Mycobacterium phage Kersh
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Detailed Information for Phage Kersh
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJoseph Chang
Year Found2014
Location FoundLa Jolla, CA United States
Finding InstitutionUniversity of California, San Diego
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates32.876853 N, 117.239147 W Map
Discovery NotesFrom compost heap of decomposing eucalyptus leaves. Humid weather, compost heap damp. Collected from underneath top layer of soil.
Naming NotesNamed after Clayton Kershaw, Los Angeles Dodgers pitcher.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 12, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1113
Genome length (bp)60190
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTAGGCGC
GC Content61.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

BuzzLyseyear (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LillyLatzon (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nitzel (F1)

Noodle (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Polka14 (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

Plaque NotesAfter initial enrichment, the plaque was about 3-4.5 mm in diameter with a turbid outer ring. During early stages of purification however, the phage was only 1-2 mm in diameter with no signs of lysogeny and was extremely clear. Plaque morphology returned to early stages of enrichment during lysates and harvesting.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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Kersh_1
(terminase, small subunit)

Kersh_2
(terminase, large subunit)

Kersh_3
(portal protein)

Kersh_4
(capsid maturation protease)

Kersh_5
(scaffolding protein)

Kersh_6
(major capsid protein)

Kersh_7
(head-to-tail connector protein)

Kersh_8
(head-to-tail connector protein)

Kersh_9
(head-to-tail connector protein)

Kersh_10
(head-to-tail connector protein)

Kersh_11
(major tail subunit)

Kersh_12
(tail assembly chaperone)

Kersh_13
(tail assembly chaperone)

Kersh_14
(tape measure protein)

Kersh_15
(minor tail protein)

Kersh_16
(minor tail protein)

Kersh_17
(minor tail protein)

Kersh_18
(minor tail protein)

Kersh_19
(minor tail protein)

Kersh_20
(minor tail protein, D-ala-D-ala carboxypeptidase)

Kersh_21
(minor tail protein)

Kersh_22

Kersh_23
(HTH DNA binding domain protein)

Kersh_24

Kersh_25

Kersh_26

Kersh_27
(lipase)

Kersh_28

Kersh_29
(lysin A)

Kersh_30
(lysin B)

Kersh_31
(holin)

Kersh_32

Kersh_33

Kersh_34
(DnaQ-like exonuclease)

Kersh_35

Kersh_36

Kersh_37

Kersh_38

Kersh_39

Kersh_40
(HTH DNA binding domain protein)

Kersh_41

Kersh_42

Kersh_43
(integrase, (Y-int))

Kersh_44

Kersh_45

Kersh_46
(immunity repressor)

Kersh_47

Kersh_48
(antirepressor)

Kersh_49

Kersh_50

Kersh_51

Kersh_52

Kersh_53

Kersh_54

Kersh_55

Kersh_56

Kersh_57

Kersh_58

Kersh_59
(WhiB family transcription factor)

Kersh_60

Kersh_61
(WhiB family transcription factor)

Kersh_62
(HTH DNA binding domain protein)

Kersh_63

Kersh_64

Kersh_65

Kersh_66
(RecA DNA Recombinase)

Kersh_67
(HNH endonuclease)

Kersh_68
(DNA methylase)

Kersh_69

Kersh_70

Kersh_71

Kersh_72

Kersh_73

Kersh_74

Kersh_75

Kersh_76

Kersh_77

Kersh_78

Kersh_79

Kersh_80

Kersh_81

Kersh_82

Kersh_83

Kersh_84

Kersh_85

Kersh_86

Kersh_87

Kersh_88

Kersh_89

Kersh_90

Kersh_91

Kersh_92

Kersh_93

Kersh_94

Kersh_95

Kersh_96

Kersh_97
(G-I-Y Y-I-G endonuclease)

Kersh_98

Kersh_99

Kersh_100

Kersh_101

Kersh_102

Kersh_103
(glycosyltransferase)

Kersh_104

Kersh_105
(serine/threonine kinase)

Kersh_106
(glycosyltransferase)

Kersh_107
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX610764
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload