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Mycobacterium phage Seagreen
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Detailed Information for Phage Seagreen
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCarlyle Seagreen
Year Found2014
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates29.817338 S, 30.944967 E Map
Discovery NotesSoil sample was taken from a flower bed wherein the soil was relatively dry. The sample was enriched, after which the phages were seen. Taken from the surface of the soil.
Naming NotesThe name was taken from the finders surname. (Seagreen)
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 21, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2910
Genome length (bp)57766
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTAGGCGC
GC Content61.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

Click to View

AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

BuzzLyseyear (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LillyLatzon (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nitzel (F1)

Noodle (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Polka14 (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

Plaque NotesThe plaques have an inner circle that is clear, measuring 2 millimeters. Aftr 36-48 hours the plaques form a clear halo. Including the halo the plaque measures 5mm.
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes105
Has been Phamerated?Yes
Gene List

Click to View

Seagreen_1

Seagreen_2
(Terminase, small subunit)

Seagreen_3
(Terminase, large subunit)

Seagreen_4
(Portal)

Seagreen_5
(Capsid maturation protease)

Seagreen_6
(Scaffolding)

Seagreen_7
(Major Capsid)

Seagreen_8
(Head-to-tail connector)

Seagreen_9
(Head-to-tail connector)

Seagreen_10
(head-to-tail connector)

Seagreen_11
(Head-to-tail connector)

Seagreen_12
(Major Tail)

Seagreen_13
(Tail assembly chaperone)

Seagreen_14
(Tail assambly chaperone)

Seagreen_15
(Tape measure)

Seagreen_16
(minor tail)

Seagreen_17
(minor tail)

Seagreen_18
(minor tail)

Seagreen_19
(minor tail)

Seagreen_20
(minor tail, D-alaD-ala decarboxylase)

Seagreen_21

Seagreen_22

Seagreen_23

Seagreen_24

Seagreen_25

Seagreen_26

Seagreen_27

Seagreen_28

Seagreen_29

Seagreen_30

Seagreen_31
(Lysin A)

Seagreen_32
(Lysin B)

Seagreen_33
(Holin)

Seagreen_34

Seagreen_35

Seagreen_36
(DnaQ-like exonuclease)

Seagreen_37

Seagreen_38

Seagreen_39

Seagreen_40

Seagreen_41
(HTH DNA binding protein)

Seagreen_42

Seagreen_43

Seagreen_44
(Integrase (Y-Int))

Seagreen_45

Seagreen_46
(Immunity repressor)

Seagreen_47
(CRO)

Seagreen_48
(DNA binding protein)

Seagreen_49

Seagreen_50
(NKF)

Seagreen_51
(GPNU1 DBD;, terminase small subunit; DNA binding domain, homodimer, viral assembly, winged helix-turn-helix, viral protein; NMR {Enterobacteria phage lambda})

Seagreen_52
(Geminin; human, DNA replication, cell cycle; 2.00A {Homo sapiens})

Seagreen_53
(NKF)

Seagreen_54
(NKF)

Seagreen_55
(NKF)

Seagreen_56
(NKF)

Seagreen_57
(WhiB family transcription factor)

Seagreen_58
(HTH DNA binding protein)

Seagreen_59
(HTH DNA binding protein)

Seagreen_60
(WhiB family transcription regulator)

Seagreen_61

Seagreen_62

Seagreen_63

Seagreen_64
(HNH endonuclease domain)

Seagreen_65

Seagreen_66

Seagreen_67

Seagreen_68
(DNA methylase)

Seagreen_69
(DNA binding protein)

Seagreen_70

Seagreen_71

Seagreen_72
(DNA methylase)

Seagreen_73
(DNA endonuclease (vsr))

Seagreen_74
(DNA Methylase domain)

Seagreen_75

Seagreen_76

Seagreen_77

Seagreen_78

Seagreen_79

Seagreen_80

Seagreen_81

Seagreen_82

Seagreen_83

Seagreen_84

Seagreen_85

Seagreen_86

Seagreen_87

Seagreen_88

Seagreen_89

Seagreen_90

Seagreen_91

Seagreen_92

Seagreen_93

Seagreen_94

Seagreen_95

Seagreen_96

Seagreen_97

Seagreen_98

Seagreen_99

Seagreen_100

Seagreen_101

Seagreen_102
(glycosyltransferase)

Seagreen_103
(Ser/Thr kinase)

Seagreen_104

Seagreen_105
(glycosyltransferase)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT281793
Refseq NumberNone yet
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload