Mycobacterium phage Squirty
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage Squirty
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByErin Osborne
Year Found2012
Location FoundBelleville, MI United States
Finding InstitutionThe Ohio State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.19 N, 83.5 W Map
Discovery NotesIt is from mulch in a garden in my front years. It was from about an inch underground. The soil was dry. The temperature that day was 84 degrees Fahrenheit and was 90% humidity.
Naming NotesIt was my nickname as a child.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 30, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage430
Sanger Finishing Reactions11
Genome length (bp)60285
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCGACGGCAT
GC Content62.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF3
Cluster Life CycleTemperate
Other Cluster Members

Click to View

AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

BabytheFifth (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

BoomCrackaPop (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clifton (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GreatPumpkin (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Judylove (F1)

Kalikimaka (F1)

Katya (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

Latretium (F1)

LillyLatzon (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MerrillCO18 (F1)

Mille (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Myosotis (F1)

Nemain (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

Noodle (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

Taus (F1)

TDanisky (F1)

TonaldTDrump (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotation StatusIn GenBank
Plaque NotesMy plaque was small and clear. It was always about 1mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Squirty_1

Squirty_2
(terminase small subunit)

Squirty_3
(terminase large subunit)

Squirty_4
(portal protein)

Squirty_5
(capsid maturation protease)

Squirty_6
(scaffolding protein)

Squirty_7
(major capsid protein)

Squirty_8
(head-to-tail connector)

Squirty_9
(head-to-tail connector)

Squirty_10
(head-to-tail connector)

Squirty_11
(head-to-tail connector)

Squirty_12
(major tail subunit)

Squirty_13
(tail assembly chaperone)

Squirty_14
(tail assembly chaperone)

Squirty_15
(tapemeasure)

Squirty_16
(minor tail protein)

Squirty_17
(minor tail protein)

Squirty_18
(minor tail protein)

Squirty_19
(minor tail protein)

Squirty_20
(minor tail protein)

Squirty_21

Squirty_22

Squirty_23

Squirty_24
(antitoxin of toxin-antitoxin stability system)

Squirty_25
(cytotoxic translational repressor of toxin-antitoxin stability system)

Squirty_26
(lysin A)

Squirty_27
(holin)

Squirty_28

Squirty_29

Squirty_30

Squirty_31
(RecG-like DNA helicase)

Squirty_32

Squirty_33

Squirty_34
(non-functional integrase)

Squirty_35
(integrase)

Squirty_36

Squirty_37

Squirty_38
(immunity repressor)

Squirty_39
(CRO protein)

Squirty_40
(putative antirepressor)

Squirty_41

Squirty_42

Squirty_43

Squirty_44

Squirty_45

Squirty_46

Squirty_47

Squirty_48

Squirty_49

Squirty_50

Squirty_51

Squirty_52

Squirty_53

Squirty_54

Squirty_55

Squirty_56

Squirty_57

Squirty_58

Squirty_59

Squirty_60
(WhiB)

Squirty_61

Squirty_62

Squirty_63

Squirty_64

Squirty_65

Squirty_66
(AAA ATPase)

Squirty_67
(HNH endonuclease)

Squirty_68
(DNA methylase)

Squirty_69

Squirty_70

Squirty_71

Squirty_72

Squirty_73

Squirty_74

Squirty_75

Squirty_76

Squirty_77

Squirty_78

Squirty_79

Squirty_80

Squirty_81

Squirty_82

Squirty_83

Squirty_84

Squirty_85

Squirty_86

Squirty_87

Squirty_88

Squirty_89

Squirty_90

Squirty_91

Squirty_92

Squirty_93

Squirty_94

Squirty_95

Squirty_96

Squirty_97

Squirty_98

Squirty_99

Squirty_100
(glycosyltransferase)

Squirty_101

Squirty_102
(glycosyltransferase)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKM101124
Refseq NumberNC_026588
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 38
Pitt Freezer Box Grid# 14
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload