Mycobacterium phage SrishMeg2525
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Detailed Information for Phage SrishMeg2525
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMegha Manjunath; Srishti Sharma
Year Found2015
Location FoundWest Lafayette, IN USA
Finding InstitutionPurdue University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.4226 N, 86.9246 W Map
Discovery NotesMoist soil, warm temperatures (70s), light rain two nights before. Spot located 1 ft away from the sidewalk. Found in the soil outside of the Purdue Discovery Learning Resource Center.
In the Fall of 2022, Josh Mariani, Emma Kane, Grace Andrews, David Kim amplified the phage to image.
Naming NotesThe founders first names and a lucky string of numbers.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 13, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage369
Genome length (bp)155718
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionPurdue University
Annotation StatusIn GenBank
Plaque NotesStreaks were often clear and plaques formed nicely in similar sizes.
MorphotypeMyoviridae
Number of Genes231
Number of tRNAs32
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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SrishMeg2525_1

SrishMeg2525_2

SrishMeg2525_3
(nucleotidyl transferase)

SrishMeg2525_4

SrishMeg2525_5

SrishMeg2525_6

SrishMeg2525_7

SrishMeg2525_8

SrishMeg2525_9

SrishMeg2525_10

SrishMeg2525_11

SrishMeg2525_12

SrishMeg2525_13

SrishMeg2525_14

SrishMeg2525_15

SrishMeg2525_16

SrishMeg2525_17

SrishMeg2525_18
(histidine triad nucleotide binding protein)

SrishMeg2525_19

SrishMeg2525_20

SrishMeg2525_21

SrishMeg2525_22

SrishMeg2525_23

SrishMeg2525_24

SrishMeg2525_25

SrishMeg2525_26

SrishMeg2525_27

SrishMeg2525_28
(band-7-like membrane protein)

SrishMeg2525_29

SrishMeg2525_30

SrishMeg2525_31

SrishMeg2525_32

SrishMeg2525_33

SrishMeg2525_34
(ribbon-helix-helix DNA binding domain protein)

SrishMeg2525_35

SrishMeg2525_36

SrishMeg2525_37

SrishMeg2525_38

SrishMeg2525_39

SrishMeg2525_40

SrishMeg2525_41

SrishMeg2525_42

SrishMeg2525_43

SrishMeg2525_44

SrishMeg2525_45

SrishMeg2525_46

SrishMeg2525_47

SrishMeg2525_48

SrishMeg2525_49

SrishMeg2525_50

SrishMeg2525_51

SrishMeg2525_52

SrishMeg2525_53

SrishMeg2525_54

SrishMeg2525_55

SrishMeg2525_56

SrishMeg2525_57

SrishMeg2525_58

SrishMeg2525_59

SrishMeg2525_60

SrishMeg2525_61

SrishMeg2525_62
(helix-turn-helix DNA binding domain protein)

SrishMeg2525_63
(helix-turn-helix DNA binding domain protein)

SrishMeg2525_64

SrishMeg2525_65

SrishMeg2525_66

SrishMeg2525_67

SrishMeg2525_68

SrishMeg2525_69

SrishMeg2525_70

SrishMeg2525_71

SrishMeg2525_72

SrishMeg2525_73

SrishMeg2525_74

SrishMeg2525_75

SrishMeg2525_76

SrishMeg2525_77

SrishMeg2525_78

SrishMeg2525_79

SrishMeg2525_80

SrishMeg2525_81

SrishMeg2525_82

SrishMeg2525_83 — tRNA
(tRNA-Ser(gct))

SrishMeg2525_84 — tRNA
(tRNA-Ser(cag))

SrishMeg2525_85 — tRNA
(tRNA-Leu(gag))

SrishMeg2525_86 — tRNA
(tRNA-Leu(caa))

SrishMeg2525_87

SrishMeg2525_88

SrishMeg2525_89
(portal protein)

SrishMeg2525_90

SrishMeg2525_91

SrishMeg2525_92
(ThyX-like thymidylate synthase)

SrishMeg2525_93

SrishMeg2525_94
(RNA ligase)

SrishMeg2525_95

SrishMeg2525_96

SrishMeg2525_97

SrishMeg2525_98
(major capsid protein)

SrishMeg2525_99
(Holliday junction resolvase)

SrishMeg2525_100

SrishMeg2525_101
(head fiber protein)

SrishMeg2525_102

SrishMeg2525_103

SrishMeg2525_104

SrishMeg2525_105

SrishMeg2525_106

SrishMeg2525_107

SrishMeg2525_108

SrishMeg2525_109

SrishMeg2525_110

SrishMeg2525_111

SrishMeg2525_112

SrishMeg2525_113

SrishMeg2525_114

SrishMeg2525_115

SrishMeg2525_116

SrishMeg2525_117

SrishMeg2525_118

SrishMeg2525_119

SrishMeg2525_120
(acetyltransferase)

SrishMeg2525_121

SrishMeg2525_122

SrishMeg2525_123

SrishMeg2525_124

SrishMeg2525_125

SrishMeg2525_126
(tail sheath protein)

SrishMeg2525_127

SrishMeg2525_128

SrishMeg2525_129
(tail assembly chaperone)

SrishMeg2525_130
(tail assembly chaperone)

SrishMeg2525_131
(tape measure protein)

SrishMeg2525_132
(minor tail protein)

SrishMeg2525_133

SrishMeg2525_134

SrishMeg2525_135
(minor tail protein)

SrishMeg2525_136

SrishMeg2525_137

SrishMeg2525_138
(baseplate wedge protein)

SrishMeg2525_139
(baseplate wedge protein)

SrishMeg2525_140

SrishMeg2525_141

SrishMeg2525_142

SrishMeg2525_143
(minor tail protein)

SrishMeg2525_144
(minor tail protein)

SrishMeg2525_145

SrishMeg2525_146

SrishMeg2525_147

SrishMeg2525_148

SrishMeg2525_149

SrishMeg2525_150

SrishMeg2525_151

SrishMeg2525_152 — tRNA
(tRNA-Pro(tgg))

SrishMeg2525_153 — tRNA
(tRNA-Trp(cca))

SrishMeg2525_154

SrishMeg2525_155
(peptidyl tRNA hydrolase)

SrishMeg2525_156 — tRNA
(tRNA-Pyl(cta))

SrishMeg2525_157 — tRNA
(tRNA-Met(cat))

SrishMeg2525_158 — tRNA
(tRNA-Cys(gca))

SrishMeg2525_159 — tRNA
(tRNA-Glu(ctc))

SrishMeg2525_160 — tRNA
(tRNA-His(gtg))

SrishMeg2525_161 — tRNA
(tRNA-Ala(tgc))

SrishMeg2525_162 — tRNA
(tRNA-Phe(gaa))

SrishMeg2525_163 — tRNA
(tRNA-Val(cac))

SrishMeg2525_164 — tRNA
(tRNA-Lys(ctt))

SrishMeg2525_165 — tRNA
(tRNA-Glu(ttc))

SrishMeg2525_166 — tRNA
(tRNA-Gly(tcc))

SrishMeg2525_167 — tRNA
(tRNA-Thr(cgt))

SrishMeg2525_168 — tRNA
(tRNA-Thr(tgt))

SrishMeg2525_169 — tRNA
(tRNA-Thr(ggt))

SrishMeg2525_170
(minor tail protein)

SrishMeg2525_171

SrishMeg2525_172 — tRNA
(tRNA-Gly(gcc))

SrishMeg2525_173 — tRNA
(tRNA-Asp(gtc))

SrishMeg2525_174 — tRNA
(tRNA-Met(cat))

SrishMeg2525_175 — tRNA
(tRNA-Ile(gat))

SrishMeg2525_176 — tRNA
(tRNA-Arg(acg))

SrishMeg2525_177 — tRNA
(tRNA-Val(gac))

SrishMeg2525_178 — tRNA
(tRNA-Arg(cct))

SrishMeg2525_179 — tmRNA
(Tag peptide: ATDTDATVTDAEIEAFFAEEAAALV*)

SrishMeg2525_180 — tRNA
(tRNA-Gln(ttg))

SrishMeg2525_181 — tRNA
(tRNA-Arg(tct))

SrishMeg2525_182 — tRNA
(tRNA-Lys(ttt))

SrishMeg2525_183

SrishMeg2525_184

SrishMeg2525_185
(DNA helicase)

SrishMeg2525_186

SrishMeg2525_187

SrishMeg2525_188

SrishMeg2525_189

SrishMeg2525_190

SrishMeg2525_191

SrishMeg2525_192

SrishMeg2525_193

SrishMeg2525_194

SrishMeg2525_195
(thymidylate kinase)

SrishMeg2525_196
(aminotransferase)

SrishMeg2525_197

SrishMeg2525_198

SrishMeg2525_199
(DnaC-like helicase loader)

SrishMeg2525_200
(DnaB-like dsDNA helicase)

SrishMeg2525_201

SrishMeg2525_202
(DNA primase)

SrishMeg2525_203

SrishMeg2525_204
(DnaJ-like chaperonin)

SrishMeg2525_205
(SSB protein)

SrishMeg2525_206
(DnaE-like DNA polymerase III alpha)

SrishMeg2525_207
(RF-1 peptide chain release factor)

SrishMeg2525_208
(RecA-like DNA recombinase)

SrishMeg2525_209
(Holliday junction resolvase)

SrishMeg2525_210

SrishMeg2525_211
(HNH endonuclease)

SrishMeg2525_212
(RusA-like resolvase)

SrishMeg2525_213

SrishMeg2525_214

SrishMeg2525_215

SrishMeg2525_216

SrishMeg2525_217

SrishMeg2525_218

SrishMeg2525_219

SrishMeg2525_220 — tRNA
(tRNA-Gln(ctg))

SrishMeg2525_221 — tRNA
(tRNA-Asn(gtt))

SrishMeg2525_222

SrishMeg2525_223

SrishMeg2525_224

SrishMeg2525_225

SrishMeg2525_226

SrishMeg2525_227

SrishMeg2525_228

SrishMeg2525_229

SrishMeg2525_230
(Ro-like RNA binding protein)

SrishMeg2525_231

SrishMeg2525_232

SrishMeg2525_233

SrishMeg2525_234

SrishMeg2525_235

SrishMeg2525_236

SrishMeg2525_237
(glycosyltransferase)

SrishMeg2525_238

SrishMeg2525_239
(glycosyltransferase)

SrishMeg2525_240
(glycosyltransferase)

SrishMeg2525_241
(PnuC-like nicotinamide riboside transporter)

SrishMeg2525_242
(glycosyltransferase)

SrishMeg2525_243

SrishMeg2525_244

SrishMeg2525_245

SrishMeg2525_246

SrishMeg2525_247
(HNH endonuclease)

SrishMeg2525_248
(lysin A)

SrishMeg2525_249

SrishMeg2525_250
(lysin B)

SrishMeg2525_251
(terminase)

SrishMeg2525_252

SrishMeg2525_253

SrishMeg2525_254

SrishMeg2525_255

SrishMeg2525_256
(polynucleotide kinase)

SrishMeg2525_257

SrishMeg2525_258

SrishMeg2525_259
(serine/threonine kinase)

SrishMeg2525_260

SrishMeg2525_261

SrishMeg2525_262
(PurA-like adenylosuccinate synthetase)

SrishMeg2525_263

SrishMeg2525_264

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR521070
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.18
Date of SEA Lysate Titering Nov 10, 2015
Pitt Freezer Box# 26
Pitt Freezer Box Grid# D8
Available Files
EM PictureDownload
GenBank File for PhameratorDownload