Mycobacterium phage Melissauren88
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Detailed Information for Phage Melissauren88
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesMelissaLauren
Found ByMelissa and Lauren
Year Found2015
Location FoundNorthampton, MA U.S.A.
Finding InstitutionSmith College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS CoordinatesUnavailable
Discovery NotesWe dug into the soil on the bank of the boathouse docks on Paradise Pond (at about a meter away from the water) and collected at about 2 centimeters deep. We filled the conical tube to about halfway. The soil was darkly colored, very moist, and the water content was high, with an almost muddy texture, but still coarse and clumped, to the soil. The temperature of the air at the time was approximately 20 degrees Celsius. There was very little foot traffic at the docks, because at the time, Paradise Pond was mostly drained, and the docks were not yet being used for much. However, the pond and where we took our soil samples is at the bottom of a short hill where there is a road for cars to travel at the top. The soil was growing thick but dispersed grass about half a meter up of from where we took the sample, and a meter below it, there were little organisms (about 5 mm long) swimming around in the very shallow (about 3 cm deep) water.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 4, 2016
Sequencing FacilitySmith College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage552
Genome length (bp)54356
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

BuzzLyseyear (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LillyLatzon (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nitzel (F1)

Noodle (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Polka14 (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

Plaque NotesPatches of clear plaques were yielded We were able to individually select plaques without too much difficulty
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

Click to View

Melissauren88_1

Melissauren88_2

Melissauren88_3

Melissauren88_4

Melissauren88_5

Melissauren88_6

Melissauren88_7

Melissauren88_8

Melissauren88_9

Melissauren88_10

Melissauren88_11

Melissauren88_12

Melissauren88_13

Melissauren88_14

Melissauren88_15

Melissauren88_16

Melissauren88_17

Melissauren88_18

Melissauren88_19

Melissauren88_20

Melissauren88_21

Melissauren88_22

Melissauren88_23

Melissauren88_24

Melissauren88_25

Melissauren88_26

Melissauren88_27

Melissauren88_28

Melissauren88_29

Melissauren88_30

Melissauren88_31

Melissauren88_32

Melissauren88_33

Melissauren88_34

Melissauren88_35

Melissauren88_36

Melissauren88_37

Melissauren88_38

Melissauren88_39

Melissauren88_40

Melissauren88_41

Melissauren88_42

Melissauren88_43

Melissauren88_44

Melissauren88_45

Melissauren88_46

Melissauren88_47

Melissauren88_48

Melissauren88_49

Melissauren88_50

Melissauren88_51

Melissauren88_52

Melissauren88_53

Melissauren88_54

Melissauren88_55

Melissauren88_56

Melissauren88_57

Melissauren88_58

Melissauren88_59

Melissauren88_60

Melissauren88_61

Melissauren88_62

Melissauren88_63

Melissauren88_64

Melissauren88_65

Melissauren88_66

Melissauren88_67

Melissauren88_68

Melissauren88_69

Melissauren88_70

Melissauren88_71

Melissauren88_72

Melissauren88_73

Melissauren88_74

Melissauren88_75

Melissauren88_76

Melissauren88_77

Melissauren88_78

Melissauren88_79

Melissauren88_80

Melissauren88_81

Melissauren88_82

Melissauren88_83

Melissauren88_84

Melissauren88_85

Melissauren88_86

Melissauren88_87

Melissauren88_88

Melissauren88_89

Melissauren88_90

Melissauren88_91

Melissauren88_92

Melissauren88_93

Melissauren88_94

Melissauren88_95

Melissauren88_96

Melissauren88_97

Melissauren88_98

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet