Mycobacterium phage BobaPhett
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Detailed Information for Phage BobaPhett
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAnnette Kang
Year Found2015
Location FoundPhiladelphia, PA United States
Finding InstitutionDrexel University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates39.957689 N, 75.18926 W Map
Discovery NotesDiscovered within the deep moist depths of the Earth next to a bushbed near the Buckley Tennis Courts on the Drexel University Campus, Boba Phett had a rough childhood, having been brought up in a rough neighborhood surrounded by constant foot traffic and uncomfortable amounts of human perspiration and other unmentionable fluids excreted by the human body.
Naming NotesA long time ago, in a galaxy far far away...existed Boba Phett, the ultimate bacteria bounty hunter.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 13, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1397
Genome length (bp)59815
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGAAGGCGC
GC Content61.3%
Sequencing Notesmixed sample, 16% reads Cluster B1
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

BabytheFifth (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

BigPop (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

BoomCrackaPop (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

Buffalo (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clifton (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Estella (F1)

Extra (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Forneus (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GreatPumpkin (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Judylove (F1)

Kalikimaka (F1)

Katya (F1)

Kersh (F1)

KewGel (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

Latretium (F1)

LillyLatzon (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

Mille (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

Noodle (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StacysMom (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TonaldTDrump (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Viva (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotation StatusIn GenBank
Plaque Notes- approx. 3.5-4mm
- clear center with very slight haze surrounding the inner circumference of the phage
- moderately aggressively in terms of growth rate since the size is relatively small in comparison to how much bacterial lawn was cleared during each infection ( a silent but deadly killer)
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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BobaPhett_1
(Terminase, small subunit)

BobaPhett_2
(Terminase, Large Subunit)

BobaPhett_3
(Portal protein)

BobaPhett_4
(Capsid Maturation Protease)

BobaPhett_5
(Scaffolding protein)

BobaPhett_6
(Major capsid protein)

BobaPhett_7

BobaPhett_8

BobaPhett_9

BobaPhett_10

BobaPhett_11
(major tail protein)

BobaPhett_12
(Tail assembly chaperone)

BobaPhett_13
(Tail assembly chaperone)

BobaPhett_14
(Tape measure protein)

BobaPhett_15
(Minor tail protein)

BobaPhett_16
(Minor tail protein)

BobaPhett_17
(Minor tail protein)

BobaPhett_18
(Minor tail protein)

BobaPhett_19
(Minor tail protein, D-ala-D-ala carboxypeptidase)

BobaPhett_20
(Minor tail protein)

BobaPhett_21

BobaPhett_22

BobaPhett_23

BobaPhett_24

BobaPhett_25
(HTH DNA binding protein)

BobaPhett_26

BobaPhett_27

BobaPhett_28

BobaPhett_29

BobaPhett_30
(Lysin A)

BobaPhett_31
(Lysin B)

BobaPhett_32
(Holin)

BobaPhett_33

BobaPhett_34

BobaPhett_35
(DnaQ-like exonuclease)

BobaPhett_36

BobaPhett_37

BobaPhett_38

BobaPhett_39

BobaPhett_40

BobaPhett_41

BobaPhett_42

BobaPhett_43
(Integrase (Y-int))

BobaPhett_44
(toxin in toxin/antitoxin system, RelE-like)

BobaPhett_45
(Antitoxin in toxin/antitoxin system (RelB-like))

BobaPhett_46

BobaPhett_47
(Immunity repressor)

BobaPhett_48
(HTH DNA-binding domain protein)

BobaPhett_49

BobaPhett_50

BobaPhett_51

BobaPhett_52

BobaPhett_53

BobaPhett_54
(helix-turn-helix DNA binding domain)

BobaPhett_55

BobaPhett_56

BobaPhett_57

BobaPhett_58

BobaPhett_59

BobaPhett_60

BobaPhett_61
(WhiB family transcription factor)

BobaPhett_62

BobaPhett_63
(WhiB family transcription factor)

BobaPhett_64
(Helix-Turn-Helix DNA Binding Domain)

BobaPhett_65

BobaPhett_66

BobaPhett_67

BobaPhett_68
(RecA-like DNA recombinase)

BobaPhett_69
(HNH endonuclease)

BobaPhett_70
(DNA helicase)

BobaPhett_71
(DNA methylase)

BobaPhett_72

BobaPhett_73

BobaPhett_74

BobaPhett_75

BobaPhett_76
(GIY-YIG homing endonuclease)

BobaPhett_77
(HTH DNA binding)

BobaPhett_78

BobaPhett_79

BobaPhett_80

BobaPhett_81

BobaPhett_82

BobaPhett_83
(Mycobacteriophage Mobile Element 1 (MPME 1))

BobaPhett_84

BobaPhett_85
(HTH DNA binding domain)

BobaPhett_86

BobaPhett_87

BobaPhett_88

BobaPhett_89

BobaPhett_90

BobaPhett_91

BobaPhett_92

BobaPhett_93
(GIY-YIG endonuclease)

BobaPhett_94
(DNA binding domain)

BobaPhett_95

BobaPhett_96

BobaPhett_97

BobaPhett_98

BobaPhett_99

BobaPhett_100

BobaPhett_101

BobaPhett_102

BobaPhett_103

BobaPhett_104
(Glycosyltransferase)

BobaPhett_105

BobaPhett_106
(serine/threonine kinase)

BobaPhett_107

BobaPhett_108
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH155865
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
GenBank File for PhameratorDownload