Mycobacterium phage NormanBulbieJr
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Detailed Information for Phage NormanBulbieJr
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGillian Black and Vincent Zecchino
Year Found2017
Location FoundPhiladelphia, PA USA
Finding InstitutionUniversity of the Sciences in Philadelphia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates39.0 N, 75.0 W Map
Discovery NotesWe discovered our phage in the soil outside of our university. The phage was dug up into a plastic bag and promptly isolated using direct isolation.
Naming NotesThis name was chosen to honor one of our lab teaching assistants and for our favorite tool in the phage lab, a bulbie.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 8, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1593
Genome length (bp)58102
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

BabytheFifth (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

BoomCrackaPop (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clifton (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GreatPumpkin (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Judylove (F1)

Kalikimaka (F1)

Katya (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

Latretium (F1)

LillyLatzon (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MerrillCO18 (F1)

Mille (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Myosotis (F1)

Nemain (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

Noodle (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

Taus (F1)

TDanisky (F1)

TonaldTDrump (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotation StatusIn GenBank
Plaque NotesThe morphology of our phage is lytic. This means that it makes very clear plaques. Our phage tends to form plaques of multiple sizes.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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NormanBulbieJr_1
(terminase, small subunit)

NormanBulbieJr_2
(terminase, large subunit)

NormanBulbieJr_3
(portal protein)

NormanBulbieJr_4
(capsid maturation protease)

NormanBulbieJr_5
(scaffolding protein)

NormanBulbieJr_6
(major capsid protein)

NormanBulbieJr_7
(head-to-tail connector complex protein)

NormanBulbieJr_8
(head-to-tail connector complex protein)

NormanBulbieJr_9

NormanBulbieJr_10
(head-to-tail connector complex protein)

NormanBulbieJr_11
(major tail protein)

NormanBulbieJr_12
(tail assembly chaperone)

NormanBulbieJr_13
(tail assembly chaperone)

NormanBulbieJr_14
(tape measure protein)

NormanBulbieJr_15
(minor tail protein)

NormanBulbieJr_16
(minor tail protein)

NormanBulbieJr_17
(minor tail protein)

NormanBulbieJr_18
(minor tail protein)

NormanBulbieJr_19
(minor tail protein, D-ala-D-ala carboxypeptidase)

NormanBulbieJr_20
(minor tail protein)

NormanBulbieJr_21
(minor tail protein)

NormanBulbieJr_22
(minor tail protein)

NormanBulbieJr_23
(minor tail protein)

NormanBulbieJr_24
(minor tail protein)

NormanBulbieJr_25

NormanBulbieJr_26

NormanBulbieJr_27

NormanBulbieJr_28

NormanBulbieJr_29

NormanBulbieJr_30
(lysin A)

NormanBulbieJr_31
(lysin B)

NormanBulbieJr_32
(holin)

NormanBulbieJr_33

NormanBulbieJr_34

NormanBulbieJr_35
(DnaQ-like exonuclease (DNA polymerase III subunit))

NormanBulbieJr_36

NormanBulbieJr_37

NormanBulbieJr_38

NormanBulbieJr_39

NormanBulbieJr_40

NormanBulbieJr_41

NormanBulbieJr_42

NormanBulbieJr_43
(transposase)

NormanBulbieJr_44
(integrase)

NormanBulbieJr_45

NormanBulbieJr_46

NormanBulbieJr_47
(immunity repressor)

NormanBulbieJr_48

NormanBulbieJr_49

NormanBulbieJr_50

NormanBulbieJr_51

NormanBulbieJr_52

NormanBulbieJr_53

NormanBulbieJr_54

NormanBulbieJr_55

NormanBulbieJr_56

NormanBulbieJr_57
(WhiB family transcription factor)

NormanBulbieJr_58

NormanBulbieJr_59
(WhiB family transcription factor)

NormanBulbieJr_60
(helix-turn-helix DNA binding domain)

NormanBulbieJr_61

NormanBulbieJr_62

NormanBulbieJr_63

NormanBulbieJr_64
(RecA-like DNA recombinase)

NormanBulbieJr_65
(HNH endonuclease)

NormanBulbieJr_66
(DNA helicase)

NormanBulbieJr_67
(DNA methylase)

NormanBulbieJr_68

NormanBulbieJr_69

NormanBulbieJr_70
(G-I-Y Y-I-G endonuclease)

NormanBulbieJr_71

NormanBulbieJr_72

NormanBulbieJr_73

NormanBulbieJr_74

NormanBulbieJr_75

NormanBulbieJr_76
(mycobacteriophage mobile element 1 (MPME 1))

NormanBulbieJr_77

NormanBulbieJr_78

NormanBulbieJr_79

NormanBulbieJr_80

NormanBulbieJr_81

NormanBulbieJr_82

NormanBulbieJr_83

NormanBulbieJr_84

NormanBulbieJr_85

NormanBulbieJr_86

NormanBulbieJr_87

NormanBulbieJr_88

NormanBulbieJr_89
(G-I-Y Y-I-G endonuclease)

NormanBulbieJr_90

NormanBulbieJr_91

NormanBulbieJr_92

NormanBulbieJr_93

NormanBulbieJr_94

NormanBulbieJr_95

NormanBulbieJr_96

NormanBulbieJr_97

NormanBulbieJr_98

NormanBulbieJr_99
(glycosyltransferase)

NormanBulbieJr_100

NormanBulbieJr_101
(glycosyltransferase)

NormanBulbieJr_102
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH399784
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 47
Pitt Freezer Box Grid# D7
Available Files
GenBank File for PhameratorDownload