Mycobacterium phage Brocalys
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Detailed Information for Phage Brocalys
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJohn Starner and Justin Yen
Year Found2013
Location FoundMontclair, NJ United States of America
Finding InstitutionMontclair State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.865264 N, 74.195513 W Map
Discovery NotesIn front of Richardson Hall
Naming NotesBros + Local, add a -ys ending = brocalys
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 28, 2014
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage108
Sanger Finishing Reactions2
Genome length (bp)54751
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

BuzzLyseyear (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LillyLatzon (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nitzel (F1)

Noodle (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Polka14 (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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Brocalys_1
(terminase, small subunit)

Brocalys_2
(terminase, large subunit)

Brocalys_3
(Portal)

Brocalys_4
(capsid maturation protease)

Brocalys_5
(scaffolding protein)

Brocalys_6
(major capsid protein)

Brocalys_7
(head-to-tail connector)

Brocalys_8
(head-to-tail connector)

Brocalys_9
(head-to-tail connector)

Brocalys_10
(head-to-tail connector)

Brocalys_11
(major tail subunit)

Brocalys_12
(tail assembly chaperone)

Brocalys_13
(tail assembly chaperone)

Brocalys_14
(tape measure protein)

Brocalys_15
(minor tail protein)

Brocalys_16
(minor tail protein)

Brocalys_17
(minor tail protein)

Brocalys_18
(minor tail protein)

Brocalys_19
(minor tail protein)

Brocalys_20
(minor tail protein)

Brocalys_21

Brocalys_22

Brocalys_23

Brocalys_24

Brocalys_25
(Lysin A)

Brocalys_26
(Lysin B)

Brocalys_27
(holin)

Brocalys_28

Brocalys_29

Brocalys_30
(DnaQ-like exonuclease)

Brocalys_31

Brocalys_32

Brocalys_33

Brocalys_34

Brocalys_35

Brocalys_36
(integrase (Y-int))

Brocalys_37
(antitoxin, in toxin-antitoxin system)

Brocalys_38
(toxin, in toxin-antitoxin system)

Brocalys_39

Brocalys_40
(peptidase)

Brocalys_41
(immunity repressor)

Brocalys_42

Brocalys_43
(phage antirepressor protein)

Brocalys_44

Brocalys_45

Brocalys_46

Brocalys_47

Brocalys_48

Brocalys_49

Brocalys_50

Brocalys_51

Brocalys_52
(Transcription factor WhiB)

Brocalys_53

Brocalys_54
(Transcription factor WhiB)

Brocalys_55
(HTH DNA binding protein)

Brocalys_56

Brocalys_57

Brocalys_58

Brocalys_59
(AAA ATPase)

Brocalys_60

Brocalys_61
(DNA Methylase)

Brocalys_62

Brocalys_63

Brocalys_64

Brocalys_65
(GIY-YIG homing endonuclease)

Brocalys_66

Brocalys_67

Brocalys_68

Brocalys_69

Brocalys_70

Brocalys_71

Brocalys_72

Brocalys_73

Brocalys_74

Brocalys_75

Brocalys_76

Brocalys_77

Brocalys_78

Brocalys_79

Brocalys_80

Brocalys_81

Brocalys_82

Brocalys_83
(GIY-YIG homing endonuclease)

Brocalys_84

Brocalys_85

Brocalys_86

Brocalys_87

Brocalys_88

Brocalys_89

Brocalys_90

Brocalys_91

Brocalys_92

Brocalys_93
(glycosyltransferase)

Brocalys_94
(serine/threonine kinase)

Brocalys_95
(Glycosyltransferase)

Brocalys_96
(HNH homing endonuclease)

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKU865302
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Restriction Digest PictureDownload
EM PictureDownload