Mycobacterium phage Phatniss
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Detailed Information for Phage Phatniss
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTaylor Veralli
Year Found2013
Location FoundBaltimore, MD United States
Finding InstitutionJohns Hopkins University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates39.331389 N, 76.619444 W Map
Discovery NotesMy phage was obtained from very dry soil near an area with a lot of foot traffic.
Naming NotesKatniss Everdeen is a character from the Hunger Games who had to overcome a lot of fictional adversity. I name my phage in her honor. Also my phage is very active (individual plaques were not visible from streaking, and my HTL was drawn from web plate diluted all the way to 10^-5) so the name, phonetically sounding like "Fat"niss, is also applicable because it enjoys its fair share of bacteria.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 21, 2014
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage840
Sanger Finishing Reactions4
Genome length (bp)57293
Character of genome ends3' Sticky Overhang
Overhang Length10 bp
Overhang SequenceCGGAAGGCGC
GC Content61.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

BuzzLyseyear (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LillyLatzon (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nitzel (F1)

Noodle (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phabio (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Polka14 (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

Plaque NotesThe plagues have the appearance of a "bulls eye." The average size is 3 mm in diameter, but this varies greatly depending on the concentration of phage. At lower concentrations the plagues are around 5mm in diameter, and at the web plate concentrations the diameter of each plaque is around 1-1.5 mm.
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes101
Has been Phamerated?Yes
Gene List

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Phatniss_1
(Terminase, small subunit)

Phatniss_2
(Terminase, large subunit)

Phatniss_3
(Portal)

Phatniss_4
(Capsid maturation protease)

Phatniss_5
(Scaffolding)

Phatniss_6
(Major capsid)

Phatniss_7
(Head-to-tail connector)

Phatniss_8
(Head-to-tail connector)

Phatniss_9
(Head-to-tail connector)

Phatniss_10
(Head-to-tail connector)

Phatniss_11
(Major tail subunit)

Phatniss_12
(Tail assembly chaperone)

Phatniss_13
(Tail assembly chaperone)

Phatniss_14
(HNH homing endonuclease)

Phatniss_15
(Tapemeasure)

Phatniss_16
(Minor tail)

Phatniss_17
(Minor tail)

Phatniss_18
(Minor tail)

Phatniss_19

Phatniss_20
(D-ala D-ala carboxypeptidase)

Phatniss_21

Phatniss_22

Phatniss_23

Phatniss_24
(Minor tail)

Phatniss_25

Phatniss_26
(HTH DNA-binding)

Phatniss_27

Phatniss_28

Phatniss_29

Phatniss_30

Phatniss_31
(Lysin A)

Phatniss_32
(Lysin B)

Phatniss_33
(Holin)

Phatniss_34

Phatniss_35

Phatniss_36
(Dna-Q like exonuclease)

Phatniss_37

Phatniss_38

Phatniss_39

Phatniss_40

Phatniss_41
(Integrase, Y)

Phatniss_42

Phatniss_43
(Repressor)

Phatniss_44
(CRO)

Phatniss_45
(AntA antirepressor)

Phatniss_46

Phatniss_47

Phatniss_48

Phatniss_49
(Xis (recombination directionality factor))

Phatniss_50

Phatniss_51

Phatniss_52

Phatniss_53

Phatniss_54

Phatniss_55
(WhiB)

Phatniss_56

Phatniss_57
(WhiB)

Phatniss_58
(HTH DNA binding)

Phatniss_59

Phatniss_60

Phatniss_61

Phatniss_62
(HTH DNA binding)

Phatniss_63
(HNH endonuclease)

Phatniss_64

Phatniss_65

Phatniss_66

Phatniss_67

Phatniss_68
(tRNA methyl transferase)

Phatniss_69
(GIY-YIG homing endonuclease)

Phatniss_70
(DNA binding)

Phatniss_71

Phatniss_72

Phatniss_73

Phatniss_74

Phatniss_75

Phatniss_76

Phatniss_77

Phatniss_78

Phatniss_79

Phatniss_80

Phatniss_81

Phatniss_82

Phatniss_83

Phatniss_84

Phatniss_85

Phatniss_86

Phatniss_87

Phatniss_88

Phatniss_89

Phatniss_90

Phatniss_91

Phatniss_92
(HTH DNA binding)

Phatniss_93

Phatniss_94

Phatniss_95

Phatniss_96

Phatniss_97

Phatniss_98
(glycosyltransferase)

Phatniss_99

Phatniss_100
(glycosyltransferase)

Phatniss_101
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT279576
Refseq NumberNone yet
Available Files
Plaque PictureDownload
EM PictureDownload