Mycobacterium phage Velveteen
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Detailed Information for Phage Velveteen
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Duplicates of this PhageGoldstein
Found ByJonathan Lapin
Year Found2012
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.441111 N, 79.959722 W Map
Discovery NotesSoil was collected at 9:30 PM, on September 5th, 2012. The outside temperature was 72 degrees Fahrenheit, with humidity. Soil was taken from around a four inch depth. Soil was compact with a limited amount of moisture. Collected from an urban environment - prone to foot traffic and other forms of pollution.
Naming NotesConsidering that "Lapin" in French means rabbit, and that the children's book, "The Velveteen Rabbit," is one of the greatest children's stories, I decided to name this phage, Mycobacteriophage Velveteen.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 9, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage400
Sanger Finishing Reactions4
Genome length (bp)54314
Character of genome ends3' Sticky Overhang
Overhang Length10 bp
Overhang SequenceCCGATGGCAT
GC Content61.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

BuzzLyseyear (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LillyLatzon (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nitzel (F1)

Noodle (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phabio (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Polka14 (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

Plaque NotesPlaques are roughly 1.5 mm in diameter and exhibited a cloudy, turbid characteristic.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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Velveteen_1
(HNH endonuclease)

Velveteen_2
(small subunit of terminase)

Velveteen_3
(Large subunit of Terminase)

Velveteen_4
(Portal)

Velveteen_5
(Capsid maturation protease)

Velveteen_6
(Scaffolding)

Velveteen_7
(Major capsid)

Velveteen_8

Velveteen_9

Velveteen_10

Velveteen_11

Velveteen_12
(Major tail subunit)

Velveteen_13
(Tail assembly chaperone)

Velveteen_14
(Tail assembly chaperone)

Velveteen_15
(Tapemeasure)

Velveteen_16
(Minor tail subunit)

Velveteen_17
(Minor tail protein)

Velveteen_18
(Minor tail subunit)

Velveteen_19
(Minor tail protein)

Velveteen_20

Velveteen_21

Velveteen_22

Velveteen_23

Velveteen_24

Velveteen_25

Velveteen_26
(LysA)

Velveteen_27
(LysB)

Velveteen_28
(holin)

Velveteen_29

Velveteen_30
(DnaQ-like Exonuclease)

Velveteen_31

Velveteen_32

Velveteen_33

Velveteen_34

Velveteen_35

Velveteen_36

Velveteen_37
(Integrase Y-int)

Velveteen_38

Velveteen_39
(Immunity Repressor)

Velveteen_40
(CRO)

Velveteen_41
(DNA damage inducible protein D-like)

Velveteen_42

Velveteen_43

Velveteen_44

Velveteen_45

Velveteen_46

Velveteen_47

Velveteen_48

Velveteen_49

Velveteen_50

Velveteen_51

Velveteen_52
(WhiB)

Velveteen_53
(HTH DNA binding)

Velveteen_54
(HTH DNA binding)

Velveteen_55
(WhiB)

Velveteen_56

Velveteen_57

Velveteen_58

Velveteen_59

Velveteen_60
(RecA)

Velveteen_61
(HNH endonuclease)

Velveteen_62
(DNA helicase/methylase)

Velveteen_63

Velveteen_64

Velveteen_65

Velveteen_66

Velveteen_67

Velveteen_68

Velveteen_69

Velveteen_70

Velveteen_71

Velveteen_72

Velveteen_73

Velveteen_74

Velveteen_75

Velveteen_76

Velveteen_77

Velveteen_78

Velveteen_79

Velveteen_80

Velveteen_81

Velveteen_82

Velveteen_83

Velveteen_84

Velveteen_85

Velveteen_86

Velveteen_87

Velveteen_88

Velveteen_89
(GIY-YIG Endonuclease)

Velveteen_90

Velveteen_91

Velveteen_92

Velveteen_93

Velveteen_94

Velveteen_95

Velveteen_96

Velveteen_97

Velveteen_98
(Polypeptide N-acetylgalactosaminyltransferase)

Velveteen_99

Velveteen_100
(glycosyltransferase)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF017004
Refseq NumberNC_022060
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload