataga (C1) ArcherS7 (C1) Asterius (C1) Astraea (C1) Atlantean (C1) Audrick (C1) Ava3 (C1) Babyland (C1) BackyardAgain (C1) BadAgartude (C1) BananaFence (C1) Bangla1971 (C1) Basquiat (C1) BeanWater (C1) Belieber (C1) Bezza (C1) BigCity (C1) Bigswole (C1) Bipolarisk (C1) Blackbrain (C1) Blackdragon (C1) Blubba (C1) Bonray (C1) Bread (C1) Breeniome (C1) Burrough (C1) Bxz1 (C1) Cactojaque (C1) Cali (C1) Cane17 (C1) Capablanca (C1) Caravan (C1) Catera (C1) Chargie21 (C1) CharlieB (C1) ChaylaJr (C1) ChickenPhender (C1) Chomp (C1) CindyLou (C1) Colt (C1) Concombre (C1) Daffodil (C1) Dandelion (C1) Darko (C1) DeejaClivia (C1) Delilah (C1) Delylah (C1) Demodog (C1) Derek (C1) Dietrick (C1) DirtMonster (C1) Domi (C1) Drazdys (C1) DrPhinkDaddy (C1) DTDevon (C1) EasyJones (C1) EggyFarm (C1) EmmaElysia (C1) EmToTheThree (C1) Erdmann (C1) ErnieJ (C1) Essence (C1) ET08 (C1) Ewok (C1) Flabslab (C1) Fludd (C1) Fongie (C1) FoxtrotP1 (C1) FrayBell (C1) FudgeTart (C1) Gabriel (C1) Gamgam (C1) Ghost (C1) Gizmo (C1) GlutenPhree (C1) Grasshills (C1) Grungle (C1) Guwapp (C1) Halldule (C1) HyRo (C1) I3 (C1) IkeLoa (C1) InigoMontoya (C1) InterFolia (C1) Iota (C1) Issimir (C1) Izajani (C1) Janiyra (C1) Jaygup (C1) JayJay (C1) Jedediah (C1) Jemmno (C1) Jessibeth14 (C1) Jezreel (C1) JigglyPuff (C1) JPickles (C1) JulietS (C1) JustHall (C1) Kamryn (C1) Kboogie (C1) Khaleesi (C1) Koguma (C1) Labradorite (C1) Latch (C1) Lee7 (C1) Lethe (C1) LifeSavor (C1) LinStu (C1) Littleton (C1) Loanshark (C1) LolaVinca (C1) LookingGlass (C1) LordLeafolot (C1) LRRHood (C1) Lukilu (C1) Maby2021 (C1) Mangeria (C1) Matsumoto (C1) McGee (C1) McWolfish (C1) Megamind (C1) Melpomini (C1) MikeLiesIn (C1) Mikro (C1) Momo (C1) MoMoMixon (C1) Morizzled23 (C1) Naija (C1) Napoleon13 (C1) Nappy (C1) Naval22 (C1) NedWong (C1) Ngannou (C1) Nidhogg (C1) NoodleTree (C1) Norm (C1) NuevoMundo (C1) OrediggerDelux (C1) ParkTD (C1) Patter (C1) Phlegm (C1) Phox (C1) Phusco (C1) Pier (C1) Pinkcreek (C1) Pio (C1) Pivoine (C1) Pleione (C1) Primadonna (C1) PurduePete (C1) QBert (C1) Quasimodo (C1) Rabinovish (C1) Rahel (C1) Rizal (C1) Rocha (C1) RoMag (C1) Ronan (C1) Roots515 (C1) Salacia (C1) Sauce (C1) ScottMcG (C1) Sebata (C1) Shaqnato (C1) Shelob (C1) ShiaLabeouf (C1) Shifa (C1) Shnickers (C1) Shrimp (C1) SilverDipper (C1) SmallFry (C1) Spec (C1) Specks (C1) Sprinklers (C1) Spud (C1) SrishMeg2525 (C1) StephanieG (C1) Stubby (C1) StubbySaigey (C1) SVoro (C1) Tainan (C1) Teardrop (C1) TinyTim (C1) Tonenili (C1) Tortoise16 (C1) Trinitium (C1) Turret (C1) Tyke (C1) ValleyTerrace (C1) Wally (C1) Willis (C1) Yassified (C1) YemiJoy2021 (C1) YoungMoneyMata (C1) Yucca (C1) Zalkecks (C1) Zeenon (C1) ZygoTaiga (C1) Greely (C2) Myrna (C2) Phabba (C2) ScoobyDoobyDoo (C2)
Annotating Institution	Austin Community College Eastview Campus
Annotation Status	Being Annotated (Expected completion by 12/31/2025)
Plaque Notes	Plaques were mostly tiny, circular, and identical, measuring approximately two millimeters in diameter.
Morphotype	Siphoviridae
Number of Genes	230
Number of tRNAs	32
Number of tmRNAs	1
Has been Phamerated?	Yes
Gene List	Click to View Rocha_1 Rocha_2 Rocha_3 Rocha_4 Rocha_5 Rocha_6 Rocha_7 Rocha_8 Rocha_9 Rocha_10 Rocha_11 Rocha_12 Rocha_13 Rocha_14 Rocha_15 Rocha_16 Rocha_17 Rocha_18 Rocha_19 Rocha_20 Rocha_21 Rocha_22 Rocha_23 Rocha_24 Rocha_25 Rocha_26 Rocha_27 Rocha_28 Rocha_29 Rocha_30 Rocha_31 Rocha_32 Rocha_33 Rocha_34 Rocha_35 Rocha_36 Rocha_37 Rocha_38 Rocha_39 Rocha_40 Rocha_41 Rocha_42 Rocha_43 Rocha_44 Rocha_45 Rocha_46 Rocha_47 Rocha_48 Rocha_49 Rocha_50 Rocha_51 Rocha_52 Rocha_53 Rocha_54 Rocha_55 Rocha_56 Rocha_57 Rocha_58 Rocha_59 Rocha_60 Rocha_61 Rocha_62 Rocha_63 Rocha_64 Rocha_65 Rocha_66 Rocha_67 Rocha_68 Rocha_69 Rocha_70 Rocha_71 Rocha_72 Rocha_73 Rocha_74 Rocha_75 Rocha_76 Rocha_77 Rocha_78 Rocha_79 Rocha_80 Rocha_81 Rocha_82 Rocha_83 — tRNA (gp83) Rocha_84 — tRNA (gp84) Rocha_85 — tRNA (gp85) Rocha_86 — tRNA (gp86) Rocha_87 Rocha_88 Rocha_89 Rocha_90 Rocha_91 Rocha_92 Rocha_93 Rocha_94 Rocha_95 Rocha_96 Rocha_97 Rocha_98 Rocha_99 Rocha_100 Rocha_101 Rocha_102 Rocha_103 Rocha_104 Rocha_105 Rocha_106 Rocha_107 Rocha_108 Rocha_109 Rocha_110 Rocha_111 Rocha_112 Rocha_113 Rocha_114 Rocha_115 Rocha_116 Rocha_117 Rocha_118 Rocha_119 Rocha_120 Rocha_121 Rocha_122 Rocha_123 Rocha_124 Rocha_125 Rocha_126 Rocha_127 Rocha_128 Rocha_129 Rocha_130 Rocha_131 Rocha_132 Rocha_133 Rocha_134 Rocha_135 Rocha_136 Rocha_137 Rocha_138 Rocha_139 Rocha_140 Rocha_141 Rocha_142 Rocha_143 Rocha_144 Rocha_145 Rocha_146 Rocha_147 Rocha_148 Rocha_149 Rocha_150 Rocha_151 — tRNA (gp151) Rocha_152 — tRNA (gp152) Rocha_153 — tRNA (gp153) Rocha_154 Rocha_155 Rocha_156 — tRNA (gp156) Rocha_157 — tRNA (gp157) Rocha_158 — tRNA (gp158) Rocha_159 — tRNA (gp159) Rocha_160 — tRNA (gp160) Rocha_161 — tRNA (gp161) Rocha_162 Rocha_163 — tRNA (gp163) Rocha_164 — tRNA (gp164) Rocha_165 — tRNA (gp165) Rocha_166 — tRNA (gp166) Rocha_167 — tRNA (gp167) Rocha_168 — tRNA (gp168) Rocha_169 — tRNA (gp169) Rocha_170 Rocha_171 — tRNA (gp171) Rocha_172 Rocha_173 Rocha_174 — tRNA (gp174) Rocha_175 — tRNA (gp175) Rocha_176 — tRNA (gp176) Rocha_177 — tRNA (gp177) Rocha_178 — tRNA (gp178) Rocha_179 — tRNA (gp179) Rocha_180 — tRNA (gp180) Rocha_181 — tmRNA (gp181) Rocha_182 — tRNA (gp182) Rocha_183 — tRNA (gp183) Rocha_184 Rocha_185 Rocha_186 Rocha_187 Rocha_188 Rocha_189 Rocha_190 Rocha_191 Rocha_192 Rocha_193 Rocha_194 Rocha_195 Rocha_196 Rocha_197 Rocha_198 Rocha_199 Rocha_200 Rocha_201 Rocha_202 Rocha_203 Rocha_204 Rocha_205 Rocha_206 Rocha_207 Rocha_208 Rocha_209 Rocha_210 Rocha_211 Rocha_212 Rocha_213 Rocha_214 Rocha_215 Rocha_216 Rocha_217 Rocha_218 Rocha_219 Rocha_220 — tRNA (gp220) Rocha_221 — tRNA (gp221) Rocha_222 Rocha_223 Rocha_224 Rocha_225 Rocha_226 Rocha_227 Rocha_228 Rocha_229 Rocha_230 Rocha_231 Rocha_232 Rocha_233 Rocha_234 Rocha_235 Rocha_236 Rocha_237 Rocha_238 Rocha_239 Rocha_240 Rocha_241 Rocha_242 Rocha_243 Rocha_244 Rocha_245 Rocha_246 Rocha_247 Rocha_248 Rocha_249 Rocha_250 Rocha_251 Rocha_252 Rocha_253 Rocha_254 Rocha_255 Rocha_256 Rocha_257 Rocha_258 Rocha_259 Rocha_260 Rocha_261 Rocha_262 Rocha_263
Publication Info
Uploaded to GenBank?	No
GenBank Accession	None yet
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	216
Pitt Freezer Box Grid#	H8
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download

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