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Mycobacterium phage Emma
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Detailed Information for Phage Emma
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeams Foster the Phages and 4 Man Wolf Plaque
Year Found2015
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature34°C
GPS Coordinates34.06 N, 118.45 W Map
Discovery NotesSoil sample was collected by Daniela Bradvica from base of a mint plant in the backyard of a residence near UCLA from a depth of 1cm. Phage was isolated,characterized, and annotated by Team Foster the Phage and Team Four Man Wolf Plaque.

Foster the Phage: Alison Abele, Cynthia Araradian, Julia Lee, Brian Rodriguez
Four Man Wolf Plaque: Daniela Bradvica, Wyatt Nelson, Samantha Santopoalo, Lauren Estiandan
TA: Emma Goodwin
Instructor: Jordan Moberg Parker
Naming NotesNamed after the Teaching Assistant for this section.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 11, 2015
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage5610
Genome length (bp)56418
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGAAGGCGC
GC Content61.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

Click to View

AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

BuzzLyseyear (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LillyLatzon (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nitzel (F1)

Noodle (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Polka14 (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

Plaque NotesTwo morphologies:
-Clear plaques, 3 mm diameter
-Bull’s eye plaques, clear center, surrounding turbid region, clear ring on outside, 3 mm
diameter
MorphotypeSiphoviridae
Final annotation complete?No
Number of Genes108
Has been Phamerated?Yes
Gene List

Click to View

Emma_1
(terminase small subunit)

Emma_2
(terminase large subunit)

Emma_3
(portal protein)

Emma_4
(capsid maturation protease)

Emma_5
(scaffolding protein)

Emma_6
(major capsid protein)

Emma_7
(head-to-tail connector protein)

Emma_8
(head-to-tail connector protein)

Emma_9
(head-to-tail connector protein)

Emma_10
(head-to-tail connector protein)

Emma_11
(major tail protein)

Emma_12
(tail assembly chaperone)

Emma_13
(tail assembly chaperone)

Emma_14
(tape measure protein)

Emma_15
(minor tail protein)

Emma_16
(minor tail protein)

Emma_17
(minor tail protein)

Emma_18
(minor tail protein)

Emma_19
(minor tail protein)

Emma_20
(minor tail protein)

Emma_21
(minor tail protein)

Emma_22
(minor tail protein)

Emma_23
(minor tail protein)

Emma_24
(minor tail protein)

Emma_25
(helix-turn-helix DNA binding domain protein)

Emma_26

Emma_27

Emma_28

Emma_29

Emma_30

Emma_31

Emma_32
(lysin A)

Emma_33
(lysin B)

Emma_34
(holin)

Emma_35
(putative membrane protein)

Emma_36

Emma_37
(DNA polymerase III subunit)

Emma_38

Emma_39

Emma_40
(putative membrane protein)

Emma_41
(immunity repressor)

Emma_42

Emma_43

Emma_44
(integrase)

Emma_45

Emma_46
(immunity repressor)

Emma_47
(CRO protein)

Emma_48

Emma_49

Emma_50

Emma_51

Emma_52

Emma_53

Emma_54

Emma_55

Emma_56

Emma_57

Emma_58

Emma_59
(WhiB family transcription factor)

Emma_60

Emma_61
(helix-turn-helix DNA binding domain protein)

Emma_62

Emma_63

Emma_64

Emma_65

Emma_66
(AAA ATPase)

Emma_67
(HNH endonuclease)

Emma_68
(DNA helicase)

Emma_69
(DNA methylase)

Emma_70

Emma_71

Emma_72

Emma_73

Emma_74

Emma_75

Emma_76
(HTH DNA binding protein)

Emma_77

Emma_78

Emma_79

Emma_80

Emma_81

Emma_82

Emma_83

Emma_84

Emma_85

Emma_86

Emma_87
(MPME protein)

Emma_88

Emma_89

Emma_90

Emma_91

Emma_92

Emma_93

Emma_94

Emma_95

Emma_96

Emma_97

Emma_98

Emma_99

Emma_100

Emma_101

Emma_102

Emma_103

Emma_104

Emma_105

Emma_106
(glycosyltransferase)

Emma_107

Emma_108
(glycosyltransferase)

Emma_109
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF668270
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload