Mycobacterium phage Phasih
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Detailed Information for Phage Phasih
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesMufasih
Found ByJames Ramirez
Year Found2015
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.0673 N, 118.4526 W Map
Discovery NotesSample collected by James Ramirez. Depth of sample: 5cm
Sample-site descriptors: Potted Plant in Midvale Plaza, Westwood, soil was moist
Temperature: 70°F

Phage isolated, purified, characterized, and genome annotated by Teams PWA and PITYMI.

Team Phagez wit Attitudez (PWA):
David Chernobylsky
Galen Law
James Ramirez
Julien Trevare

Phage It Til You Make It (PITYMI):
Sean Laiola
Kathy Ordas
Jahzeel Paguntalan
Anna Shvartsur

TA: Fasih Ahsan
Instructor: Giorgia Pirino
Laboratory Support: Krisanavane Reddi
Naming NotesCombination of Mufasah from the Lion King and Fasih, the section Teaching Assistant.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 4, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage354
Genome length (bp)56125
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGAAGGCGC
GC Content61.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

Click to View

AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

BuzzLyseyear (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LillyLatzon (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nitzel (F1)

Noodle (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Polka14 (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

Plaque NotesClear plaques
Average plaque size: 2.74mm
MorphotypeSiphoviridae
Final annotation complete?No
Number of Genes99
Has been Phamerated?Yes
Gene List

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Phasih_1
(terminase small subunit)

Phasih_2
(terminase large subunit)

Phasih_3
(portal protein)

Phasih_4
(capsid maturation protease)

Phasih_5
(scaffolding protein)

Phasih_6
(major capsid protein)

Phasih_7
(head-to-tail connector protein)

Phasih_8
(head-to-tail connector protein)

Phasih_9
(head-to-tail connector protein)

Phasih_10
(head-to-tail connector protein)

Phasih_11
(major tail protein)

Phasih_12
(tail assembly chaperone)

Phasih_13
(tail assembly chaperone)

Phasih_14
(HNH endonuclease)

Phasih_15
(tapemeasure protein)

Phasih_16
(minor tail protein)

Phasih_17
(minor tail protein)

Phasih_18
(minor tail protein)

Phasih_19
(minor tail protein)

Phasih_20
(minor tail protein)

Phasih_21
(minor tail protein)

Phasih_22
(minor tail protein)

Phasih_23
(minor tail protein)

Phasih_24
(minor tail protein)

Phasih_25
(minor tail protein)

Phasih_26
(helix-turn-helix DNA binding domain protein)

Phasih_27

Phasih_28

Phasih_29

Phasih_30

Phasih_31
(lysin A)

Phasih_32
(lysin B)

Phasih_33
(holin)

Phasih_34
(membrane protein)

Phasih_35

Phasih_36
(DNA polymerase III subunit)

Phasih_37

Phasih_38

Phasih_39

Phasih_40

Phasih_41
(integrase)

Phasih_42

Phasih_43
(immunity repressor)

Phasih_44
(CRO protein)

Phasih_45
(antirepressor)

Phasih_46

Phasih_47

Phasih_48

Phasih_49

Phasih_50

Phasih_51

Phasih_52

Phasih_53

Phasih_54

Phasih_55
(WhiB family transcription factor)

Phasih_56

Phasih_57
(WhiB family transcription factor)

Phasih_58
(helix-turn-helix DNA binding domain protein)

Phasih_59

Phasih_60

Phasih_61

Phasih_62
(AAA-ATPase)

Phasih_63

Phasih_64

Phasih_65
(DNA methylase)

Phasih_66

Phasih_67

Phasih_68

Phasih_69

Phasih_70

Phasih_71

Phasih_72

Phasih_73

Phasih_74

Phasih_75

Phasih_76

Phasih_77

Phasih_78

Phasih_79

Phasih_80

Phasih_81

Phasih_82

Phasih_83

Phasih_84

Phasih_85

Phasih_86

Phasih_87

Phasih_88

Phasih_89

Phasih_90

Phasih_91

Phasih_92

Phasih_93

Phasih_94

Phasih_95

Phasih_96

Phasih_97
(glycosyltransferase)

Phasih_98

Phasih_99
(glycosyltransferase)

Phasih_100
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919526
Refseq NumberNone yet
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload