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BayC (BG) Nesbitt (BL) Azathoth (AN) Copper (AN) Sibs6 (A1)
Mycobacterium phage Estave1
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Detailed Information for Phage Estave1
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByPaige Estave
Year Found2012
Location FoundKenner, LA United States
Finding InstitutionXavier University of Louisiana
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates29.999444 N, 90.233889 W Map
Discovery NotesThe soil sample was collected at 2400 Kentucky Ave. Kenner, LA 70062. The sample specifically came from a garden containing a plant for egg plants. The soil in the garden was composed of Miracle Gro for flowers and vegetables. There was barely any decaying matter in the area. The soil itself contained very little moisture and was light brown.
Naming NotesThe name of the phage is the founder's last name plus a number associated with its discovery (being the first one found).
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 4, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage340
Sanger Finishing Reactions10
Genome length (bp)60727
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGATGGCGC
GC Content61.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

Click to View

AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

BuzzLyseyear (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LillyLatzon (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nitzel (F1)

Noodle (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Polka14 (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

Plaque NotesThe plaques of Estave1 are small (~0.5mm-1.25 mm), completely clear and circular. There are two distinguishable sizes: small and even smaller.
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes112
Has been Phamerated?Yes
Gene List

Click to View

Estave1_1
(terminase small subunit)

Estave1_2
(Terminase large subunit)

Estave1_3
(Portal)

Estave1_4
(Capsid maturation protein)

Estave1_5
(Scaffolding)

Estave1_6
(major capsid protein)

Estave1_7
(head-to-tail connector protein)

Estave1_8
(head-to-tail connector protein)

Estave1_9
(head-to-tail connector protein)

Estave1_10
(head-to-tail connector protein)

Estave1_11
(Major tail subunit)

Estave1_12
(Tail assembly chaperone)

Estave1_13
(Tail assembly chaperone)

Estave1_14
(Tapemeasure)

Estave1_15
(Minor tail protein)

Estave1_16
(Minor tail protein)

Estave1_17
(Minor tail protein)

Estave1_18
(Minor tail protein)

Estave1_19
(D-ala-D-ala carboxypeptidase)

Estave1_20
(Minor tail protein)

Estave1_21

Estave1_22

Estave1_23
(DNA binding protein)

Estave1_24

Estave1_25

Estave1_26

Estave1_27

Estave1_28
(LysA)

Estave1_29
(LysB)

Estave1_30
(Holin)

Estave1_31

Estave1_32

Estave1_33
(DnaQ-like exonuclease)

Estave1_34

Estave1_35

Estave1_36

Estave1_37

Estave1_38

Estave1_39

Estave1_40

Estave1_41

Estave1_42

Estave1_43
(Integrase Y-int)

Estave1_44

Estave1_45

Estave1_46
(Immunity repressor)

Estave1_47
(HTH DNA binding domain (Cro))

Estave1_48

Estave1_49

Estave1_50

Estave1_51

Estave1_52

Estave1_53

Estave1_54

Estave1_55

Estave1_56

Estave1_57

Estave1_58

Estave1_59

Estave1_60

Estave1_61

Estave1_62
(WhiB)

Estave1_63
(HTH DNA binding protein)

Estave1_64

Estave1_65

Estave1_66

Estave1_67
(AAA ATPase)

Estave1_68

Estave1_69

Estave1_70
(HNH endonuclease)

Estave1_71
(Helicase)

Estave1_72
(DNA methylase)

Estave1_73

Estave1_74

Estave1_75
(DNA methyltransferase)

Estave1_76

Estave1_77

Estave1_78

Estave1_79

Estave1_80

Estave1_81

Estave1_82

Estave1_83

Estave1_84

Estave1_85

Estave1_86

Estave1_87

Estave1_88

Estave1_89

Estave1_90

Estave1_91

Estave1_92

Estave1_93

Estave1_94

Estave1_95

Estave1_96

Estave1_97

Estave1_98

Estave1_99

Estave1_100

Estave1_101

Estave1_102

Estave1_103

Estave1_104

Estave1_105

Estave1_106

Estave1_107

Estave1_108

Estave1_109
(Glycosyltransferase)

Estave1_110

Estave1_111
(Glycosyltransferase)

Estave1_112
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKM279937
Refseq NumberNC_026585
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE